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Xrn1 a300 443a

Manufactured by Fortis Life Sciences
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Sourced in Hungary

Xrn1 (A300-443A) is a lab equipment product offered by Fortis Life Sciences. It functions as an exoribonuclease that degrades 5' to 3' single-stranded RNA.

Automatically generated - may contain errors

Lab products found in correlation

Ab50558, by Abcam (2 mentions) Ab12286, by Abcam (2 mentions) Ab5642, by Abcam (2 mentions) Ab14601, by Abcam (2 mentions) Nitrocellulose membrane, by GE Healthcare (2 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (2 mentions) Ab50910, by Abcam (2 mentions) Antibody against zap 16820 1 ap, by Proteintech (2 mentions) Gfp g1546 antibody, by Merck Group (2 mentions) Rnase l, by Abcam (1 mentions) Eif2α, by Cell Signaling Technology (1 mentions) α tubulin, by Merck Group (1 mentions) Phospho t446 pkr, by Abcam (1 mentions) Hsc70, by Cayman Chemical (1 mentions) Phospho eif2α ser51, by Cell Signaling Technology (1 mentions) Phosphonoacetic acid paa, by Merck Group (1 mentions) P erk, by Cell Signaling Technology (1 mentions)

3 protocols using xrn1 a300 443a

1

Western Blot Protocol for Protein Detection

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Cells were counted, normalized for cell number, lysed in SDS sample buffer, separated by electrophoresis on NuPage 4–12% Bis-Tris gels (Novex) and blotted onto nitrocellulose membranes (GE Healthcare). Antibodies for PTBP1 (ab5642), Drosha (ab12286), DICR (ab14601), EXOSC6 (ab50910), EXOSC10 (ab50558), and PARN (ab188333) were obtained from Abcam. Antibodies for Upf1 (A300-036A), METTL3 (A301-567A), EXOSC4 (A303-775A), EXOSC5 (A303-887A), and Xrn1 (A300-443A) were obtained from Bethyl Labs. The antibody against ZAP (16820-1-AP) was obtained from Proteintech. The HIV-1 capsid antibody (183-H12-5C) was obtained from the NIH AIDS reagent repository. The GFP (G1546) antibody was obtained from Sigma. The HIV Env (12-6205-1) antibody was obtained from American Research Products. The HA (HA.11) antibody used in the CLIP assays was obtained from Biolegend.
Takata M.A., Gonçalves-Carneiro D., Zang T., Soll S.J., York A., Blanco-Melo D, & Bieniasz P.D. (2017). CG-dinucleotide suppression enables antiviral defense targeting non-self RNA. Nature, 550(7674), 124-127.
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2

Antibody Sources for Protein Analysis

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Monoclonal E3L antiserum was a kind gift from S. Isaacs (Univ. Pennsylvania, PA). Polyclonal PABP1 antiserum was a kind gift of S. Morley (Univ. Sussex, UK). I3 antiserum was a kind gift of D. Evans (Univ. Alberta, Canada). All other antibodies were purchased commercially, as follows: Xrn1 (A300-443A; Bethyl Labs), α tubulin (6074; Sigma), eIF2α (5324; Cell Signaling), phospho(ser51)-eIF2α (3398; Cell Signaling), PKR (12297; Cell Signaling), phospho(T446)-PKR (32036; Abcam), PERK (5683; Cell Signaling), Hsc70 (10011384; Cayman Chemical); RNase L (13825; Abcam), dsRNA (J2; SciCons, Hungary). Phosphonoacetic acid (PAA) was from Sigma.
Burgess H.M, & Mohr I. (2015). Cellular 5′-3′ mRNA Exonuclease Xrn1 Controls Double-Stranded RNA Accumulation and Anti-Viral Responses. Cell Host & Microbe, 17(3), 332-344.
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3

Western Blot Protocol for Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were counted, normalized for cell number, lysed in SDS sample buffer, separated by electrophoresis on NuPage 4–12% Bis-Tris gels (Novex) and blotted onto nitrocellulose membranes (GE Healthcare). Antibodies for PTBP1 (ab5642), Drosha (ab12286), DICR (ab14601), EXOSC6 (ab50910), EXOSC10 (ab50558), and PARN (ab188333) were obtained from Abcam. Antibodies for Upf1 (A300-036A), METTL3 (A301-567A), EXOSC4 (A303-775A), EXOSC5 (A303-887A), and Xrn1 (A300-443A) were obtained from Bethyl Labs. The antibody against ZAP (16820-1-AP) was obtained from Proteintech. The HIV-1 capsid antibody (183-H12-5C) was obtained from the NIH AIDS reagent repository. The GFP (G1546) antibody was obtained from Sigma. The HIV Env (12-6205-1) antibody was obtained from American Research Products. The HA (HA.11) antibody used in the CLIP assays was obtained from Biolegend.
Takata M.A., Gonçalves-Carneiro D., Zang T., Soll S.J., York A., Blanco-Melo D, & Bieniasz P.D. (2017). CG-dinucleotide suppression enables antiviral defense targeting non-self RNA. Nature, 550(7674), 124-127.
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