Bond automated stainer

Manufactured by Leica camera

The Bond automated stainer is a laboratory instrument designed to automate the process of biological sample staining. It provides a consistent and efficient way to prepare samples for microscopic analysis. The core function of the Bond automated stainer is to apply various stains and reagents to slides or other laboratory specimens in a controlled and reproducible manner.

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Lab products found in correlation

Ab8158, by Abcam (1 mentions) Axio imager m2 microscope, by Zeiss (1 mentions) Axiocam mrm rev 3, by Zeiss (1 mentions) Bond wash, by Leica camera (1 mentions) Bond polymer refine red detection kit, by Leica camera (1 mentions)

2 protocols using bond automated stainer

Immunohistochemical Analysis of Tumor Tissue

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For histological evaluation, harvested tumor tissue was fixed in 2% formalin (neutral buffered) and embedded in paraffin. Thin tissue slices (~5 µm) were cut using cryotome, and the sections were mounted for antigen retrieval. Standard immunohistochemistry steps of deparaffinizing and rehydrating with various solvents was followed, and the immunostaining (Leica Bond automated stainer) was carried out. Primary and secondary antibodies were used for CD34 (Abcam ab8158 / 1:100 and HRP) and phosphohistone gammaH2AX: ser139 staining (Cell signaling technologies; #20E3 / 1:400). Sections were stained and counterstained with Mayers hematoxylin. Following blocking and DAB steps, images were visualized using a Zeiss Axio Imager M2 microscope with a high-resolution Axiocam Mrm Rev.3 camera at 20x and 100x magnifications.

Kunjachan S., Kotb S., Pola R., Pechar M., Kumar R., Singh B., Gremse F., Taleeli R., Trichard F., Motto-Ros V., Sancey L., Detappe A., Yasmin-Karim S., Protti A., Shanmugam I., Ireland T., Etrych T., Sridhar S., Tillement O., Makrigiorgos M, & Berbeco R.I. (2019). RETRACTED ARTICLE: Selective Priming of Tumor Blood Vessels by Radiation Therapy Enhances Nanodrug Delivery. Scientific Reports, 9, 15844.

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PDL-1 Immunohistochemistry in Tumor Tissue

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Tumors were harvested and flash-frozen in liquid nitrogen. Frozen tissue sections were cut at 5 μm onto coated slides. Sections were air-dried overnight and then fixed in 10% neutral buffered formalin (NBF) for 5 min before being treated with 1% H₂O₂ in dH₂O for 15 min at room temperature. Slides were then washed in dH₂O to remove excess H₂O₂. Slides were rinsed in Bond Wash (Leica) and placed on the Leica Bond Automated stainer. The slides were stained with Rat primary PDL-1 (13-5982-52; EBio) 1:500 in Animal Free Diluent (SP-5035; Vector Labs). The BOND Polymer Refine Red Detection kit (Leica) was used according to the manufacturer’s protocol. The slides were then digitized according to a previously described protocol.⁵⁷ (link)

El-Sayes N., Walsh S., Vito A., Reihani A., Ask K., Wan Y, & Mossman K. (2022). IFNAR blockade synergizes with oncolytic VSV to prevent virus-mediated PD-L1 expression and promote antitumor T cell activity. Molecular Therapy Oncolytics, 25, 16-30.

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