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Dfc 300fx ccd color camera

Manufactured by Leica

The DFC 300FX CCD color camera is a high-performance digital imaging device designed for microscopy applications. It features a CCD (Charge-Coupled Device) sensor that captures color images with high resolution and sensitivity. The camera is capable of producing detailed, accurate, and reliable image data for various scientific and research purposes.

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2 protocols using dfc 300fx ccd color camera

1

Subcellular Localization of PtaHMGB2/3

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For protein subcellular localization, transiently expressing PtaHMGB2/3:YFP and YFP N. benthamiana leaves were sectioned and observed using Zeiss Axiophot epifluorescence microscope. Images were captured with 40× magnification by a Leica DFC 300FX CCD color camera equipped with Leica Application Suite 2.8.1 build 1554 acquisition.
For the quantification of YFP, HMGB2/3:YFP and HMGB6:YFP fluorescence, discs images were captured by a Leica MZ10F fluorescence binocular loupe, using 10× magnification. Images were recorded by a Leica DFC 300FX CCD color camera equipped with Leica Application Suite 2.8.1 build 1554 acquisition. Relative fluorescence (fold-increase) was calculated using Fiji software [77 (link)] as previously described [78 (link)].
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2

Fluorescent Microscopy Protocol

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The slides were examined on a Leica DMR fluorescent microscope equipped with a DFC300 FX CCD color camera using following filter sets: A for DAPI, I3 for Fluorescein-12-dUTP, N2.1 for Cy3, FI/RH for Fluorescein-12-dUTP/Cy3, and B/G/R for blue/green/red fluorescence.
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