Gad65

PTZ, MMP-9 and myricetin were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). PTZ was dissolved in freshly prepared saline prior to treatment (100 mg/ml). Antibodies against cleaved caspase-3 (cat. no. 9661; 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), BDNF (cat. no. PA5-15198: 1:2,000; Invitrogen; Thermo Fishes Scientific, Inc., Waltham, MA, USA), TrkB (cat. no. 4606; 1:1,000; Cell Signaling Technology, Inc.), GABA_AR (cat. no. PA5-78386; 1:1,000; Invitrogen; Thermo Fisher Scientific, Inc.), MMP-9 (cat. no. 3852; 1:1,000; Cell Signaling Technology, Inc.) and GAD65 (cat. no. PA5-22260; 1:2,000; Invitrogen; Thermo Fisher Scientific, Inc.) were obtained and Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) provided antibodies against apoptosis regulator B-cell lymphoma-2 (Bcl-2; cat. no. sc509; 1:1,000), apoptosis regulator Bcl-2-like protein 4 (Bax; cat. no. sc-7480; 1:1,000), Bcl2-associated agonist of cell death (Bad; cat. no. sc-8004; 1:1,000), anti-apoptotic regulator Bcl extra large (Bcl-xL; cat. no. sc-136132; 1:1,000) and β-actin (cat. no. sc-47778; 1:1,000). All other standard chemicals used in the current study were purchased from Sigma-Aldrich; Merck KGaA.