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Phos 4ebp1 thr37 46

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Phos-4EBP1 (Thr37/46) is a phospho-specific antibody that recognizes the eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) when phosphorylated at threonine residues 37 and 46. This antibody can be used for the detection and analysis of 4E-BP1 phosphorylation status in various experimental systems.

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Lab products found in correlation

Ab110276, by Abcam (2 mentions) H3k27ac, by Cell Signaling Technology (2 mentions) Gata1, by Abcam (2 mentions) Atp5a1, by Santa Cruz Biotechnology (2 mentions) Atp5b, by Santa Cruz Biotechnology (2 mentions) Atp5d, by Abcam (2 mentions) Atp5o, by Abcam (2 mentions) Phos s6k ser235 236, by Cell Signaling Technology (2 mentions) Gata2, by Abcam (2 mentions) H3k56ac, by Cell Signaling Technology (2 mentions) H4k5ac, by Abcam (2 mentions) H3k36me3, by Cell Signaling Technology (2 mentions) H3k9me3, by Cell Signaling Technology (2 mentions) H3k27me3, by Cell Signaling Technology (2 mentions) 4e bp1, by Cell Signaling Technology (2 mentions) H3k4me3, by Cell Signaling Technology (2 mentions) H3k9ac, by Cell Signaling Technology (2 mentions) P70s6k, by Cell Signaling Technology (2 mentions) Gapdh, by Santa Cruz Biotechnology (2 mentions)

2 protocols using phos 4ebp1 thr37 46

1

Western Blot Analysis of Mitochondrial and Epigenetic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot was performed as described2 (link) using the following antibodies: TFAM (7495S, Cell Signaling Technology; sc-23588, Santa Cruz Technology), PHB2 (14085S, Cell Signaling Technology), ATP5A1 (sc-136178, Santa Cruz Technology), ATP5B (sc-16690, Santa Cruz Technology), ATP5D (ab97491, Abcam), ATP5O (ab110276, Abcam, clone 4C11C10D12), 4EBP1 (9644S, Cell Signaling Technology), phos-4EBP1 (Thr37/46) (2855S, Cell Signaling Technology), p70S6K (2708S, Cell Signaling Technology), phos-S6K (Ser235/236) (2211S, Cell Signaling Technology), GATA1 (ab47490, Abcam), GATA2 (ab22849, Abcam), TSC1 (6935, Cell Signaling Technology), TSC2 (4308, Cell Signaling Technology), H3K9ac (9649, Cell Signaling Technology), H3K27ac (8173, Cell Signaling Technology), H3K56ac (4243, Cell Signaling Technology), H4K5ac (ab51997, Abcam), H3K4me3 (9571, Cell Signaling Technology), H3K9me3 (13969, Cell Signaling Technology), H3K27me3 (9733, Cell Signaling Technology), H3K36me3 (4909, Cell Signaling Technology), H3 (4499, Cell Signaling Technology), ACTB (MAB1501, Millipore, clone C4), and GAPDH (sc-26778, Santa Cruz Biotechnology). Densitometry quantification was performed using ImageJ software. All antibodies were used at 1:1,000 dilutions except phos-4EBP1 (1:500), phos-S6K (1:500) and ACTB (1:2,500).
Liu X., Zhang Y., Ni M., Cao H., Signer R.A., Li D., Li M., Gu Z., Hu Z., Dickerson K.E., Weinberg S.E., Chandel N.S., DeBerardinis R.J., Zhou F., Shao Z, & Xu J. (2017). Regulation of Mitochondrial Biogenesis in Erythropoiesis by mTORC1-Mediated Protein Translation. Nature cell biology, 19(6), 626-638.
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2

Western Blot Analysis of Mitochondrial and Epigenetic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot was performed as described2 (link) using the following antibodies: TFAM (7495S, Cell Signaling Technology; sc-23588, Santa Cruz Technology), PHB2 (14085S, Cell Signaling Technology), ATP5A1 (sc-136178, Santa Cruz Technology), ATP5B (sc-16690, Santa Cruz Technology), ATP5D (ab97491, Abcam), ATP5O (ab110276, Abcam, clone 4C11C10D12), 4EBP1 (9644S, Cell Signaling Technology), phos-4EBP1 (Thr37/46) (2855S, Cell Signaling Technology), p70S6K (2708S, Cell Signaling Technology), phos-S6K (Ser235/236) (2211S, Cell Signaling Technology), GATA1 (ab47490, Abcam), GATA2 (ab22849, Abcam), TSC1 (6935, Cell Signaling Technology), TSC2 (4308, Cell Signaling Technology), H3K9ac (9649, Cell Signaling Technology), H3K27ac (8173, Cell Signaling Technology), H3K56ac (4243, Cell Signaling Technology), H4K5ac (ab51997, Abcam), H3K4me3 (9571, Cell Signaling Technology), H3K9me3 (13969, Cell Signaling Technology), H3K27me3 (9733, Cell Signaling Technology), H3K36me3 (4909, Cell Signaling Technology), H3 (4499, Cell Signaling Technology), ACTB (MAB1501, Millipore, clone C4), and GAPDH (sc-26778, Santa Cruz Biotechnology). Densitometry quantification was performed using ImageJ software. All antibodies were used at 1:1,000 dilutions except phos-4EBP1 (1:500), phos-S6K (1:500) and ACTB (1:2,500).
Liu X., Zhang Y., Ni M., Cao H., Signer R.A., Li D., Li M., Gu Z., Hu Z., Dickerson K.E., Weinberg S.E., Chandel N.S., DeBerardinis R.J., Zhou F., Shao Z, & Xu J. (2017). Regulation of Mitochondrial Biogenesis in Erythropoiesis by mTORC1-Mediated Protein Translation. Nature cell biology, 19(6), 626-638.
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