Anti h1.0 h5 clone 3h9

Manufactured by Merck Group

The Anti-H1.0/H5 clone 3H9 is a laboratory reagent that can be used to detect and study the H1.0 and H5 histone proteins. It is a monoclonal antibody that specifically binds to these histone variants. This product can be used in various laboratory techniques, such as Western blotting, immunoprecipitation, and immunocytochemistry, to investigate the expression and localization of H1.0 and H5 histones.

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Lab products found in correlation

Ab31972, by Abcam (2 mentions) Ab4086, by Thermo Fisher Scientific (1 mentions) Ab24174, by Abcam (1 mentions) Ab24175, by Abcam (1 mentions) Ab1791, by Abcam (1 mentions) Ab8980, by Abcam (1 mentions) Ab11079, by Abcam (1 mentions) Ab8898, by Abcam (1 mentions) Ab4086, by Abcam (1 mentions)

2 protocols using anti h1.0 h5 clone 3h9

Mapping Histone H1 Variants via ChIP-seq

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Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05–629-I), anti-H1.2 (Abcam, ab4086), anti-H1.4 (Invitrogen, 702876), anti-H1.5 (Invitrogen, 711912) and anti-H1X (Abcam, ab31972). ChIP-seq of H3K9me3 was performed using anti-H3K9me3 (Abcam, ab8898). Other antibodies used were: anti-H1.0 (Abcam, ab11079), anti-H1.3 (Abcam, ab24174), anti-H1.5 (Abcam, ab24175), anti-H3 (Abcam, ab1791) and anti-Lamin A (Abcam, ab8980).

Serna-Pujol N., Salinas-Pena M., Mugianesi F., Le Dily F., Marti-Renom M.A, & Jordan A. (2022). Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion. Nucleic Acids Research, 50(7), 3892-3910.

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Antibody Validation for H1 Variants

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Specific antibodies recognizing human H1 variants used for ChIP/ChIP-Seq and immunoblot were: anti-H1.0/H5 clone 3H9 (Millipore, 05–629-I), anti-H1.2 (abcam, ab4086), anti-H1.3 (abcam, ab203948), anti-H1.4 (Invitrogen, 702876), anti-H1.5 (Invitrogen, 711912) and anti-H1X (abcam, ab31972). The variant specificity of anti-H1.3 has been extensively validated in this study (Supplementary Figure S1). Validation of the other anti-H1 antibodies has been reported elsewhere (31 (link)).

Salinas-Pena M., Serna-Pujol N, & Jordan A. (2024). Genomic profiling of six human somatic histone H1 variants denotes that H1X accumulates at recently incorporated transposable elements. Nucleic Acids Research, 52(4), 1793-1813.

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