The hepatitis B surface antigen (HBsAg) enhanced chemiluminescence detection kit was purchased from Roche Diagnostics. RNA extraction kit (cat. no. AP-MN-MS-RNA-250) was from Axygen; Corning Inc., and reverse transcription (RT) kit was purchased from Takara Biotechnology Co., Ltd. (cat. no. RR037A). Mouse anti-human BDCA-2 (cat. no. 748001) and ILT7 monoclonal antibodies (cat. no. 562500) were purchased from BD Pharmigen; BD Biosciences. Goat anti-mouse biotin-labeled secondary antibody (cat. no. 31800), diaminobenzidine (DAB) chromogenic reagent and immunohistochemistry (IHC) streptavidin peroxidase conjugated method (SP) kit were purchased from Zymed (Thermo Fisher Scientific, Inc.). The Labsystem Version 1.3.1 microplate reader was purchased from Bio-Rad Laboratories, Inc. The ABI 7700 Fast Quantitative PCR Reactor was from ABI (Thermo Fisher Scientific, Inc.). The Cobas c311 automatic biochemical analyzer was purchased from Roche Diagnostics. The C6015-2 B-ultrasonic instrument was purchased from SonoSite, Inc.
Labsystem version 1.3.1 microplate reader
Manufactured by Bio-Rad
Sourced in United States
The Labsystem Version 1.3.1 microplate reader is a laboratory instrument designed to measure the absorbance or fluorescence of samples in a microplate format. It can perform various types of assays, including colorimetric, fluorometric, and luminometric measurements.
Automatically generated - may contain errors
Lab products found in correlation
Ecl reagent, by Cytiva (4 mentions)
Pvdf membrane, by Pall Corporation (4 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (3 mentions)
Western blot related chemical reagents, by Beyotime (3 mentions)
Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (2 mentions)
Hrp tagged igg secondary antibody, by Cell Signaling Technology (2 mentions)
Cobas c311 automatic biochemical analyzer, by Roche (1 mentions)
Nilotinib, by Merck Group (1 mentions)
Mmp 2, by Merck Group (1 mentions)
Mmp 9 elisa kit, by R&D Systems (1 mentions)
Transwell chamber, by Merck Group (1 mentions)
Trypsin edta, by Merck Group (1 mentions)
Glutamine, by Merck Group (1 mentions)
Brain derived neurotrophic factor (bdnf), by Merck Group (1 mentions)
Western blotting related chemical reagents, by Beyotime (1 mentions)
Lipo2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Rgc 5 cells, by American Type Culture Collection (1 mentions)
Hmgb1 elisa kit, by R&D Systems (1 mentions)
Phosphorylated pi3k, by Cell Signaling Technology (1 mentions)
Mtt powder, by Thermo Fisher Scientific (1 mentions)
7 protocols using labsystem version 1.3.1 microplate reader
1
HBsAg Detection and BDCA-2/ILT7 Immunohistochemistry
2
Comprehensive Cell Culture Protocol
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DMEM medium, fetal bovine serum (FBS), EDTA, and penicillin-streptomycin were purchased from Hyclone; dimethyl sulfoxide and MTT were purchased from Gibco; trypsin-EDTA was purchased from Sigma; PVDF membrane was purchased from Pall Life Sciences; Western blot-related chemical reagents were from Shanghai Beyotime biotechnology Co. LTD; ECL reagent was purchased from Amersham Biosciences; DDR-1 monoclonal antibody and HRP tagged IgG secondary antibody were purchased from Cell Signaling; nilotinib and transwell chambers were purchased from Sigma; MMP-2 and MMP-9 ELISA kits were purchased from R&D; other reagents were purchased from Shanghai Sangon Biotechnology Co., LTD; and Labsystem Version1.3.1 microplate reader was purchased from Bio-rad.
3
RGC-5 Cells Culture Protocol
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RGC-5 cells were bought from the ATCC cell bank. DMEM, EDTA, and penicillin-streptomycin were obtained from Hyclone. B27, CNTF, BDNF, enzyme, and glutamine were purchased from Sigma. Dimethyl sulfoxide and MTT were purchased from Gibco. PVDF membrane was obtained from Pall Life Sciences. Lipo2000 transfection reagent was bought from Invitrogen. Western blotting-related chemical reagents were purchased from Shanghai Beyotime Biotechnology Co., LTD. ECL reagent was obtained from Amersham Biosciences. TLR4 primary antibody and secondary antibody were obtained from Cell Signaling. HMGB-1 ELISA kit was purchased from R&D. Other reagents were purchased from Shanghai Sangon Biotechnology Co., LTD. Labsystem Version1.3.1 microplate reader was bought from Bio-Rad.
4
Chemoresistance Mechanisms in A549 Cells
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A549 and A549/DDP cell lines were purchased from the ATCC Cell Bank (USA). DMEM medium, fetal bovine serum (FBS), EDTA, and penicillin-streptomycin were bought from Hyclone (USA). Dimethyl sulfoxide and MTT powder were from Gibco. Tyresin-EDTA was from Sigma (USA). PVDF membrane was from Pall Life Sciences. Western blot-related chemical reagents were obtained from Beyotime. ECL reagent was from Amersham Biosciences. ERCC1, BRCA1, PI3K, AKT, phosphorylated PI3K, and phosphorylated AKT monoclonal antibodies, and HRP-tagged IgG secondary antibody were from Cell Signaling. Caspase 3 activity kits were purchased from Pall Life Sciences. RNA extraction kits and reverse transcription kits were from Axygen (USA). Other common reagents were purchased from Sangon. The Labsystem version 1.3.1 microplate reader was purchased from Bio-Rad (USA).
5
Hydrochloric Acid for Photodynamic Therapy
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Hydrochloric acid ammonium was purchased from Fudan Zhangjiang Pharmaceutical (China approval H20070027). Photodynamic therapy instrument was provided by Boji (China). LabSystem Version 1.3.1 microplate reader was purchased from Bio-Rad (USA). Rat anti-human p53 monoclonal antibody and anti-biotin goat anti-mouse IgG secondary antibody were purchased from Boster (China). IHC SP kit and H&E staining kit were purchased from Boster (China). ELISA kits for TNF-α and IL-6 were purchased from R&D (USA). Other common reagents were purchased from Sangon (China).
6
Cytokine Detection Kit Assay
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Detection kits for human IL-6, IL-10, and MCP-1 were purchased from R&D Systems (Minneapolis, MN, USA). The Labsystem Version 1.3.1 microplate reader was purchased from Bio-Rad (Hercules, CA, USA). The Elecsys automated ECL analyzer was purchased from Roche (Basel, Switzerland).
7
MCF-7 Cell Cytotoxicity Assay Protocol
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MCF-7 cell line was bought from the cell bank of Chinese academy of sciences. LA, paclitaxel, and enzyme-EDTA were obtained from Sigma Aldrich (St. Louis, MO, USA). DMEM medium was purchased from Gibco (Carlsbad, CA, USA). Fetal bovine serum (FBS) and penicillinstreptomycin were from Hyclone (Logan, Utah, USA). PVDF membrane was bought from Pall Life Sciences (Port Washington, NY, USA). MTT reagents, dimethyl sulfoxide, and western blot-related chemical reagents were from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China). ECL reagent was obtained from Amersham Biosciences (Piscataway, NJ, USA). NF-κB primary antibody and HRP tag IgG second antibody were bought from Cell Signaling (Danvers, Massachusetts, USA). Other reagents were purchased from Shanghai Sangon Biotechnology Co., Ltd. (Shanghai, China). Labsystem Version 1.3.1 microplate reader was bought from Bio-rad (Hercules, CA, USA).
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