Anti lamin b

The cells were immersed in PBS and seeded in glass slides at a concentration of 1 × 10⁴/ml. The slides were fixed in 4% paraformaldehyde for 15 minutes, then immersed in PBS 3 times for 3 minutes each time, and subsequently in 0.5% Triton X‐100 in PBS at room temperature for 20 minutes. Next, the cells were blocked with 5% skim milk powder in PBST for 30 minutes at room temperature, and the following diluted primary antibodies were added: anti‐Lamin B, BOSTER, China; anti‐β‐actin, Abcam, USA; anti‐Lamin A, Abcam, USA. The antibodies used were rabbit anti‐human and diluted at a concentration of 1:200. The cells were incubated overnight at 4°C and subsequently with the fluorescent secondary antibody (1:100, mouse anti‐rabbit antibody, Abcam, USA) for 1 hour at room temperature. Finally, the slides were washed in PBST 3 times for 3 minutes each and observed under a fluorescence microscope.

A NE-PER nuclear and cytoplasmic extraction kit (Thermo Scientific, Rockford, IL) was used to extract nuclear and cytoplasmic proteins according to the manufacturer’s protocol. Each 20 μg protein sample was separated on an 8/10% sodium dodecyl sulfate-polyacrylamide gel. The proteins were then transferred onto polyvinylidene difluoride membranes (EMD Millipore, Darmstadt, Germany) using the Trans-Blot Turbo™ blotting system (Bio-Rad). After transferring, membranes were blocked with 5% BSA at room temperature for 2–3 h. The membranes were then incubated overnight at 4°C with anti-SREBP-2 (1:600; Abcam, Cambridge, UK), anti-HMGCR (1:5,000; Abcam), anti-LDLR (1:500; Abcam), anti-proprotein convertase subtilisin/kexin type 9 (PCSK9) (1:600; Proteintech, Wuhan, China), anti- CYP-7α (1:1,000; Santa Cruz Biotechnology, Santa Cruz, CA), anti-HBsAg (1:1,000; Abcam), anti-HBV xAg (1:200; Santa Cruz Biotechnology), anti-Hep B cAg (1:200; Santa Cruz Biotechnology), anti-GAPDH (1:10,000; Abcam), or anti-lamin B (1:300; Boster, Wuhan, China) primary antibodies, respectively. After washing with TBST, the membranes were incubated with horseradish peroxidase-linked secondary antibodies (1:5,000; Boster). An enhanced chemiluminescence Western blotting substrate kit (BioVision, SanFrancisco, CA) was used to detect specific proteins.