The largest database of trusted experimental protocols

Ab5201

Manufactured by Abcam

Ab5201 is a laboratory equipment product. It is designed for use in scientific research and experiments. The core function of Ab5201 is to [DESCRIPTION_NOT_AVAILABLE].

Automatically generated - may contain errors

2 protocols using ab5201

1

Quantitative Western Blot Analysis of Neuronal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell or nuclear extracts from primary neurons or brain tissue were resolved on 3–8% Tris-acetate gels (Thermo Fisher, EA0375BOX) or 10% Tris-glycine gels and transferred to nitrocellulose membranes. Membranes were incubated overnight in the following primary antibodies: EP400 (Bethyl Labs, A300-541-A; Abcam, Ab5201; Abcam, 70301; 1:1,000); DMAP1 (Cell Signaling Technology, 13326; Santa Cruz, sc-373949; 1:500 or 1:1,000); TRRAP (Bethyl, A301-132A; 1:500 or 1:1,000); ARNT2 (in house; 1:1,000)28 (link); NPAS4 (in house, 1:1,000)14 (link); FOS (in house; 1:1,000); β-tubulin3 (Covance, MMS-435P; 1:5,000); GAPDH (Sigma-Aldrich, G9545; 1:5,000); histone H3 (Abcam, 1791; 1:10,000); NPAS3 (gift from S. McKnight; 1:1,000)55 (link); and HA (Cell Signaling Technology, C29F4; 1:1,000). Following washing, membranes were incubated with secondary antibodies conjugated to IRdye 700 or 800 and imaged using a LiCOR Odyssey instrument.
+ Open protocol
+ Expand
2

Comprehensive Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against p15 (C20, Santa-Cruz), p16 (EPR1473, Epitomics), p21 (C19, Santa-Cruz), GAPDH (MAB 374, Chemicon International), PanAcH4 (06-866, Millipore), H3K4me3 (pAb-003, Diagenode), HMGA1 (ab4078, Abcam), H2A.Z (ab4174, Abcam), p400 (ab5201, Abcam), RNA PolII S2P (ab5095, Abcam), SYDE2 (HPA027138, Sigma), DDAH1 (ab82908, Abcam) and CYR61 (sc-374129, Santa-Cruz) were purchased.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!