β tubulin antibody sc 23949

Western blot analysis was performed using standard techniques^{8 (link)}.
The primary antibodies were: HELLS (Rabbit mAb#7998, 1:1000 Cell Signaling Technology), γ PAK2 (E-9 Mouse mAb sc-373740, 1:1000 Santa Cruz Biotechnology, Inc), RHOA (26C4 Mouse mAb sc-418, 1:1000 Santa Cruz Biotechnology, Inc), RHOU (Rabbit, PA5-69128, 1:500 Invitrogen), YY1 (Rabbit, D3D4Q, 1:1000, Cell Signaling Technology), β-tubulin antibody (sc-23949, 1:100, Santa Cruz Biotechnology, Inc) and GAPDH (Rabbit mAb#2118, 1:2000, Cell Signaling Technology)
All secondary antibodies (rabbit and mouse) were HRP-conjugated (GE Healthcare) and diluted 1:3000.
Densitometric analysis was performed using the ImageJ software.

Cells were spotted on glass slides using Cytospin (Thermo Scientific), fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.1% Triton X-100 for 3 min. Dots were blocked in 1% PBS-BSA solution for 40 min at room temperature and incubated with phalloidin (Alexa Fluor^® 488, Thermo Fisher) for actin staining for 50 min. Dots were washed in PBS for three times and nuclei were stained with DAPI. For microtubules staining we used β-tubulin antibody (sc-23949, 1:100, Santa Cruz Biotechnology, Inc). Immunofluorescences were detected with Nikon Eclipse (Ni) microscope using 60X.