Ff01 731 137 25

Manufactured by IDEX Corporation

The FF01-731/137-25 is a laboratory equipment product manufactured by IDEX Corporation. It serves as a high-performance optical filter, designed to precisely select specific wavelengths of light for various scientific and research applications.

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Lab products found in correlation

Orca flash4.0 v3, by Hamamatsu Photonics (2 mentions) Xlpln25xwmp2, by Olympus (1 mentions) Ff01 609 54 25, by IDEX Corporation (1 mentions) Ff01 525 45 25, by IDEX Corporation (1 mentions)

3 protocols using ff01 731 137 25

Multimodal Imaging and Patch Clamp Microscopy

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These experiments were performed using a home-built multimodal microscope with a patch clamp add on, the design of which has been described recently (39 ). Epifluorescence imaging was performed using two laser beams at 488 nm (OBIS 488 LX, Coherent) and 532 nm (MLL-III-532, CNI) focused onto the back aperture of a 25X objective (XLPLN25XWMP2, Olympus). Illumination power of 11.2 mW/mm² and 37.23 mW/mm² were used for brightness screening for 488 nm and 532 nm, respectively. For patch clamp characterization, the 532 nm intensity was 87.6 mW/mm². The emission light was filtered using a multiband dichroic mirror (Di03-R405/488/532/635-t3-32x44, Semrock) and a 552 to 779.5 bandpass filter (FF01-731/137-25, Semrock). The images were acquired at a frame rate of 100 or 500 Hz using an sCMOS camera (ORCA Flash4.0 V3, Hamamatsu; 2048 x 2048 pixels, 6.5 μm pixel size). The voltage pulses, illumination, and camera recording were synchronized using the National Instruments DAQ (USB-6363). All software for controlling the hardware, image acquisition and analysis were custom written in Python (39 ).

Ganapathy S., Meng X., Mossel D., Jagt M, & Brinks D. (2023). Expanding the family of genetically encoded voltage indicators with a candidate Heliorhodopsin exhibiting near-infrared fluorescence. The Journal of Biological Chemistry, 299(6), 104771.

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dSTORM Imaging on Customized Microscopy Setup

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dSTORM was performed on a custom setup built around an Olympus IX-73 microscope stand (IX-73; Olympus America, Waltham, MA) using a 100×/1.35 NA silicone oil-immersion objective lens (FV-U2B714; Olympus America) and a PIFOC objective positioner (ND72Z2LAQ; Physik Instrumente, Karlsruhe, Germany). Sample excitation and activation were achieved with a 642-nm laser (2RU-VFL-P-2000-642-B1R; MPB Communications, Pointe Claire, QC, Canada) and a 405-nm laser (DL-405-100; Crystalaser, Reno, NV), respectively. Both lasers were focused to the back aperture of the objective lens and offset from the optical axis so that the sample was illuminated in HILO mode (Tokunaga et al., 2008 (link)). The filter turret contained a quad band dichroic mirror (Di03-R405/488/561/635-t1; Semrock, Rochester, NY) for illumination with both lasers. Relay lenses arranged in a 4f alignment resulted in a final magnification of 52.7 at the sCMOS camera (Orca-Flash4.0v3, Hamamatsu, Tokyo, Japan) with an effective pixel size of 120 nm. The fluorescence signal passed through a bandpass filter (FF01-731/137-25; Semrock) placed just before the camera. Localization of single molecules was performed as described previously (Huang et al., 2013 (link)).

Ren Y., He Y., Brown S., Zbornik E., Mlodzianoski M.J., Ma D., Huang F., Mattoo S, & Suter D.M. (2019). A single tyrosine phosphorylation site in cortactin is important for filopodia formation in neuronal growth cones. Molecular Biology of the Cell, 30(15), 1817-1833.

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Multi-channel Fluorescence Microscopy

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The multi-band optics allows for detection of four open emission windows (420 – 480; 500 – 550; 565 – 630; and 650 – 800 nm, Figure 1), and three simultaneously without moving parts (see Figure 2), which greatly enhances drift stability of the microscope. A second MIM-CUBE-II-K cube on the top port splits the ‘blue’ emission path: it holds a 2-mm-thick emission dichroic (T490pxrxt-A/R coated, Chroma), and a bandpass emission filter (FF01-525/45-25, Semrock). A third MIM-CUBE-II-K cube on the top port holds a 2 mm thick emission dichroic (T560lpxr – lot 253099, Chroma), and splits the ‘orange’ emission channel, which is filtered further by a band-pass emission filter (FF01-609/54-25, Semrock). A bandpass filter (FF01-731/137-25, Semrock) filters the transmitted emitted light of the third, ‘far red’ emission channel. Three images are formed using the Olympus objective and three custom tube lenses that are placed in the infinity space of each of the three channels (LAO-300.0, Melles Griot), resulting in 100x overall magnification.

English B.P, & Singer R.H. (2015). A three-camera imaging microscope for high-speed single-molecule tracking and super-resolution imaging in living cells. Proceedings of SPIE--the International Society for Optical Engineering, 9550, 955008-.

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