Propidium iodide pe

Manufactured by Merck Group

Propidium iodide-PE is a fluorescent dye used in flow cytometry applications. It binds to DNA and emits light in the PE (phycoerythrin) detection channel. This dye is commonly used to detect cell viability and DNA content.

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Lab products found in correlation

Annexin 5 fitc, by Merck Group (1 mentions) Annexin 5 fitc, by Abcam (1 mentions) Fortessa lsr x20, by BD (1 mentions)

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Propidium iodide (7211 citations)

2 protocols using propidium iodide pe

Apoptosis and Necrosis Evaluation

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Apoptosis and necrosis were assessed after 24 h of high glucose exposure by flow cytometry analysis of Annexin-V-FITC and propidium iodide-PE staining, respectively (Sigma-Aldrich). Caspase 3/7 activation and DNA fragmentation by TUNEL assay were assessed using commercially available kits (Promega).

Dunn L.L., Simpson P.J., Prosser H.C., Lecce L., Yuen G.S., Buckle A., Sieveking D.P., Vanags L.Z., Lim P.R., Chow R.W., Lam Y.T., Clayton Z., Bao S., Davies M.J., Stadler N., Celermajer D.S., Stocker R., Bursill C.A., Cooke J.P, & Ng M.K. (2014). A Critical Role for Thioredoxin-Interacting Protein in Diabetes-Related Impairment of Angiogenesis. Diabetes, 63(2), 675-687.

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Cell Viability and Apoptosis Assay

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Cells were treated with drug(s) for 72 hours in a 96-well flat-bottomed, clear microplate. Cell growth and viability was determined by the Alamar Blue assay as per the manufacturers instructions or the sulforhodamine-B (SRB) assay as previously described [44 (link)]. Cell viability and apoptosis was measured by staining cells with Annexin V-FITC (BioVision, Milpitas, CA) and propidium iodide-PE (Sigma-Aldrich, St. Louis, MO) as per manufacturer's instructions. All flow cytometry experiments were carried out using Canto II 96w or Fortessa LSR X20 cytometers (BD Biosciences, San Jose, CA). Flow cytometry data were analyzed with FlowJo version 7.6.5 (TreeStar, Ashland, OR).

Rotin L.E., Gronda M., MacLean N., Hurren R., Wang X., Lin F.H., Wrana J., Datti A., Barber D.L., Minden M.D., Slassi M, & Schimmer A.D. (2015). Ibrutinib synergizes with poly(ADP-ribose) glycohydrolase inhibitors to induce cell death in AML cells via a BTK-independent mechanism. Oncotarget, 7(3), 2765-2779.

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