Total cholesterol (T-CHO), triacylglycerol (TG), free fatty acids (FFA), fasting blood glucose (FBG), fasting serum insulin (FINS), acid phosphatase (ACP), alkaline phosphatase (AKP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), catalase (CAT), malondialdehyde (MDA), lipopolysaccharides (LPS) were determined by biochemical kits purchased from Jiancheng Bioengineering Institute (Nanjing, China). The bile salt hydrolase (BSH) content was determined by a biochemical kit purchased from Jianglia Bioengineering Institute (Shanghai, China). The ratio of reduced glutathione (GSH) and oxidized glutathione (GSSG) was determined by a GSH:GSSG kit (Jiancheng Bioengineering Institute, Nanjing, China). Homeostasis model assessment-estimated insulin resistance (HOMA-IR) was calculated using the following formula:
Gsh gssg kit
Manufactured by Nanjing Jiancheng
Sourced in China
The GSH:GSSG kit is a laboratory product used to measure the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) in biological samples. It provides a quantitative assessment of the oxidative status of the sample.
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Lab products found in correlation
2 protocols using gsh gssg kit
1
Metabolic Biomarkers and Insulin Resistance
2
Glutathione Redox Status in Substantia Nigra
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The SN tissue blocks were homogenized in a solution provided by the GSH/GSSG kit (Code No. A061-1, Nanjing Jiancheng Bioengineering Institute, China) and then centrifuged at 14,000 × g for 15 min at 4°C. The supernatants from centrifuged homogenates were used to assess the GSH/GSSG ratio following the instructions of the GSH/GSSG kit. GSH and GSSG in the samples were first made to react with 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) to produce a colored reagent. Then, the 2-vinylpyridine reagent was added to the sample supernatant to ensure that GSSG is the only form of glutathione that reacts with the DTNB reagent. Lastly, the GSSG level was subtracted from the total GSH levels to yield the concentration of reduced GSH. GSH and GSSG levels in the SN were assessed at 412 nm by a spectrophotometer.
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