To determine the effect of therapeutic vaccines on different T cell subsets in mice, blood (100 mL) was collected at the indicated time points in 12 mice/group. Total number of lymphocytes was determined. CD4+ and CD8+ T cell populations were determined on days 12 and 27 by staining with PE-labeled anti-mouse CD4 and FITC-labeled anti-mouse CD8 antibodies (eBioscience, San Diego, CA). Then CD4+ and CD8+ T cell populations were measured by flow cytometry. To determine IFN-r+ CD8+ and CD4+ Foxp3+ T cell populations, cells were stained with FITC-labeled anti-mouse CD8 or FITC-labeled anti-mouse CD4 (Clone RM4-5, concentration 0.5 mg/m, Biolegend), fixed, permeabilized, and stained with PE-labeled anti-IFN-γ or PE-labeled anti-FoxP3 antibody (Biolegend). T cell subsets were measured using flow cytometry. All experiments were carried out in triplicate.
Pe labeled anti foxp3 antibody
Manufactured by BioLegend
Sourced in United States
The PE-labeled anti-FoxP3 antibody is a flow cytometry reagent that specifically binds to the Forkhead box P3 (FoxP3) transcription factor, which is a key regulator of regulatory T cell (Treg) development and function. The PE (Phycoerythrin) fluorescent dye is conjugated to the antibody, allowing for the detection and quantification of FoxP3-expressing cells by flow cytometry.
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2 protocols using pe labeled anti foxp3 antibody
1
Therapeutic Vaccine Effects on T Cell Subsets
2
T-cell Subset Analysis of Immunotherapy
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To determine the therapeutic effect of the SA‐IL‐2‐modified vaccine together with PD‐1 blockade on T‐cell subsets, 100 μL blood was collected from each group on day 19 after tumor injection. To detect CD4+ and CD8+ T‐cell subsets, cells were stained with FITC‐labeled anti‐mCD4 and APC‐labeled anti‐mCD8 (eBioscience). To detect IFN‐γ+CD8+, PD‐1+CD8+ and CD4+Foxp3+ T‐cell subsets, cells were first stained with APC‐labeled anti‐mCD8 or FITC‐labeled anti‐mCD4 (Biolegend, San Diego, CA, USA), and then stained with PE‐labeled anti‐IFN‐γ, PE‐labeled anti‐PD‐1 or PE‐labeled anti‐FoxP3 antibody (Biolegend). T‐cell subsets were detected by flow cytometry.
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