Penicillin pen

Manufactured by Lonza

Penicillin (PEN) is a laboratory equipment used for the production and purification of the antibiotic penicillin. It provides a controlled environment for the cultivation of the Penicillium fungus and the extraction of the penicillin compound.

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Close spelling variants of this product

Penicillin (1050 citations) Penicillin–streptomycin (Pen-Strep) (8 citations)

2 protocols using penicillin pen

Culture Conditions for 293T and MDCK Cells

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Cells were grown essentially as described in ref. (32 (link)). 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Lonza, Breda, The Netherlands) supplemented with 10% fetal bovine serum (Greiner), 1% nonessential aas (NEAAs) (Lonza), 1 mM sodium pyruvate (Gibco), 2 mM glutamine (L-glu, Lonza), 100 IU/mL penicillin (PEN) (Lonza), and 100 μg/mL streptomycin (STR) (Lonza). Madin–Darby canine kidney (MDCK) cells were cultured in Eagle’s minimal essential medium (Lonza) supplemented with 10% fetal calf serum, 1% NEAAs, 1.5 mg/mL sodium bicarbonate (Lonza), 10 mM Hepes (Lonza), 2 mM glutamine, 100 IU/mL PEN, and 100 μg/mL STR.

Rosu M.E., Lexmond P., Bestebroer T.M., Hauser B.M., Smith D.J., Herfst S, & Fouchier R.A. (2022). Substitutions near the HA receptor binding site explain the origin and major antigenic change of the B/Victoria and B/Yamagata lineages. Proceedings of the National Academy of Sciences of the United States of America, 119(42), e2211616119.

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Cell Culture Preparation for Research

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Madin-Darby canine kidney (MDCK) cells (ATCC) were cultured in Eagle’s minimal essential medium (EMEM) (Lonza) supplemented with 10% fetal bovine serum (FBS) (Greiner), 100 U/ml penicillin (PEN) (Lonza), 100 U/ml streptomycin (STR) (Lonza), 2 mM l-glutamine (l-Glu) (Lonza), 1.5 mg/ml sodium bicarbonate (NaHCO₃) (Lonza), 10 mM HEPES (Lonza) and 1× nonessential amino acids (NEAA) (Lonza). 293T cells were cultured in Dulbecco modified Eagle’s medium (DMEM) (Lonza) supplemented with 10% FBS, 100 U/ml PEN, 100 U/ml STR, 2 mM l-Glu, 1 mM NaHCO₃, and 1× NEAA. Vero cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM) (Lonza) supplemented with 10% FBS, 100 U/ml PEN, 100 mg/ml STR, and 2 mM l-Glu.

Herfst S., Mok C.K., van den Brand J.M., van der Vliet S., Rosu M.E., Spronken M.I., Yang Z., de Meulder D., Lexmond P., Bestebroer T.M., Peiris J.S., Fouchier R.A, & Richard M. (2018). Human Clade 2.3.4.4 A/H5N6 Influenza Virus Lacks Mammalian Adaptation Markers and Does Not Transmit via the Airborne Route between Ferrets. mSphere, 3(1), e00405-17.

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