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Vista analyzer

Manufactured by Siemens
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Sourced in Germany

The Vista analyzer is a laboratory equipment product designed to perform various analytical tasks. It is capable of analyzing samples and providing data to support research and testing activities. The Vista analyzer's core function is to facilitate efficient and accurate sample analysis, without making claims about its intended use or application.

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Lab products found in correlation

Dimension xpand, by Siemens (2 mentions) Cell dyn sapphire, by Abbott (1 mentions) Immulite 2500 analyzer, by Siemens (1 mentions) Vitros 950 analyzer, by Ortho Clinical Diagnostics (1 mentions) Xe 5000 analyzer, by Sysmex (1 mentions) Cobas 6000, by Roche (1 mentions) Cobas e411, by Roche (1 mentions) St5010 autostainer xl, by Leica camera (1 mentions) Leica asp300s tissue processor, by Leica Microsystems (1 mentions) Elisa, by ALPCO (1 mentions) Quantikine elisa, by R&D Systems (1 mentions) G8 hplc analyzer, by Tosoh (1 mentions) Immulite 2000 xpi analyzer, by Siemens (1 mentions)

11 protocols using vista analyzer

1

Extracellular Vesicle Isolation and Protein Analysis

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For total extracellular vesicles from 30 to 120 nm isolation from 738 serum samples (three samples T1, T2, and D for 246 women), we used a kit (ref 4478360) from InvitrogenTM (Carlsbad, California, United States) using 450 μl of serum and following the manufacturer’s instructions. Then, for extraction of total proteins from extracellular vesicles, we used InvitrogenTM kit (ref 4478545) following the manufacturer’s instructions. The assay of total proteins in the extracellular vesicle extracts was carried out using a Vista® analyzer (Siemens, Munich, Germany). The assay of apolipoprotein B (Apo B) in 16 extracellular vesicle extracts (eight from the control group and eight from the PPROM group) was carried out using a Vista® analyzer (Siemens, Munich, Germany).
Bouvier D., Giguère Y., Blanchon L., Bujold E., Pereira B., Bernard N., Gallot D., Sapin V, & Forest J.C. (2020). Study of sRAGE, HMGB1, AGE, and S100A8/A9 Concentrations in Plasma and in Serum-Extracted Extracellular Vesicles of Pregnant Women With Preterm Premature Rupture of Membranes. Frontiers in Physiology, 11, 609.
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2

Routine Lipid Profile Analysis

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Routine laboratory tests were performed by the Department of Laboratory Medicine in the NIH Clinical Center. Serum total cholesterol (TC), direct HDL-C and TG were measured enzymatically on a Siemens Vista Analyzer. Serum LDL-C was calculated using the Friedewald equation. Serum ApoA-I and ApoB were measured nephelometrically on a Siemens Vista Analyzer. % CE in plasma was determined by enzymatically measuring total cholesterol, as well as free cholesterol, in the absence of cholesteryl esterase.
Moraitis A.G., Freeman L.A., Shamburek R.D., Wesley R., Wilson W., Grant C.M., Price S., Demosky S., Thacker S.G., Zarzour A., Hornung R.L., Pucino F., Csako G., Yarboro C., McInnes I.B., Kuroiwa T., Boumpas D., Rao V.K., Illei G.G, & Remaley A.T. (2014). Elevated Interleukin-10: A New Cause of Dyslipidemia Leading to Severe HDL Deficiency: Moraitis: Elevated IL-10 and Severe HDL Deficiency. Journal of clinical lipidology, 9(1), 81-90.
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3

Comprehensive Metabolic Evaluation Protocol

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Patients underwent routine hematological, immunological, and biochemical testing after an overnight fast. Glucose and lipid values were determined by a Vista analyzer (Siemens Healthcare Diagnostics, Deerfield, IL). Insulin was analyzed by a chemiluminescence immunoassay on a Siemens Immulite 2500 analyzer. HbA1c was measured by high-performance liquid chromatography. Complete blood count and differential was determined by Cell-Dyn Sapphire (Abbott Diagnostics, Santa Clara, CA). Total testosterone was measured by chemiluminescence immunoassay on a Siemens Immulite 2500 analyzer. The presence of the insulin receptor antibody was confirmed by measuring the ability of patients’ sera to immunoprecipitate recombinant human insulin receptors, as described previously (9 (link)). Titers were based on semiquantitative assessment of Western blot band intensities compared with a positive control and were scored by one observer (R.K.S.).
Klubo-Gwiezdzinska J., Lange M., Cochran E., Semple R.K., Gewert C., Brown R.J, & Gorden P. (2018). Combined Immunosuppressive Therapy Induces Remission in Patients With Severe Type B Insulin Resistance: A Prospective Cohort Study. Diabetes Care, 41(11), 2353-2360.
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4

Cardiovascular Biomarkers in Admission

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Blood samples were drawn on admission. The median (interquartile range, IQR) time from symptom onset to blood sampling was 17(8–28) hours. Plasma N-terminal fragment of B-type natriuretic peptide (Nt-proBNP) was measured using homogeneous chemiluminescence immunoassay on a Vista analyzer (SIEMENS). Blood glucose was assessed by the enzymatic method on a Vitros 950 analyzer (Ortho Clinical Diagnostics, Rochester, NY). Plasma creatinine levels were measured on a Vitros 950 analyzer (Ortho Clinical Diagnostics, Rochester, NY). Glomerular Filtration Rate was calculated by CKD-EPI formula [21 ]. C-reactive protein (CRP) was determined on a dimension Xpand (Dade Behring, Deerfield, IL) using enzymatic methods. The CRP level was dichotomized at 3 mg/L for more clinical relevance.
Stamboul K., Lorin J., Lorgis L., Guenancia C., Beer J.C., Touzery C., Rochette L., Vergely C., Cottin Y, & Zeller M. (2015). Atrial Fibrillation Is Associated with a Marker of Endothelial Function and Oxidative Stress in Patients with Acute Myocardial Infarction. PLoS ONE, 10(7), e0131439.
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5

Plasma Lipid Profile Analysis

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Concentrations of plasma total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides were determined in samples having a suitable plasma volume (n = 7 for LFDc, n = 8 for LFDi, n = 6 for HFDc, and n = 7 for HFDi) to be analyzed using a Vista analyzer (Siemens Healthcare Diagnostics, Deerfield, IL, USA).
Albouery M., Bretin A., Buteau B., Grégoire S., Martine L., Gambert S., Bron A.M., Acar N., Chassaing B, & Bringer M.A. (2021). Soluble Fiber Inulin Consumption Limits Alterations of the Gut Microbiota and Hepatic Fatty Acid Metabolism Caused by High-Fat Diet. Nutrients, 13(3), 1037.
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6

Comprehensive Blood and Serum Biomarker Analysis

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Hemoglobin, hematocrit, mean corpuscular volume (MCV), absolute reticulocyte count, and percentage reticulocyte count were measured in EDTA-anticoagulated whole blood using a Sysmex XE-5000 analyzer (Chicago, IL). Glucose, total bilirubin, direct bilirubin, liver enzymes, blood urea nitrogen, creatinine, vitamin B12, and folate were measured in serum using the Vista analyzer (Siemens Healthcare, Malvern, PA). Insulin was measured in serum on the Cobas 6000 instrument (Roche Diagnostics, Indianapolis, IN). Iron, transferrin, and ferritin were analyzed in serum on the Immulite XP and analyzer (Siemens Healthcare), and urinary microalbumin was measured using the Dimension Xpand (Siemens Healthcare).
Sumner A.E., Thoreson C.K., O'Connor M.Y., Ricks M., Chung S.T., Tulloch-Reid M.K., Lozier J.N, & Sacks D.B. (2014). Detection of Abnormal Glucose Tolerance in Africans Is Improved by Combining A1C With Fasting Glucose: The Africans in America Study. Diabetes Care, 38(2), 213-219.
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7

Serum S100B and CK Analysis

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Venous blood samples were centrifuged at 2,100 g for 15 min and stored at −20°C until analysis. The S100B results did not influence patients' clinical management.
Serum S100B concentrations were determined by an electro-chemiluminescence immunoassay using a Roche Diagnostics Cobas e411® instrument (Meylan, France). The assay time was 18 min, the sample volume was 20 μL, and the lower detection limit was 0.005 μg/L. Concentrations of up to 39 μg/L could be measured without dilution. Typical within-assay precision was below 5%. The results are reported in micrograms per liter and rounded to two decimal places. Total CK activity concentration assays were performed using a Vista® analyzer (Siemens, Munich, Germany) following the manufacturer's recommendations.
Kahouadji S., Salamin P., Praz L., Coiffier J., Frochaux V., Durif J., Pereira B., Arlettaz L., Oris C., Sapin V, & Bouvier D. (2020). S100B Blood Level Determination for Early Management of Ski-Related Mild Traumatic Brain Injury: A Pilot Study. Frontiers in Neurology, 11, 856.
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8

Assessing Iron Deficiency in Inflammation

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An iron profile was determined in all patients, by measuring serum ferritin and soluble transferrin receptor (sTfR) using a Vista analyzer and NLatex sTfR, BNPprospec (Siemens, France). Iron deficiency was defined according to the usual definition in the presence of inflammation, by either ferritin < 100 ng/l or sTfR/log(ferritin) ratio > 0.8 [14 (link), 15 (link)].
Lasocki S., Lefebvre T., Mayeur C., Puy H., Mebazaa A, & Gayat E. (2018). Iron deficiency diagnosed using hepcidin on critical care discharge is an independent risk factor for death and poor quality of life at one year: an observational prospective study on 1161 patients. Critical Care, 22, 314.
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9

Quantification of Serum Liver Enzymes and IL-13Rα2 Levels

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Serum was analyzed for liver enzyme quantification at the National Institutes of Health Clinical Center using a Vista Analyzer (Siemens; Deerfield, IL). IL-13Rα2 serum levels were determined by ELISA as previously described [38] (link).
Vannella K.M., Barron L., Borthwick L.A., Kindrachuk K.N., Narasimhan P.B., Hart K.M., Thompson R.W., White S., Cheever A.W., Ramalingam T.R, & Wynn T.A. (2014). Incomplete Deletion of IL-4Rα by LysMCre Reveals Distinct Subsets of M2 Macrophages Controlling Inflammation and Fibrosis in Chronic Schistosomiasis. PLoS Pathogens, 10(9), e1004372.
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10

Prostate Tissue Handling and Characterization

Cited 1 time
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Normal tumor-adjacent prostate tissues from the peripheral zone (PZ) were carefully selected from the surgically excised prostatectomy and cystoprostatectomy specimens, which were snap-frozen within 30 min of removal from the patients and stored at −80 °C. Matching formalin-fixed paraffin-embedded (FFPE) samples were fixed in 10% neutral buffered formalin for 24 h at room temperature. Then, the tissues underwent serial dehydration with ethanol, followed by p-xylene cleaning, and paraffin infiltration, by a Leica ASP300S tissue processor (Leica Microsystems Inc., Buffalo Grove, IL). The FFPE sections were stained with hematoxylin and eosin (H&E) tissue stains using a Leica ST5010 Autostainer XL. All biospecimens were deidentified. The study pathologist (P. Murugan) reviewed the H&E stained FFPE sections for pathology markers associated with PC, such as atrophy, inflammation, and high-grade prostatic intraepithelial neoplasia (HGPIN) and quantified the samples as mild, moderate, or severe. Specimens selected for DNA adduct measurements were from largely tumor-free tissue. The inflammation, atrophy, and HGPIN biomarkers were evaluated later. Prognostic variables, including Gleason score and tumor stage, were recorded from the pathology database in patients diagnosed with PC, and the PSA assay was conducted by chemiluminescence using a Siemens Vista analyzer.70 (link)-72 (link)
Guo J., Koopmeiners J.S., Walmsley S.J., Villalta P.W., Yao L., Murugan P., Tejpaul R., Weight C.J, & Turesky R.J. (2022). The Cooked Meat Carcinogen 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine Hair Dosimeter, DNA Adductomics Discovery, and Associations with Prostate Cancer Pathology Biomarkers. Chemical research in toxicology, 35(5), 703-730.
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