Sixty SD rats were randomly divided into six groups: control, ORN, ORN + QJT-low, ORN + QJT-middle, ORN + QJT-high, and ORN + QJT + radicicol. The AZS model was constructed using ornidazole (ORN). Rats in the control and ORN groups were gavaged with 200 mg/kg/d ORN [dissolved in 1% sodium carboxymethylcellulose (CMC-Na, Solarbio, Beijing, China)] and the same amount of 1% CMC-Na for six consecutive weeks. Rats in ORN + QJT-low, ORN + QJT-middle and ORN + QJT-high were gavaged with 200 mg/kg/d ORN for three consecutive weeks and then gavaged with QJT [its preparation shown in our recent study (Li G, Zhang, et al. 2021 (link))] at a concentration of 0.17, 0.33, and 0.67 g/mL before ORN administration in weeks 4–6 respectively. Rats in ORN + QJT + radicicol were gavaged with 200 mg/kg/d ORN for three consecutive weeks and then gavaged with 0.67 g/mL QJT and synchronously injected intraperitoneally with 20 mg/kg radicicol (CAS NO. 12772-57-5, AdooQ BioScience, CA, USA) in weeks 4–6. After the last treatment for 12 h, rats were intraperitoneally anesthetized with 25% ethyl carbamate (4 mL/kg) to obtain testis tissues, which were removed and stored for subsequent assays.
Cmc na
Manufactured by Solarbio
Sourced in China
CMC-Na is a sodium carboxymethylcellulose product. It is a cellulose derivative with sodium carboxymethyl groups attached to some of the hydroxyl groups of the cellulose backbone. CMC-Na is a white to off-white powder with a slight characteristic odor.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Solarbio (2 mentions)
Rpmi media, by Thermo Fisher Scientific (1 mentions)
Puromycin, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Takara Bio (1 mentions)
Gallic acid, by Solarbio (1 mentions)
Radicicol, by Adooq (1 mentions)
Curcumin, by Merck Group (1 mentions)
Alginate, by Solarbio (1 mentions)
Cellobiose, by Solarbio (1 mentions)
Plasmid miniprep kit, by Transgene (1 mentions)
Isopropyl β d 1 thiogalactopyranoside iptg, by Merck Group (1 mentions)
Ampicillin, by Merck Group (1 mentions)
7 protocols using cmc na
1
Ornidazole-Induced Rat Testicular Injury
2
Isolation and Characterization of Latifolin
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Latifolin (chemical name (−)-(R)-latifolin, PubChem No. 340211) was provided by the Jiangxi University of Traditional Chinese Medicine (Broadwood Dalbergia was purchased from Fangchenggang City, Guangxi Province, China). Latifolin was isolated and prepared from the core material of lignum dalbergiae odoriferae. The preparation process entailed crushing lignum dalbergiae odoriferae heartwood and extracting with 70% ethanol by heated reflux extraction. The extract was then concentrated and extracted with water to make a suspension, followed by petroleum ether extraction. The extract was then analyzed by silica gel chromatography. The gradient elution was carried out with petroleum ether-ethyl acetate as the eluent to obtain the monomeric compound. The structure was determined by 1H-NMR, 13C-NMR (Fig. 1 A). The purity of the extract was determined to be >95%. Latifolin samples were dissolved in dimethylsulfoxide (DMSO) for in vitro experiments, and DMSO were obtained from Solarbio (Peking, China); the purity being >99.5%, and samples were evenly dispersed in 0.5% sodium carboxyl methyl cellulose (CMC-Na) as a suspension for in vivo experiments. CMC-Na were purchased from Yuanye Biological Technology Co, Ltd (Shanghai, China), and the degree of substitution (DS) is 0.7.
3
Synthesis and Characterization of N69B
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Chemical name of the compound N69B is (E)-1-(4-(4-Methylpiperazine-1-carbonyl) phenyl)-3-(4-((4-morpholino-6-styryl-1, 3, 5-triazin-2-yl)amino)phenyl)urea.The compound was synthesized at Bellen Chemistry Co, Ltd. and provided by Luoda Biosciences, Inc. The compound structure and purity analyses are shown in Supplementary Fig. 1, Supplemental digital content 1, http://links.lww.com/ACD/A327 . It has the formula of C34H37N9O3, the measured molecular weight of 619.8 (MS, [M+H]+) and the purity of 97.5% (HPLC).
The compound was either dissolved in DMSO (Solarbio, Beijing, China) or suspended in 0.5% CMC-Na (Solarbio, Beijing, China) for in-vitro and in-vivo studies. The stock solution of 20 mM was stored at −20°C and diluted with RPMI media (Gibco, USA) to working concentrations prior to use; the stock suspension of 100 mg/ml was stored at 4°C for a maximal duration of one week and warmed at room temperature prior to use.
The compound was either dissolved in DMSO (Solarbio, Beijing, China) or suspended in 0.5% CMC-Na (Solarbio, Beijing, China) for in-vitro and in-vivo studies. The stock solution of 20 mM was stored at −20°C and diluted with RPMI media (Gibco, USA) to working concentrations prior to use; the stock suspension of 100 mg/ml was stored at 4°C for a maximal duration of one week and warmed at room temperature prior to use.
4
Cytokine-Induced Cell Proliferation Protocol
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Handelin (HH251907) was obtained from Herbest Bio. (Baoji, China). TNF‐α (CF09) was purchased from novoprotein (Suzhou, China). LPS (L2630) was obtained from Sigma‐Aldrich Chemical Co. (St. Louis, MO, USA). Reagents used in the culture of cells including FBS and DMEM high glucose culture medium were purchased from VivaCell (Shanghai, China). TRIzol reagent (9109), Prime Script RT‐PCR and SYBR® Premix Ex Taq™ (Tli RNaseH Plus) kits (RR047A) were attained from TaKaRa Bio (Beijing, China). Puromycin (A1113803) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). CMC‐Na (C8621) was obtained from Solarbio life science (Beijing, China). MTS Cell Proliferation and Cytotoxicity Detection Kit (G3580) was purchased from Promega (Beijing, China). All other reagents were supplied by Bmassay Bio (Nanchang, China).
5
Extraction and Identification of QIGs
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QIGs were purchased from Guangzhou and identified at the Xinjiang Medical University with voucher number 20190809. Poloxamer 407 (P407; Solarbio, China), poloxamer 188 (P188; Solarbio, China), CMC-Na (Solarbio, China), gallic acid (assigned purity >98%, Solarbio, China), HPMC (Macklin, China), methanol (GR, Sigma-Aldrich, USA), phosphoric acid (AR, Tianjin Zhiyuan Chemical Reagent, China), phosphate-buffered saline (PBS) powder (pH 7.3; ORIGENE, China), and new indocyanine green (IR820) (Macklin, China) were used in experiments.
6
Curcumin Bioavailability Enhancement via CMC-Na
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Curcumin (C7727, total purity >94.0%) was purchased from Sigma-Aldrich Trading Co (Shanghai, China). Sodium carboxymethyl cellulose (CMC-Na) (C8621, viscosity 800–1200) was purchased from Beijing Solarbio Science and Technology Co. (Beijing, China). As Curcumin is insoluble in water, we chose CMC-Na (a food additive usually used as a viscosity modifier) to improve its solubility and increase its bioavailability. Curcumin powder was poured into 0.5% CMC-Na solution and dissolved under magnetic stirring for 10 min. Curcumin (100 mg/kg/day) was administered to the mice intragastrically twice daily (once in the morning and the evening) for 14 days after the 14th postoperative day.
7
Recombinant Protein Expression Protocol
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Mono-galacturonic acid, di-galacturonic acid, tri-galacturonic acid, polygalacturonic acid, citrus pectin, apple pectin, rhamnogalacturonic acid, CMC-Na, Avicel, beechwood xylan, alginate, and cellobiose were purchased from Solarbio Science & Technology (Beijing, China). The vector pEAZY blunt-E2, competent cells E. coli BL21 (DE3) pLysS, and Plasmid mini Prep Kit were purchased from TransGen Biotech (Beijing, China). Isopropyl β-D-1-thiogalactopyranoside (IPTG) and ampicillin were purchased from Sigma-Aldrich (St.
Louis, MO, USA).
Louis, MO, USA).
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