For the quantification of YFP, HMGB2/3:YFP and HMGB6:YFP fluorescence, discs images were captured by a Leica MZ10F fluorescence binocular loupe, using 10× magnification. Images were recorded by a Leica DFC 300FX CCD color camera equipped with Leica Application Suite 2.8.1 build 1554 acquisition. Relative fluorescence (fold-increase) was calculated using Fiji software [77 (link)] as previously described [78 (link)].
Dfc 300fx ccd color camera
The DFC 300FX CCD color camera is a high-performance digital imaging device designed for microscopy applications. It features a CCD (Charge-Coupled Device) sensor that captures color images with high resolution and sensitivity. The camera is capable of producing detailed, accurate, and reliable image data for various scientific and research purposes.
2 protocols using dfc 300fx ccd color camera
Subcellular Localization of PtaHMGB2/3
For the quantification of YFP, HMGB2/3:YFP and HMGB6:YFP fluorescence, discs images were captured by a Leica MZ10F fluorescence binocular loupe, using 10× magnification. Images were recorded by a Leica DFC 300FX CCD color camera equipped with Leica Application Suite 2.8.1 build 1554 acquisition. Relative fluorescence (fold-increase) was calculated using Fiji software [77 (link)] as previously described [78 (link)].
Fluorescent Microscopy Protocol
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!