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Horseradish peroxidase hrp conjugated secondary antibodies

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Horseradish peroxidase (HRP)-conjugated secondary antibodies are a type of laboratory reagent used in various immunoassay techniques. They are composed of a secondary antibody covalently linked to the enzyme horseradish peroxidase. These conjugated antibodies function as detection reagents, allowing the visualization and quantification of target analytes in samples.

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Lab products found in correlation

Ki67 antibody, by Abcam (2 mentions) Anti nup62 610497, by BD (2 mentions) Anti oga hpa036141, by Merck Group (2 mentions) Anti ha tag alexa fluor 647 conjugate, by Cell Signaling Technology (2 mentions) Nucblue fixed cell stain readyprobes reagent, by Thermo Fisher Scientific (2 mentions) Anti o glcnac rl2 ab2739, by Abcam (2 mentions) Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Anti flag f3165, by Merck Group (2 mentions) Irdye secondary antibody, by LI COR (2 mentions) Anti β actin sc 47778, by Santa Cruz Biotechnology (2 mentions) Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Cd31 antibody, by BD (1 mentions) Irdye800 700, by Rockland Immunochemicals (1 mentions) Phosphorylated s6 ser235 236, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) β actin antibody, by Merck Group (1 mentions)

Spelling variants of this product

Peroxidase-conjugated secondary antibodies (4 citations) Horseradish peroxidase (3 citations)

4 protocols using horseradish peroxidase hrp conjugated secondary antibodies

1

Immunohistochemical Analysis of EphB Signaling

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Media and fetal bovine serum were from Invitrogen (Carlsbad, CA). Monoclonal EphB4 antibodies used in immunohistochemistry and immunoprecipitation (Clone #131) and Western blotting (Clone #265) were from VasGene Inc. (Los Angeles, CA). EphB2 antibody was from R&D systems (Minneapolis, MN). Phospho-Tyrosine antibody (Clone 4G10) was from Millipore (Billerica, MA). CD31 antibody was from BD Biosciences (San Jose, CA). Ki67 antibody was from Abcam (Cambridge, MA). Phosphorylated S6 (Ser235/236) antibody was from Cell Signaling (Danvers, MA). Horse radish peroxidase (HRP) conjugated secondary antibodies were from Rockland (Gilbertsville, PA).
Li X., Choi W.W., Yan R., Yu H., Krasnoperov V., Kumar S.R., Schuckman A., Klumpp D.J., Pan C.X., Quinn D., Gill I.S., Gill P.S, & Liu R. (2014). The Differential Expression of EphB2 and EphB4 Receptor Kinases in Normal Bladder and in Transitional Cell Carcinoma of the Bladder. PLoS ONE, 9(8), e105326.
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2

Antibody Profiling of Axl, Mer, and Apoptosis Markers

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Antibodies against human Axl, Mer, cleaved caspase 3, Akt, phosphorylated Akt (Ser473), STAT3, phosphorylated STAT3 (Tyr705), Erk1/2, phosphorylated Erk1/2 (Thr202/Tyr204), ribosomal protein S6, and phosphorylated S6 (Ser235/236) were from Cell Signaling (Danvers, MA, USA). The Tyro3 and Gas6 antibodies were from R&D Systems (Minneapolis, MN, USA). β-Actin antibody was from Sigma-Aldrich (St Louis, MO, USA). Ki67 antibody was from Abcam (Cambridge, MA, USA). IRdye800/700 and horseradish peroxidase (HRP)-conjugated secondary antibodies were from Rockland Immunochemicals (Gilbertsville, PA, USA). The TdT-mediated dUTP nick-end labelling (TUNEL) assay kit was from Promega (Madison, MI, USA). Protease inhibitor cocktail was from Thermo Scientific (Waltham, MA, USA).
Yu H., Liu R., Ma B., Li X., Yen H.Y., Zhou Y., Krasnoperov V., Xia Z., Zhang X., Bove A.M., Buscarini M., Parekh D., Gill I.S., Liao Q., Tretiakova M., Quinn D., Zhao J, & Gill P.S. (2015). Axl receptor tyrosine kinase is a potential therapeutic target in renal cell carcinoma. British Journal of Cancer, 113(4), 616-625.
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3

Antibody Procurement for Protein Detection

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Anti-Flag (F3165) and anti-OGA (HPA036141) were purchased from Sigma-Aldrich. Anti-DYKDDDK Tag (14793), anti-myc (2276), anti-HA (3724), anti-GAPDH (5174), anti-OGT (24083), anti-His-Tag (12698), anti-CREB (9197), anti-c-Jun (60A8), anti-c-Fos (2250) and anti-HA-Tag (Alexa Fluor® 647 Conjugate) (3444) were purchased from Cell Signaling Technology. Anti-O-GlcNAc(RL2) (ab2739) was obtained from Abcam. Anti-Nup62 (610497) was purchased from BD Biosciences. Anti-β-actin (sc-47778) was purchased from Santa Cruz Biotechnology. Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Rockland Immunochemicals. IRDye® secondary antibodies were purchased from LI-COR Biosciences. Alexa Fluor™ 488 anti-rabbit IgG (A11008), Alexa Fluor™ 647 anti-rabbit IgG (A21244), Alex Fluor™ 568 anti-mouse IgG (A11004) and NucBlue™ Fixed Cell Stain ReadyProbes™ reagent (R37606) were purchased from Invitrogen. OSMI-4b, Ac45SGlcNAc and Biotin-Alkyne probe were homemade.
Ge Y., Ramirez D.H., Yang B., D’Souza A.K., Aonbangkhen C., Wong S, & Woo C.M. (2021). Target protein deglycosylation in living cells by a nanobody-fused split O-GlcNAcase. Nature chemical biology, 17(5), 593-600.
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4

Antibody Procurement for Protein Detection

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Anti-Flag (F3165) and anti-OGA (HPA036141) were purchased from Sigma-Aldrich. Anti-DYKDDDK Tag (14793), anti-myc (2276), anti-HA (3724), anti-GAPDH (5174), anti-OGT (24083), anti-His-Tag (12698), anti-CREB (9197), anti-c-Jun (60A8), anti-c-Fos (2250) and anti-HA-Tag (Alexa Fluor® 647 Conjugate) (3444) were purchased from Cell Signaling Technology. Anti-O-GlcNAc(RL2) (ab2739) was obtained from Abcam. Anti-Nup62 (610497) was purchased from BD Biosciences. Anti-β-actin (sc-47778) was purchased from Santa Cruz Biotechnology. Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Rockland Immunochemicals. IRDye® secondary antibodies were purchased from LI-COR Biosciences. Alexa Fluor™ 488 anti-rabbit IgG (A11008), Alexa Fluor™ 647 anti-rabbit IgG (A21244), Alex Fluor™ 568 anti-mouse IgG (A11004) and NucBlue™ Fixed Cell Stain ReadyProbes™ reagent (R37606) were purchased from Invitrogen. OSMI-4b, Ac45SGlcNAc and Biotin-Alkyne probe were homemade.
Ge Y., Ramirez D.H., Yang B., D’Souza A.K., Aonbangkhen C., Wong S, & Woo C.M. (2021). Target protein deglycosylation in living cells by a nanobody-fused split O-GlcNAcase. Nature chemical biology, 17(5), 593-600.
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