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Amaxa apparatus

Manufactured by Lonza
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The Amaxa apparatus is a laboratory instrument designed for the electroporation of cells. Electroporation is a technique used to introduce foreign molecules, such as DNA, RNA, or proteins, into cells by creating temporary pores in the cell membrane. The Amaxa apparatus facilitates this process, allowing researchers to efficiently transfect a variety of cell types.

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Lab products found in correlation

Glomax multi detection system, by Promega (1 mentions) Cell line nucleofector kit 5, by Lonza (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions) Pdsred monomer golgi, by Takara Bio (1 mentions) Scramble sirna, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Amaxa Nucleofector apparatus (17 citations) Amaxa Nucleofactor™ apparatus (4 citations) Amaxa Nucleofector II apparatus (2 citations)

7 protocols using amaxa apparatus

1

Targeting CTNNB in BCR-ABL Mutant CML

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Primary BC-CML cells with BCR-ABLT315I/E255V mutations (No.2, Table 1) were electroporated with 750nM CTNNB ON-TARGET plus siRNA (Dharmacon, Lafayette, CO) using an Amaxa apparatus (Solution V, program K-17) (Lonza, Walkersville, MD) following the manufacturer’s instructions. Twenty-four hours after siRNA transfection, cells (0.5×106/ml) were treated with nilotinib for 48h. Apoptosis and cell counts were determined by flow cytometry as described below.
Zhou H., Mak P.Y., Mu H., Mak D.H., Zeng Z., Cortes J., Liu Q., Andreeff M, & Carter B.Z. (2017). Combined inhibition of β-catenin and Bcr–Abl synergistically targets tyrosine kinase inhibitor-resistant blast crisis chronic myeloid leukemia blasts and progenitors in vitro and in vivo. Leukemia, 31(10), 2065-2074.
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2

TGR5 Overexpression and CREB Activation

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Transfections were performed by electroporation using the Amaxa apparatus (Lonza, Switzerland) and Cell Line Nucleofector Kit V (Lonza). The following constructs were transfected in Jurkat T cells: human TGR5 overexpressing plasmid (8), GFP plasmid (PMAX-GFP, Lonza) and CREB-luciferase reporter plasmid (Stratagene, California). Furthermore, we used human Smartpool VDR silencing RNA and control silencing RNA (Dharmacon, Colorado). Luciferase assays were performed using the Dual Luciferase Reporter Assay System (Promega, Wisconsin) kit in the Glomax Multi Detection System (Promega) apparatus. A plasmid encoding renilla luciferase was cotransfected and used as a control for transfection efficiency.
Pols T.W., Puchner T., Korkmaz H.I., Vos M., Soeters M.R, & de Vries C.J. (2017). Lithocholic acid controls adaptive immune responses by inhibition of Th1 activation through the Vitamin D receptor. PLoS ONE, 12(5), e0176715.
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3

Visualizing GFAP Isoforms in Neural Stem Cells

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Human GFAPα cDNA cloned into pEGFP-C2 (hGFAPα-EGFP-C2) or pEGFP-N3 (hGFAPα-EGFP-N3) and human GFAPε cDNA cloned into pEGFP-N3 (hGFAPε-EGFP-N3) were kindly provided by Dr. D. Pham Dinh (Paris, France). These constructions were transfected in mGb4 cells using an Amaxa apparatus and neural stem cell kit (Lonza). For videomicroscopy, the H2B-mCherry plasmid (Addgene, 20972) was co transfected to visualize the nuclei in the red channel. Transfection with pEGFP-N3 (pCMV-EGFP-N3) plasmid (Clontech) was used as control. For visualization of Golgi apparatus, mGb4 cells were transfected with a pDsRed-Monomer-Golgi (Clontech). After 48h, cells were processed for time-lapse confocal microscopy or fixed with 4% paraformaldehyde 20 min RT, for IF.
Guichet P.O., Guelfi S., Ripoll C., Teigell M., Sabourin J.C., Bauchet L., Rigau V., Rothhut B, & Hugnot J.P. (2016). Asymmetric Distribution of GFAP in Glioma Multipotent Cells. PLoS ONE, 11(3), e0151274.
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4

CTNNB1 Silencing in Molm13 and MV4-11 Cells

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Molm13 and MV4-11 cells were electroporated with 750 nM of 4 individual or SMARTpool ON-TARGET plus CTNNB1 siRNAs of the four (Dharmacon, Lafayette, CO) using an Amaxa apparatus (Solution L, program Q-001) (Lonza, Walkersville, MD) following the manufacturer’s instructions. β-catenin silencing was confirmed by western blot analysis.
Jiang X., Mak P.Y., Mu H., Tao W., Mak D.H., Kornblau S., Zhang Q., Ruvolo P., Burks J.K., Zhang W., McQueen T., Pan R., Zhou H., Konopleva M., Cortes J., Liu Q., Andreeff M, & Carter B.Z. (2018). Disruption of Wnt/β-catenin exerts anti-leukemia activity and synergizes with FLT3 inhibition in FLT3-mutant acute myeloid leukemia. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(10), 2417-2429.
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5

Transfecting OCI-AML3 cells with CTNNB1 siRNA

Cited 1 time
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OCI-AML3 cells were electroporated with a scramble control or the SMARTpool ON-TARGET plus CTNNB1 siRNAs (750 nM) (Cat#L-003482–00-0005, Dharmacon; Lafayette, CO) using an Amaxa apparatus (Solution T, Cat#VCA-1002; program X-001) (Lonza; Walkersville, MD) following the manufacturer’s instructions as previously described (28 (link)).
Carter B.Z., Mak P.Y., Wang X., Tao W., Ruvolo V., Mak D., Mu H., Burks J.K, & Andreeff M. (2019). An ARC-regulated IL1β/Cox-2/PGE2/β-catenin/ARC circuit controls leukemia-microenvironment interactions and confers drug resistance in AML. Cancer research, 79(6), 1165-1177.
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6

Assessing Nilotinib Efficacy in CML

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Primary BC-CML cells with BCR–ABLT315I/E255V mutations (no. 2, Table 1) were electroporated with 750 nM CTNNB ON-TARGET plus short interfering RNA (Dharmacon, Lafayette, CO, USA) using an Amaxa apparatus (Solution V, program K-17; Lonza, Walkersville, MD, USA), following the manufacturer’s instructions. Twenty-four hours after short interfering RNA transfection, cells (0.5 × 106/ml) were treated with nilotinib for 48 h. Apoptosis and cell counts were determined by flow cytometry as described below.
Zhou H., Mak P.Y., Mu H., Mak D.H., Zeng Z., Cortes J., Liu Q., Andreeff M, & Carter B.Z. (2017). Combined inhibition of β-catenin and Bcr–Abl synergistically targets tyrosine kinase inhibitor-resistant blast crisis chronic myeloid leukemia blasts and progenitors in vitro and in vivo. Leukemia, 31(10), 2065-2074.
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7

Knockdown of MAP3K14 in AML Cell Lines

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OCI-AML3 and Molm13 cells were electroporated with 10, 50, or 100 nmol/l MAP3K14 siRNA or scramble siRNA (both from Thermo Fisher Scientific, Pittsburgh, PA, USA) using an Amaxa apparatus (Lonza, Walkersville, MD, USA) following the manufacturer’s instructions. The on-TARGETplus SMART pool MAP3K14 siRNA consists of a mixture of four target sequences: GGAUUGACCUCACCCAGAA, GAACCGGGCACUACAGCAA, GUCCAAAUACAGUCUCUUA, GCCAGUGGAUUAUGAGUAC.
Mak P.Y., Mak D.H., Ruvolo V., Jacamo R., Kornblau S.M., Andreeff M, & Carter B.Z. (2014). Apoptosis suppressor ARC modulates SMAC mimetic-induced cell death through BIRC2/MAP3K14 signalling in acute myeloid leukaemia. British journal of haematology, 167(3), 376-384.
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