All chemicals and reagents were purchased from Thermo Fisher Scientific (Waltham, MA) unless otherwise noted. The water used was of Milli-Q grade purified by a Millipore (Bedford, MA) Milli-Q UV Purification System. PCR amplification was performed using Q5 high fidelity DNA polymerase (New England Biolabs; Ipswich, MA). Digestions used FastDigest enzymes (Thermo Fisher Scientific; Waltham, MA) and ligations used T4 DNA ligase (Life Tech.; Carlsbad, CA).
Milli q uv purification system
Manufactured by Merck Group
Sourced in United Kingdom, United States
The Milli-Q UV Purification System is a water purification device designed to produce high-purity water for laboratory applications. It utilizes a combination of filtration and ultraviolet (UV) light treatment to remove impurities and microorganisms from the input water source, resulting in water that meets the stringent quality requirements of various laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
T4 dna ligase, by Thermo Fisher Scientific (1 mentions)
Fastdigest enzymes, by Thermo Fisher Scientific (1 mentions)
Q5 high fidelity dna polymerase, by New England Biolabs (1 mentions)
Ammonium acetate, by Avantor (1 mentions)
Trifluoroacetic acid, by Avantor (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Methanol, by Avantor (1 mentions)
Phthalimide, by Merck Group (1 mentions)
Chloroform, by Thermo Fisher Scientific (1 mentions)
Gc grade hexane, by Thermo Fisher Scientific (1 mentions)
0.1 mm zirconium silica beads, by Biospec (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
3 protocols using milli q uv purification system
1
Molecular Biology Techniques Utilized
2
Analytical Testing and Internal Standard Protocol
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TAL for analytical testing (purity ≥ 99%) and phthalimide (purity ≥ 99%), used as internal standard (IS), were provided by Sigma-Aldrich (USA). Ammonium acetate, methanol (CH3OH), acetonitrile (CH3CN) and trifluoroacetic acid (98%) were purchased from VWR International (USA). Acetic acid 99–100% (CH3COOH) was obtained from J.T. Baker (USA). The water used was ultrapure grade, purified using a Milli- Q UV Purification System (Millipore Corporation, USA).
3
Extraction and Analysis of Metabolites
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All chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted. HPLC-grade methanol, chloroform and GC-grade hexane were purchased from Fisher Scientific (Fair Lawn, NJ). Zirconium/silica beads (0.1 mm) were purchased from Biospec Products (Bartlesville, OK). The water used was of Milli-Q grade purified by a Millipore (Bedford, MA) Milli-Q UV Purification System.
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