TEL–SYK mice and GFP-control mice were injected with 100 μl/20 g body weight of 10 mM EdU (Click-iT EdU Pacific Blue Flow Cytometry Assay Kit, Molecular Probes, Eugene, OR, USA). BMCs were harvested 15 h later and LT-HSCs (Lin−cKit+Sca1+CD135−CD150+), ST-HSCs (Lin−cKit+Sca1+CD135−CD150−), MPPs (Lin−cKit+Sca1+CD135+CD150−) and myeloid progenitors (MPs) (Lin−cKit+Sca1−) were sorted with the ARIA III cell sorter (BD Biosciences). Samples were prepared according to the manufacturer's instructions and were analyzed for EdU+ cells by flow cytometry (Beckman Coulter). For cell cycle analysis of LKS cells in the BM, we used FxCycleT Violet stain (Molecular Probes) to stain DNA in combination with Lin(CD11b/Gr-1/CD3/CD19/Ter119)-PeCy7, cKit-APC, Sca1-APCCy7, CD135-PECy5 and CD150-PE (Biolegend) antibodies.
Cd135 pecy5
Manufactured by BioLegend
CD135-PECy5 is a fluorescently-labeled antibody that recognizes the CD135 (Flt3/Flk2) cell surface antigen. CD135 is a tyrosine kinase receptor that plays a role in hematopoietic stem cell development and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
C kit apc, by BioLegend (2 mentions)
Pe cd150, by BioLegend (1 mentions)
Aria 3 cell sorter, by BD (1 mentions)
Cd34 fitc, by Thermo Fisher Scientific (1 mentions)
Cd4 pe, by BioLegend (1 mentions)
B220 apc, by BioLegend (1 mentions)
Cd150 pe cy7, by BioLegend (1 mentions)
Cd8a pe, by BioLegend (1 mentions)
Cd11b apc, by BioLegend (1 mentions)
Ckit pe cy7, by BioLegend (1 mentions)
Cd48 apc cy7, by BioLegend (1 mentions)
Gr 1 pe, by BioLegend (1 mentions)
Sca 1 percp cy5, by Thermo Fisher Scientific (1 mentions)
Cd45.2 fitc, by Thermo Fisher Scientific (1 mentions)
C kit fitc, by BioLegend (1 mentions)
Ckit bv421, by BioLegend (1 mentions)
Sca 1 bv605, by BioLegend (1 mentions)
Sca 1 bv421, by BioLegend (1 mentions)
Cd150 bv421, by BioLegend (1 mentions)
Cd150 pe, by Thermo Fisher Scientific (1 mentions)
2 protocols using cd135 pecy5
1
Profiling Hematopoietic Stem Cell Kinetics
2
Multicolor Flow Cytometry Panel
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The following antibodies were used: mouse lineage cocktail-PE (BioLegend, cat# 78035), mouse lineage cocktail-APC (R&D Systems, cat# FLC001A), c-Kit-FITC (BioLegend, cat# 161603), c-Kit-APC (BioLegend, cat# 135108), c-Kit-PE/Cy7 (BioLegend, cat# 105814), c-Kit-BV421 (BioLegend, cat# 135124), Sca-1-BV605 (BioLegend, cat# 108133), Sca-1-APC (eBioscience, cat# 17-5981-82), Sca-1-BV421 (BioLegend, cat# 108127), Sca-1-PerCP-Cy5.5 (eBioscience, cat# 45-5981-82), CD150-BV421 (BioLegend, cat# 115925), CD150-PE (eBioscience, cat# 12-1501-82), CD150-PE-Cy7 (BioLegend, cat# 115913), CD48-PE/Cy7 (eBioscience, cat# 25-0481-80), CD48-BV711 (BioLegend, cat# 103439), CD48-APC-Cy7 (BioLegend, cat# 103432),CD34-FITC (eBioscience, cat# 11-0341-82), CD135-PE-Cy5 (BioLegend, cat# 135311), CD135-APC (BioLegend, cat# 135310), CD45.1-PerCP-Cy5.5 (BioLegend, cat# 110727), CD45.2-BV421 (BioLegend, cat# 109831), CD45.2-FITC(eBioscience, cat# 11-0454-82), Gr1-PE (BioLegend, cat# 108407), CD11b-APC (BioLegend cat# 101211), CD4-PE (BioLegend,cat# 116005), CD8a-PE (BioLegend, cat# 100707), B220-APC (BioLegend, cat# 103211).
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