Antibodies: PGC-1α (Abcam); phospho-Ser1177 eNOS (Upstate Biotechnology); eNOS (BD Transduction); ERRα (Abcam); β-actin (Sigma). Micro-osmotic pumps were purchased from DURECT corporation (Cupertino, CA). Other chemicals were obtained from Sigma.
Micro osmotic pumps
Manufactured by Durect
Sourced in United States
Micro-osmotic pumps are compact, self-contained drug delivery devices that utilize the principle of osmosis to provide controlled, continuous drug administration. These pumps are designed for small-scale, precise drug delivery applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using micro osmotic pumps
1
Molecular Markers of Endothelial Function
2
Leptin Delivery in Obese Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
WT and ob/ob C57BL/6J mice were purchased from Jackson Laboratories. Microosmotic pumps (model 2002; Durect) were used to dispense either leptin (150 ng/h in PBS) or vehicle (PBS). Pumps were implanted to 10-wk-old mice subcutaneously, and mice were single-housed. Recombinant murine leptin was purchased from Amylin Pharmaceuticals.
3
Intraventricular Delivery of Ephrin-B2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At 24 hours after reperfusion, 10% chloral hydrate (0.35 mL/100 g) was used to anesthetize the rats and micro-osmotic pumps (Durect, Cupertino, CA, USA) were implanted subcutaneously into the rats. The head of the rat was stabilized by a stereotaxic apparatus (Stoelting, Wood Dale, IL, USA), punctured at the lateral ventricle, with the anterior fontanelle as the base point of puncture (Kim et al., 2013), anchored at 2 mm to the left side of the midline, 2.6 mm behind the anterior fontanelle and 2 mm in depth. A needle was left in situ for 5 minutes to observe whether the puncture site was bleeding. Then micro-osmotic pumps were inserted at a 2 mm depth by intraventricular administration. Ten micrograms (concentration 100 µg/mL) of recombinant murine ephrin-B2-Fc chimera (R&D Systems, Minneapolis, MN, USA) was diluted with 0.9% sodium chloride and added to an osmotic pump (Durect), which was then injected into the lateral ventricle at a speed of 1 µL/hour for 3 days.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!