Kh2po4

The active substance used was cetirizine dihydrochloride (Jubilant Lifesciences Ltd, India; CTZ). The excipients used for the study SeDeM were talc (Fagron, Spain), magnesium stearate (Fagron, Spain), and colloidal silicon dioxide (Aerosil ®, Fagron, Spain). The excipients used in the factorial design were mannitol (Fagron, Spain) and polyvinylpyrrolidone Ph. Eur (Fagron, Spain). The reagents used for the preparation of phosphate buffers solutions (solubility studies) were: 37% HCl (Panreac, Barcelona), NaCl (Panreac, Barcelona), NaOH (Panreac, Barcelona) and KH₂PO₄ (Panreac, BCN).

Sodium alginate was purchased from Fagron Iberica (Terrassa, Spain). Ketorolac tromethamine and Nipagin were obtained from Sigma-Aldrich (Barcelona, Spain). Nipasol was acquired from Acofarma (Barcelona, Spain), and Na₂HPO₄ and KH₂PO₄ were supplied by Panreac (Barcelona, Spain). PBS pH 7.6, gentamicin sulphate, and bovine serum albumin were provided by Sigma (St. Louis, MO, USA); methanol was obtained from Merck, Darmstadt, Germany. The adhesive tapes were obtained from D-squame, (Cuderm Co., Dallas, TX, USA).
The purified water was obtained from a Milli-Q1 Gradinet A10 system apparatus (Millipore Iberica S.A.U., Madrid, Spain). All the other chemicals and reagents used in the study were of analytical grade.

Sodium alginate was purchased from Fagron Iberica (Terrassa, Spain). Ketorolac tromethamine and Nipagin were obtained from Sigma-Aldrich (Barcelona, Spain). Nipasol was acquired from Acofarma (Barcelona, Spain), Hyaluronic acids were obtained from Fagron Iberica (Terrassa, Spain); Na2HPO4 and KH2PO4 were supplied by Panreac (Barcelona, Spain), NaCl and KCl were from Merck (Darmstadt, Germany), CaCl from Ferosa (Spain) Hepes was obtained from Fagron Iberica (Terrassa, Spain) and glucose from Sigma-Aldrich (Barcelona, Spain). The Millipore Express^® PLUS 0.45 µm PES Membrane was from Merck (Darmstadt, Germany), the Nylon membrane Filter with 0.45 µm pore size from Teknokroma (Barcelona, Spain) and the biomimetic membrane PermeaPad^® from InnoME GmbH (Espelkamp, Germany).
The purified water was obtained from a Milli-Q1 Gradient A10 system apparatus (Millipore Iberica S.A.U., Madrid, Spain). All the other chemicals and reagents used in the study were of analytical grade.

C. glabrata strains were tested regarding their capacity to form biofilm on a polystyrene surface, recurring to the Presto Blue Cell Viability Assay. For that, the C. glabrata strains were grown in SDB (pH 5.6) medium and collected by centrifugation at mid-exponential phase. The cells were inoculated with an initial OD_600nm = 0.05 ± 0.005 in 96-well polystyrene microtiter plates (Greiner) in SDB (pH 5.6) medium. Cells were cultivated at 30 °C during 24 ± 0.5 h with mild orbital shaking (70 r.p.m.). After the incubation time, each well was washed two times with 100 µL of sterile phosphate-buffered saline (PBS) pH 7.4 (PBS contained per liter: 8 g NaCl (Panreac), 0.2 g KCl (Panreac), 1.81 g NaH₂PO₄.H₂O (Merck), and 0.24 g KH₂PO₄ (Panreac), to remove the cells unattached to the formed biofilm. Then, Presto Blue reagent was prepared in a 1 : 10 solution in the medium used for biofilm formation, adding 100 µL of the solution to each well. Plates were incubated at 37 °C for 30 min. Afterwards, absorbance reading, at the wavelength of 570 and 600 nm for reference, was determined in a microplate reader (SPECTROstar Nano, BMG Labtech).

Carbamazepine (CBZ), BPA, estrone (E1), 17b-estradiol (E2), 17a-ethinylestradiol (EE2), MnSO₄, C₂H₃NaO₂, H₂O₂, 2,6-dimethoxyphenol (DMP), and Tween-80 were purchased from Sigma-Aldrich (Barcelona, Spain). Glucose, KH₂PO₄, NaC₂H₃O₂, and CaCl₂ were purchased from Panreac (Barcelona, Spain), meat peptone from Cultimed (Barcelona, Spain), yeast extract from iNtRON Biotechnology (Seongnam, South Korea), and MgSO₄·7H₂O from Fluka (Steinheim, Germany). Wheat straw was obtained from a local supplier (Carral, A Coruña, Spain) and stored at room temperature until use. The contents of cellulose and hemicellulose, as well as fermentable sugars in the wheat straw, are presented in Supplemental Table S1. These parameters are decisive in identifying the concentration of the C source present in the wheat straw for the formulation of the fermentation medium.

Sodium alginate was purchased from Fagron Iberica (Barcelona, Spain). Calcium chloride (CaCl₂), ketorolac tromethamine (KT), and nipagin were purchased from Sigma-Aldrich (Barcelona, Spain). Nipasol from Acofarma (Barcelona, Spain) and Na₂HPO₄, KH₂PO₄, and NaCl by Panreac (Barcelona, Spain). Methanol from Merck (Darmstadt, Germany). The purified water was obtained from a Milli-Q1 Gradient A10 system apparatus (Millipore Iberica S.A.U. Madrid, Spain). All the other chemicals and reagents used in the study were of analytical grade.

Yeast cultures were diluted to OD_600nm of 0.15 using phosphate-buffered saline (PBS, 137 mM NaCl (Thermo Fisher Scientific, Waltham, MA, USA), 2.7 mM KCl (Merck, Darmstadt, Germany), 10 mM Na₂HPO₄ (Panreac, Barcelona, Spain), 1.8 mM KH₂PO₄ (Panreac, Barcelona, Spain), pH 7.4), and 13.3 μL of culture was spotted onto a SC glucose plate. Upon 72 h of incubation at 26 °C, colonies were covered with a nitrocellulose membrane (GE Healthcare, Chicago, IL, USA). After 24 h at 26 °C, membranes were removed and washed with TBS-0.05% (v/v) Tween (Sigma Aldrich, St. Louis, MO, USA) solution to clear away all cells that remained attached. Detection of CPY secretion was carried out by immunoblotting, with mouse anti-CPY primary antibody (1:2000, Invitrogen, Waltham, MA, USA) and anti-mouse IgG-peroxidase secondary antibody (1:5000, Molecular Probes, Eugene, OR, USA).