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Sirna asic3

Manufactured by Merck Group
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SiRNA ASIC3 is a lab equipment product designed for the delivery and study of small interfering RNA (siRNA) targeting the ASIC3 gene. The core function of this product is to facilitate the introduction of siRNA molecules into cells to modulate the expression of the ASIC3 gene, which plays a role in various cellular processes. The SiRNA ASIC3 product supports researchers in their investigations related to the ASIC3 gene and its associated pathways.

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Lab products found in correlation

Sirna asic1, by Merck Group (2 mentions) I fect, by Neuromics (2 mentions)

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SiRNAs

2 protocols using sirna asic3

1

Intrathecal Targeting of ASIC Channels

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In the rodent model of RE, PcTx1, APTEx‐2, or vehicle (saline) (1 nmol per rat) was administered intrathecally 15 min before the ED test.24, 25 In separate studies, 10 ml of a siRNA (ASIC1 or ASIC3) (2 μg)/i‐Fect (Neuromics) mix was injected intrathecally using a Hamilton syringe and a 25‐gauge needle. Animals received one injection per day for 3 days before the ED test. SiRNA ASIC1, SiRNA ASIC3 and the corresponding scramble siRNAs were ordered from Sigma. Intrathecal injections were performed freehand under isoflurane anesthesia (2% isoflurane inhalation) between spinal T3 and T5 vertebrae of rats as previously described.26 RT‐PCR studies showed that intrathecal administration of specific ASIC1 and ASIC3 siRNAs resulted in >70% reduction of ASIC1 and 3 expression in the T3‐T5 thoracic dorsal root ganglia.
Han X., Zhang Y., Lee A., Li Z., Gao J., Wu X., Zhao J., Wang H., Chen D., Zou D, & Owyang C. (2021). Upregulation of acid sensing ion channels is associated with esophageal hypersensitivity in GERD. The FASEB Journal, 36(1), e22083.
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2

Intrathecal Modulation of ASIC Receptors in Rodent RE

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In the rodent model of RE, PcTx1, APTEx-2, or vehicle (saline) (1nmol per rat) was administered intrathecally 15 min before the esophageal distension test.24 ,25 (link) In separate studies, ten microliters of a siRNA (ASIC1 or ASIC3) (2ug)/i-Fect (Neuromics) mix was injected intrathecally using a Hamilton syringe and a 25-gauge needle. Animals received one injection per day for 3 days before the esophageal distension test. SiRNA ASIC1, SiRNA ASIC3 and the corresponding scramble siRNAs were ordered from Sigma. Intrathecal injections were performed freehand under isoflurane anesthesia (2% isoflurane inhalation) between spinal T3 and T5 vertebrae of rats as previously described.26 (link) RT-PCR studies showed that intrathecal administration of specific ASIC1 and ASIC3 siRNAs resulted in >70% reduction of ASIC1 and 3 expression in the T-3-T5 thoracic dorsal root ganglia.
Han X., Zhang Y., Lee A., Li Z., Gao J., Wu X., Zhao J., Wang H., Chen D., Zou D, & Owyang C. (2021). Upregulation of acid sensing ion channels is associated with esophageal hypersensitivity in GERD. The FASEB Journal, 36(1), e22083.
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