90i upright microscope

Manufactured by Nikon

The Nikon 90i upright microscope is a high-performance laboratory instrument designed for a wide range of scientific applications. It features a sturdy and stable construction, providing a reliable platform for precise observation and analysis. The microscope offers advanced optics, including high-quality lenses and illumination systems, to deliver clear and detailed images. Its versatile design accommodates a variety of sample types and magnification ranges, making it suitable for various research and examination tasks in fields such as biology, materials science, and more.

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Lab products found in correlation

1.4 na objective, by Nikon (2 mentions) Plan apochromat, by Nikon (1 mentions) Bright field microscope, by Nikon (1 mentions) Fluoview 1000 confocal microscope, by Olympus (1 mentions) Agarose, by Thermo Fisher Scientific (1 mentions) Nb100 304, by Novus Biologicals (1 mentions) Rnascope multiplex fluorescent reagent kit version 2, by Advanced Cell Diagnostics (1 mentions) Orca flash 4 camera, by Hamamatsu Photonics (1 mentions) Ti e inverted microscope, by Nikon (1 mentions) Nis element, by Nikon (1 mentions)

Close spelling variants of this product

ECLIPSE 90i upright microscope Nikon 90i Upright Widefield Microscope Nikon 90i upright fluorescence microscope Nikon 90i microscope Upright microscope

10 protocols using 90i upright microscope

Confocal Imaging of Whole-Mount Embryos

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Confocal imaging of whole-mount embryo specimens was performed using a Nikon 90i upright microscope with a Nikon C1 confocal scan head and Nikon Plan Apochromat 10x and 20x objectives.

Aleksandrova A., Czirok A., Kosa E., Galkin O., Cheuvront T.J, & Rongish B.J. (2015). The endoderm and myocardium join forces to drive early heart tube assembly. Developmental biology, 404(1), 40-54.

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Immunostaining of XIAP in Biliary Ducts and CCAs

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Tissue immunostaining for XIAP, in FFPE sections of normal biliary ducts and CCAs was performed as previously described [20 (link)]. XIAP (#2945, Lifespan Biosciences, Inc.) antibody was diluted in TBS-T-goat serum and incubated overnight at 4 °C. Sections were stained with DAB Peroxidase Substrate Kit and counterstained with hematoxylin QS. Images were captured with a Nikon 90i Upright Microscope equipped with a Nikon Digital camera.

Palumbo T., Poultsides G.A., Kouraklis G., Liakakos T., Drakaki A., Peros G., Hatziapostolou M, & Iliopoulos D. (2016). A functional microRNA library screen reveals miR-410 as a novel anti-apoptotic regulator of cholangiocarcinoma. BMC Cancer, 16, 353.

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Multimodal Microscopic Imaging of Biological Samples

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Samples were imaged with Olympus Fluoview 1000 confocal microscope using a 60x oil objective. Immunohistochemistry images were represented as z projections of confocal stacks acquired from serial laser scanning. Images of overall myelin expression were acquired with a Nikon 90i upright microscope using a 20x objective and modified using NIS-Elements software to show only red channel (myelin expression). Muscle images were acquired with a Nikon brightfield microscope.

Kostereva N.V., Wang Y., Fletcher D.R., Unadkat J.V., Schnider J.T., Komatsu C., Yang Y., Stolz D.B., Davis M.R., Plock J.A, & Gorantla V.S. (2016). IGF-1 and Chondroitinase ABC Augment Nerve Regeneration after Vascularized Composite Limb Allotransplantation. PLoS ONE, 11(6), e0156149.

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Imaging Intracellular Protein Aggregates

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Images were taken with a Nikon 90i upright microscope equipped with a Nikon Plan Apo 100X/1.4 phase-contrast objective. Images were collected with a Rolera XR cooled CCD camera and initially processed by NIS-Elements Advanced Research software. Images were further analyzed for inclusion-body content with either custom Matlab code or ImageJ. Samples were spotted onto 1% agarose (Invitrogen) pads, resting on glass slides, made with the appropriate medium. Coverslips were sealed with valap (1:1:1, lanolin:paraffin:petroleum jelly).

Castellana M., Wilson M.Z., Xu Y., Joshi P., Cristea I.M., Rabinowitz J.D., Gitai Z, & Wingreen N.S. (2014). Enzyme clustering accelerates processing of intermediates through metabolic channeling. Nature biotechnology, 32(10), 1011-1018.

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Embryonic Brain Histology Protocol

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Whole heads from embryos at E17.5 were sectioned sagitally at 7μm. Sections were then stained with hematoxylin and eosin (H&E). Briefly, for depariffinization and rehydration, sections were passed through three changes of xylene followed by washes through reducing grades of ethanol. Slides were then incubated in Gill’s Hematoxylin No. III (Sigma-Aldrich, St. Louis, MO) for 1.5 minutes, washed briefly in tap water, and incubated in a 1% Eosin solution (Eosin Y-VWR, West Chester, PA) for 1.5 minutes. To dehydrate, slides were passed through increasing grades of ethanol. Lastly, slides were mounted using Permount mounting media (VWR, Radnor, PA). Stained sections were imaged using a Nikon 90i upright microscope.

Smith A.L., Kousa Y.A., Kinoshita A., Fodor K., Yang B, & Schutte B.C. (2017). Generation and Characterization of a Conditional Allele of Interferon regulatory factor 6. Genesis (New York, N.Y. : 2000), 55(7), 10.1002/dvg.23038.

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Visualizing antibiotic-induced changes in E. coli

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Following the protocol of Nonejuie et al. (2013) (link), overnight E. coli lptD4213 cultures were diluted 1:100 in LB and grown at 30°C for 90 minutes on a roller drum. Each culture was then treated with 5X the MIC and incubated at 30°C with slight agitation. Following antibiotic treatment, cells were stained with 0.5 μM SYTOX Green, 1 μg/mL FM4–64, and 2 μg/mL DAPI for incubated for 10 minutes. Each stained culture was then centrifuged at 3220 rcf. for 40 s and resuspended in 1/10 volume of LB. Cells spotted onto a 1.2% agarose pad in 20% LB medium for imaging. Images were collected on a Nikon 90i upright microscope equipped with a 100X 1.4 N.A. objective (Nikon Instruments Inc., Melville, NY) and a RoleraXR (Photometrics, Tucson, AZ) camera. Microscope control and image acquisition were performed in NIS Elements.

Martin JK I.I., Sheehan J.P., Bratton B.P., Moore G.M., Mateus A., Li S.H., Kim H., Rabinowitz J.D., Typas A., Savitski M.M., Wilson M.Z, & Gitai Z. (2020). A Dual-Mechanism Antibiotic Kills Gram-Negative Bacteria and Avoids Drug Resistance. Cell, 181(7), 1518-1532.e14.

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Histological Examination of TEVG Explants

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Explant segments intended for histological staining were sliced into 5 μm sections and stained for cell and elastin distribution using H&E and Verhoeff van Gieson (VVG) staining, respectively. Staining was performed by the Histology Core at the McGowan Institute for Regenerative Medicine. Stained sections were imaged using a Nikon 90i upright microscope. Prior to sectioning the explant for histological examination, the carotid artery tissue was separated from the TEVG along the anastomosis to ensure that sections L1–L7 reflect the structure of the explanted graft.

Cunnane E.M., Lorentz K.L., Soletti L., Ramaswamy A.K., Chung T.K., Haskett D.G., Luketich S.K., Tzeng E., D’Amore A., Wagner W.R., Weinbaum J.S, & Vorp D.A. (2020). Development of a Semi-Automated, Bulk Seeding Device for Large Animal Model Implantation of Tissue Engineered Vascular Grafts. Frontiers in Bioengineering and Biotechnology, 8, 597847.

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DNA Damage Response Imaging Assay

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The cells were plated on coverslips, irradiated with 5 Gy if indicated, and collected at varying time intervals. At collection, the cells were fixed with 4% paraformaldehyde and 0.2% Triton X-100 in PBS for 20 min at RT, followed by 3 washes in PBS. After blocking in 1% BSA and 0.1% Triton X-100 in PBS for 1 h at 37 °C, the cells were incubated with 53BP1 (NB100-304, Novus Biologicals), or Anti-phospho-Histone H2AX (Ser139) clone JBW301(05-636, Millipore) antibodies (1:3000 dilution), overnight at 37 °C. Three washes in PBS were carried out followed by incubation for 1 h at 37 °C with the appropriate secondary antibodies. The final washes in PBS were carried out, the cells were counterstained with DAPI and coverslips were mounted on slides using gervatol mounting medium. Fluorescent micrographs were taken using a Nikon 90i upright microscope. These experiments were performed in triplicate.

, & Ortega-Martínez S. (2015). Dexamethasone acts as a radiosensitizer in three astrocytoma cell lines via oxidative stress. Redox Biology, 5, 388-397.

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Transcriptomic Analysis of Placental Development

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Transcripts for TFAP2C, EPAS1, SNAI1, PLAC8, and CDH1 were localized within human first trimester placental tissue sections. RNAscope Multiplex Fluorescent Reagent Kit version 2 (Advanced Cell Diagnostics) was used for in situ hybridization analysis. Probes were prepared to detect TFAP2C (NM_003222.3, 515921, target region: 664–1596), EPAS1 (NM_001430.4, 410591, target region: 1332–2354), SNAI1 (NM_005985.3, 560421, target region: 17−1233), CDH1 (NM_004360.3, 311091-C2, target region: 263–1255), and PLAC8 (NM_016619.3, 858491-C2, target region: 5–1448). CDH1 and PLAC8 probes were used to identify cytotrophoblast/trophoblast progenitor cells and differentiated EVT cells of the EVT cell column, respectively^{48 (link),49}. Fluorescence images were captured on a Nikon 90i upright microscope (Nikon) with a Photometrics CoolSNAP-ES monochrome camera (Roper).

Varberg K.M., Dominguez E.M., Koseva B., Varberg J.M., McNally R.P., Moreno-Irusta A., Wesley E.R., Iqbal K., Cheung W.A., Schwendinger-Schreck C., Smail C., Okae H., Arima T., Lydic M., Holoch K., Marsh C., Soares M.J, & Grundberg E. (2023). Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape. Nature Communications, 14, 4826.

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Microscopic Visualization of Antibiotic-Treated E. coli

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In dual-treatment experiments (Figures 6A and S6), overnight E. coli lptD4213 cultures were diluted 1:100 and grown to early-mid exponential phase (OD₆₀₀ = 0.4–0.6). Each culture was then diluted 1:10 into fresh LB and treated with the desired concentration of antibiotic for 10 minutes. Following antibiotic treatment, cells were stained with 0.5 μM SYTOX Green, 1 μg/mL FM4–64, and 2 μg/mL DAPI. Each stained culture was then spotted onto a 1.5% agar pad supplemented with casamino acids and glucose in M63 (15 mM (NH₄)₂SO₄, 100 mM KH₂PO₄, 1.7 μM FeSO₄, 0.5% glucose, 0.2% casamino acids, 1 mM MgSO₄). Images were collected on either Nikon 90i upright microscope equipped with a 100X 1.4 N.A. objective (Nikon) or a Nikon Ti-E inverted microscope equipped with a 100X 1.4 NA objective. Both microscopes utilize an Orca Flash4 camera (Hamamatsu, Bridgewater, NJ). Microscope control and image acquisition were performed in NIS Elements (Nikon).

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