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Rifampicin

Manufactured by Meilun
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Sourced in China

Rifampicin is a chemical compound used as a key ingredient in laboratory equipment and instrumentation. It is a broad-spectrum antibiotic that inhibits bacterial RNA synthesis, making it a vital component in various analytical and research applications.

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Lab products found in correlation

Gentamicin, by Meilun (3 mentions) Ciprofloxacin, by Meilun (2 mentions) Tigecycline, by Meilun (2 mentions) Mueller hinton agar (mha), by Thermo Fisher Scientific (2 mentions) Erythromycin, by Meilun (2 mentions) Cefepime, by Meilun (1 mentions) Ceftazidime, by Meilun (1 mentions) Polymyxin b, by Meilun (1 mentions) Meropenem, by Meilun (1 mentions) Ampicillin, by Meilun (1 mentions) Amikacin, by Meilun (1 mentions) Minocycline, by Meilun (1 mentions) Chloramphenicol, by Meilun (1 mentions) Doxycycline, by Meilun (1 mentions) Levofloxacin, by Meilun (1 mentions) Imipenem, by Meilun (1 mentions) Azithromycin, by Meilun (1 mentions) Rifampicin, by Thermo Fisher Scientific (1 mentions) Dig high prime dna labeling and detection starter kit 2, by Roche (1 mentions) Clindamycin, by Meilun (1 mentions)

5 protocols using rifampicin

1

Characterization of Plasmids Carrying NDM-9 and MCR-1

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The number and size of the plasmid of the strains were characterized by S1-PFGE. The location of blaNDM-9 and mcr-1 genes was confirmed by Southern blotting and hybridization with a digoxigenin-labeled blaNDM-9 and mcr-1 probe using DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche Diagnostics). Conjugation transfer experiments were conducted to explore the transferability of plasmids with rifampicin-resistant E. coli 600 as the recipient strain as recipients, as described previously.17 (link) After that, using Mueller-Hinton agar (OXOID, Hampshire, United Kingdom) plates that contained both 200 mg/L rifampicin (Meilunbio, Dalian, China) and 2 mg/L meropenem to select blaNDM-9 carrying transconjugants, and 200 mg/L rifampicin with 2 mg/L colistin to select mcr-1 carrying transconjugants, respectively. The final identification of transconjugants, including MALDI-TOF/MS identification, resistance genes detection, and AST, to confirm whether the experiments succeed.
Liang G., Rao Y., Wang S., Chi X., Xu H, & Shen Y. (2021). Co-Occurrence of NDM-9 and MCR-1 in a Human Gut Colonized Escherichia coli ST1011. Infection and Drug Resistance, 14, 3011-3017.
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2

Antibacterial Agents: Comprehensive Evaluation

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A wide range of clinically-used antibacterial agents including ampicillin, ampicillin-sulbactam (2:1), cefepime, ceftazidime, doxycycline, minocycline, amikacin, gentamicin, ciprofloxacin, levofloxacin, chloramphenicol, imipenem, meropenem, tigecycline, polymyxin B, azithromycin and rifampicin were purchased from Meilun Biological (Dalian, Liaoning, China). Zinc lactate, stannous fluoride and furanone were obtained, respectively, from Yuanye Bio-Technology (Shanghai, China), Macklin Biochemical (Shanghai), and Sigma-Aldrich (St. Louis, Missouri, USA). Bacterial culture media, Muller-Hinton broth (MHB), cation-adjusted MH broth (CAMHB), and tryptic soy broth (TSB) medium were purchased from Haibo Biotechnology (Qingdao, Shandong, China).
Peng Q., Lin F, & Ling B. (2020). In vitro activity of biofilm inhibitors in combination with antibacterial drugs against extensively drug-resistant Acinetobacter baumannii. Scientific Reports, 10, 18097.
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3

Plasmid Characterization of NDM-producing M. morganii

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The number and size of plasmid of M. morganii L241 were determined with the S1 nuclease pulsed field gel electrophoresis (S1-PFGE) method, as described previously (Zheng et al., 2015 (link)). Southern blotting and hybridization using DIG-labeled blaNDM-specific probe were performed to estimate the location of blaNDM gene, while the transferability of NDM-carrying plasmid from the isolate was determined through the use of conjugation experiments, with rifampicin-resistant E. coli C600 as the recipient strain. Further to this, transconjugants were selected on Mueller-Hinton agar (OXOID, Hampshire, United Kingdom) plates that contained both 200 mg/L rifampicin (Meilunbio, Dalian, China) and 2 mg/L meropenem. Finally, a combination of MALDI-TOF/MS identification, blaNDM gene detection and antimicrobial susceptibility testing of the transconjugants were performed in order to confirm whether the plasmid was successfully transferred to the recipient.
Guo X., Rao Y., Guo L., Xu H., Lv T., Yu X., Chen Y., Liu N., Han H, & Zheng B. (2019). Detection and Genomic Characterization of a Morganella morganii Isolate From China That Produces NDM-5. Frontiers in Microbiology, 10, 1156.
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4

Synthesis and Characterization of ZG Compounds and Antibiotic Purchases

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ZG180, (R)- and (S)-ZG197 were synthesized in lab and fully characterized. ONC212 and ADEP 4 were purchased from Topscience and ChemPartner (Shanghai, China), respectively. Antibiotics of vancomycin, spectinomycin, erythromycin, ciprofloxacin, rifampicin, tetracycline, norfloxacin, clindamycin, and gentamicin, were purchased from Dalian Meilun Biotech. Oxacillin was purchased from Sigma-Aldrich. Anhydrous tetracycline (ATC) was purchased from APExBIO.
Wei B., Zhang T., Wang P., Pan Y., Li J., Chen W., Zhang M., Ji Q., Wu W., Lan L., Gan J, & Yang C.G. (2022). Anti-infective therapy using species-specific activators of Staphylococcus aureus ClpP. Nature Communications, 13, 6909.
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5

Antimicrobial Agents Evaluation Protocol

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The antimicrobials utilized in this work were cefazolin, gentamicin, erythromycin, tigecycline, rifampicin, daptomycin (purchased from Dalian Meilun Biotech, Dalin, China), ciprofloxacin, clindamycin, vancomycin (from the National Institutes for Food and Drug Control, Beijing, China), linezolid (Selleck Chemicals, Houston, TX, United States), and trimethoprim–sulfamethoxazole (Sigma–Aldrich, St Louis, MO, United States).
Yang H., Xu S., Huang K., Xu X., Hu F., He C., Shu W., Wang Z., Gong F., Zhang C, & Liu Q. (2020). Anti-staphylococcus Antibiotics Interfere With the Transcription of Leucocidin ED Gene in Staphylococcus aureus Strain Newman. Frontiers in Microbiology, 11, 265.
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