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Apc conjugated anti foxp3 fjk 16s

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The APC-conjugated anti-Foxp3 (FJK-16s) is a fluorescently labeled antibody that binds to the Foxp3 transcription factor. Foxp3 is a marker for regulatory T cells (Tregs). The APC (Allophycocyanin) fluorescent label allows for the detection of Foxp3-positive cells using flow cytometry.

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Lab products found in correlation

Foxp3 staining buffer set, by Thermo Fisher Scientific (2 mentions) Facsverse, by BD (2 mentions) F4 80 bm8, by Thermo Fisher Scientific (2 mentions) Foxp3 fixation permeabilization concentrate and diluent, by Thermo Fisher Scientific (2 mentions) Pe cy7 conjugated anti fr4, by Thermo Fisher Scientific (2 mentions) Pacific blue conjugated t bet, by BioLegend (2 mentions) Macsquantify software, by Miltenyi Biotec (1 mentions) Anti mouse rat foxp3 staining kit, by Thermo Fisher Scientific (1 mentions) Pe cy7 conjugated anti cd3 145 2c11, by BioLegend (1 mentions)

Close spelling variants of this product

FJK-16s (166 citations) Anti-Foxp3 (119 citations) Foxp3 (FJK-16s, (98 citations) Foxp3-APC (77 citations) Anti-Foxp3-APC (68 citations) Anti-Foxp3 (FJK-16s) (63 citations) FoxP3 APC (FJK-16s, (16 citations) APC anti-FoxP3 (FJK 16s, (6 citations) APC-conjugated anti-Foxp3 (6 citations)

5 protocols using apc conjugated anti foxp3 fjk 16s

1

Multiparametric Immune Cell Profiling

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PE- and eFluor450-conjugated anti-CD4 (GK1.5), PerCP/Cy5.5- and APC/eFlour780-conjugated anti-CD8 (53-6.7), PE-conjugated anti-CD25 (PC61.5), PE-conjugated anti-Ki67 (SolA15), PE-conjugated anti-CTLA-4 (UC10-4B9), PE-conjugated anti-Tim-3 (RMT3-23), PE/Cyanine7- and APC/eFlour780-conjugated anti-CD44 (MI7), FITC-conjugated anti-CD62L (MEL-14), PE/Cyanine5-conjugated anti-ICOS (7E.17G9), PerCP/Cyanine5.5-conjugated anti-CD45.1 (A20), PE/Cyanine7-conjugated anti-GITR (DTA-1), FITC-conjugated anti-LAG-3 (eBioC9B7W), PE-conjugated anti-PD-1 (RMP1-30), and APC-conjugated anti-Foxp3 (FJK-16s) were obtained from eBioscience (San Diego, CA). FITC-conjugated anti-H-2Kd (SF1-1.1) and FITC-conjugated anti-Stat5 (pY694) were obtained from BD Bioscience. PE/Cyanine7-conjugated anti-CCR4 (2G12) and PE/Cyanine7-conjugated anti-CCR7 (4G12) was obtained from Biolegend. Intracellular FoxP3, Ki-67, and Stat5 were stained with an anti-mouse/rat Foxp3 staining kit (eBioscience, San Diego, CA). Cells were stained in phosphate-buffered saline containing 2% fetal calf serum, and sorted on a MACSQuant system with MACSQuantify software (Miltenyi Biotec, Bergisch Gladbach, Germany). Data were analyzed in FlowJo (Treestar, Ashland, OR)
Meguri Y., Asano T., Yoshioka T., Iwamoto M., Ikegawa S., Sugiura H., Kishi Y., Nakamura M., Sando Y., Kondo T., Sumii Y., Maeda Y, & Matsuoka K.I. (2022). Responses of regulatory and effector T-cells to low-dose interleukin-2 differ depending on the immune environment after allogeneic stem cell transplantation. Frontiers in Immunology, 13, 891925.
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2

Characterization of Regulatory T Cells

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Cells were collected at scheduled time points and stained with PerCP-Cy5.5-conjugated anti-CD3 (145-2C11; eBiosciences, San Diego, CA). Intracellular staining was performed utilizing the Foxp3 staining buffer set (eBioscience, San Diego, CA) and stained with APC-conjugated anti-Foxp3 (FJK-16s; eBiosciences, San Diego, CA). The purity of pDCs was confirmed by staining: Ab of PerCP-eFluor 710 CCR9 (eBioCW-1.2; eBiosciences, San Diego, CA), Ab of FITC-conjugated B220 (RM4-5; BioLegend, San Diego, CA), Ab of PE-conjugated CD317 (PDCA-1, BST2) (eBio129c eBiosciences, San Diego, CA). For negative controls, unstained cells or cells stained with each isotype-controlled mAb were utilized. All samples were analyzed on Accuri C6 FTM (BD Biosciences) or FACSverse (BD Biosciences) with FlowJo software (Tree Star). Figures from flow cytometry are representative of the triplicate samples.
Oh N.A., O’Shea T., Ndishabandi D.K., Yuan Q., Hong S., Gans J., Ge J., Gibney S., Chase C., Yang C., Rosales I., Shinoda K., Drew B., Kojima L., Russell P.S., Madsen J.C., Colvin R.B, & Alessandrini A. (2020). Plasmacytoid dendritic cell driven induction of Tregs is strain-specific and correlates with spontaneous acceptance of kidney allografts. Transplantation, 104(1), 39-53.
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3

Comprehensive T Cell Immunophenotyping

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T cells were interrogated with allophycocyanin (APC)–conjugated anti-CD25 (PC61.5), eFluor 450-conjugated anti-CD73 (eBioTY/11.8), PE-Cy7–conjugated anti-FR4 (eBio12A5), Alexa Fluor 700–conjugated anti-CD44 (IM7), PerCP-Cy5.5-conjugated anti-CD45.1 (A20), V500–conjugated anti-CD4 (RM4-5) (eBiosciences). T cells suspensions were also stained with APC–eFluor780–conjugated anti-B220 (RA3-6B2), -CD11b (MI-70), -CD11c (N418) (all eBiosciences), and -F4/80 (BM8; Invitrogen, Carlsbad, CA), for use as dump channel reagents. Stained T cells were then treated with Foxp3 Fixation/Permeabilization Concentrate and Diluent and stained with APC–conjugated anti-Foxp3 (FJK-16s) (eBioscience). In some experiments cells were also stained with Pacific Blue–conjugated T-bet (BioLegend, San Diego, CA) following fixation/permeabilization.
Kalekar L.A., Schmiel S.E., Nandiwada S.L., Lam W.Y., Barsness L.O., Zhang N., Stritesky G.L., Malhotra D., Pauken K.E., Linehan J.L., O’Sullivan M.G., Fife B.T., Hogquist K.A., Jenkins M.K, & Mueller D.L. (2016). CD4+ T cell anergy prevents autoimmunity and generates regulatory T cell precursors. Nature immunology, 17(3), 304-314.
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4

Flow Cytometric Characterization of Lymphocyte Subsets

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Cells were stained with PE-conjugated anti-H-2Dd mAb (34-5-8S; BD Pharmingen) to distinguish donor cells from recipient cells in combination with four different types of fluorescent staining for the following specific lineage markers: PerCP-Cy5.5-conjugated anti-CD8 (53-6.7), APC-conjugated anti-CD19 (MB19-1) (eBioscience); anti-CD49b (Dx5), APC-Cy7-cinjugated anti-CD4 (RM4-5) or PE-Cy7-conjugated anti-CD3 (145-2C11) (BioLegend, San Diego, CA). Intracellular staining was performed by permeabilization of cell membranes (Foxp3-staining buffer set; eBioscience), followed by staining with APC-conjugated anti-Foxp3 (FJK-16s; eBioscience). Isotype-matched immunoglobulin staining served as negative controls. All samples were analyzed on FACSVerse™ (BD Biosciences) with FlowJo software (Tree Star Data Analysis Software, Ashland, OR).
Shinoda K., Akiyoshi T., Chase C.M., Farkash E.A., Ndishabandi D.K., Raczek C.M., Sebastian D.P., Pelle P.D., Russell P.S., Madsen J.C., Colvin R.B, & Alessandrini A. (2014). Depletion of Foxp3+ T Cells Abrogates Tolerance of Skin and Heart Allografts in Murine Mixed Chimeras Without the Loss of Mixed Chimerism. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 14(10), 2263-2274.
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5

Comprehensive T Cell Immunophenotyping

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T cells were interrogated with allophycocyanin (APC)–conjugated anti-CD25 (PC61.5), eFluor 450-conjugated anti-CD73 (eBioTY/11.8), PE-Cy7–conjugated anti-FR4 (eBio12A5), Alexa Fluor 700–conjugated anti-CD44 (IM7), PerCP-Cy5.5-conjugated anti-CD45.1 (A20), V500–conjugated anti-CD4 (RM4-5) (eBiosciences). T cells suspensions were also stained with APC–eFluor780–conjugated anti-B220 (RA3-6B2), -CD11b (MI-70), -CD11c (N418) (all eBiosciences), and -F4/80 (BM8; Invitrogen, Carlsbad, CA), for use as dump channel reagents. Stained T cells were then treated with Foxp3 Fixation/Permeabilization Concentrate and Diluent and stained with APC–conjugated anti-Foxp3 (FJK-16s) (eBioscience). In some experiments cells were also stained with Pacific Blue–conjugated T-bet (BioLegend, San Diego, CA) following fixation/permeabilization.
Kalekar L.A., Schmiel S.E., Nandiwada S.L., Lam W.Y., Barsness L.O., Zhang N., Stritesky G.L., Malhotra D., Pauken K.E., Linehan J.L., O’Sullivan M.G., Fife B.T., Hogquist K.A., Jenkins M.K, & Mueller D.L. (2016). CD4+ T cell anergy prevents autoimmunity and generates regulatory T cell precursors. Nature immunology, 17(3), 304-314.
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