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Anti total β catenin and anti parp antibodies

Manufactured by Merck Group

Anti-total β-catenin and anti-PARP antibodies are laboratory reagents used for protein detection and analysis. The anti-total β-catenin antibody is used to detect the β-catenin protein, which plays a role in cellular signaling and gene expression. The anti-PARP antibody is used to detect the PARP protein, which is involved in cellular processes such as DNA repair. These antibodies can be used in various applications, including Western blotting, immunohistochemistry, and immunoprecipitation.

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2 protocols using anti total β catenin and anti parp antibodies

1

Comprehensive Cell Protein Immunoblotting

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Proteins were isolated from cells using cOmplete Lysis M plus PhosSTOP (Roche, Indianapolis, IN). Immunoblotting was carried out as previously described (Rovedo et al. 2011 (link)) Quantification was performed using FujiFilm Multi Gauge software (FujiFilm, Hanover Park, IL). The following Cell Signaling (Danvers, MA) antibodies were used: anti-α-tubulin (DM1A), anti-β-actin (8H10D10), anti-p38α (L53F8), anti-p38β (C28C2), anti-p38γ, anti-p38δ (10A8), anti-p38 MAPK, anit-rabbit IgG HRP-linked secondary, and anti-mouse IgG HRP-linked secondary. Anti-total β-catenin and anti-PARP antibodies were purchased from EMD Millipore and BD Biosciences respectively.
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2

Comprehensive Cell Protein Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were isolated from cells using cOmplete Lysis M plus PhosSTOP (Roche, Indianapolis, IN). Immunoblotting was carried out as previously described (Rovedo et al. 2011 (link)) Quantification was performed using FujiFilm Multi Gauge software (FujiFilm, Hanover Park, IL). The following Cell Signaling (Danvers, MA) antibodies were used: anti-α-tubulin (DM1A), anti-β-actin (8H10D10), anti-p38α (L53F8), anti-p38β (C28C2), anti-p38γ, anti-p38δ (10A8), anti-p38 MAPK, anit-rabbit IgG HRP-linked secondary, and anti-mouse IgG HRP-linked secondary. Anti-total β-catenin and anti-PARP antibodies were purchased from EMD Millipore and BD Biosciences respectively.
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