M. primoryensis were streaked onto a marine broth plate without antibiotic. A single colony of bacteria was used to inoculate marine broth (10 ml) and cultured at 4°C without shaking for 4 to 5 days until reaching an optical density (OD600nm) of 0.2 to 0.5 in a ThermoFisher Scientific Multiskan Go spectrometer (21 (link)). Both F. cylindrus and C. neogracile were grown as previously described (48 (link), 49 (link)). Thus, diatoms were grown in F/2 medium at 4°C with light and shaking. Bacteria cultured to an OD600nm of 0.2 to 0.4 were incubated with diatoms overnight at 4°C with light and shaking before being visualized with an Andor Zyla 4.2 Plus camera paired with an Olympus IX83 inverted fluorescence microscope modified with a custom-built cooling stage (50 (link)).
Multiskan go spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Multiskan Go spectrometer is a versatile UV-Vis microplate reader designed for a wide range of absorbance-based applications in life science research and diagnostics. The instrument features a monochromator-based optical system that provides accurate and reliable absorbance measurements across a wavelength range of 200 to 1000 nm.
Automatically generated - may contain errors
Lab products found in correlation
Celltiter 96 aqueous non radioactive cell proliferation assay, by Promega (3 mentions)
4.2 plus camera, by Olympus (1 mentions)
Ix83 inverted fluorescence microscope, by Olympus (1 mentions)
Ckk 8, by Merck Group (1 mentions)
Cck 8, by Thermo Fisher Scientific (1 mentions)
Cck 8 cell proliferation kit, by Dojindo Laboratories (1 mentions)
Ay303, by Sartorius (1 mentions)
Fz compact, by Labconco (1 mentions)
Cell counting kit 8 cck 8, by Merck Group (1 mentions)
8 protocols using multiskan go spectrometer
1
Microscopic Visualization of Diatom-Bacteria Interactions
2
Cytotoxicity of Pluronic-Stabilized MNPs
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Cells were plated at concentration of 10 000 cells per well in 96-well plates. The solutions of Pluronic F127 stabilized and uncovered MNPs in distilled water were added to the cells after one day (the final concentrations of magnetite are shown at the histogram (Fig. 7 )). Distilled water (20%) and DMSO (25%) were taken as negative and positive controls, correspondingly. Cells were incubated with these MNPs for 3 and 24 h. Then cells were washed with PBS, and 20 μl of MTS reagent (CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay, Promega, USA) was added to each well with 100 μl of culture medium. After 4 h incubation at 37 °C in darkness, the absorbance of the solution was measured at 490 nm using Thermo Scientific Multiskan GO spectrometer.
3
Assessing KDM2B Growth Potential in CRC
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To further assess the growth potential of KDM2B in CRC cells, cell proliferation and colony formation assays were employed. For the proliferation assay, 24 h after transfection, HT-29 and DLD-1 cells were seeded at a density of 3 × 103 cells per well in 96-well microplates in triplicates, and proliferative cells were stained with CKK-8 (Sigma-Aldrich, USA) and measured at 0, 24, 48, and 72 h. Two hours after adding 10 μl of CCK-8 reagent, the absorbance was measured at 450 nm using the Multiskan Go spectrometer (Thermo Fisher, USA). For the cell colony formation assay, 24 h post-transfection the cells mentioned above were harvested and seeded in 6-well plates at a density of 500 cells per well and incubated for 6 days for the formation of colonies. The colonies were fixed and stained with 1% crystal violet for 20 min and then counted.
4
Cytotoxicity of Iron Oxide Nanoparticles
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SC-1 cells were plated at concentration of 25,000 cells per well in 96-well plates. After 48 h, IONPs were added to the cells at final concentration 1–200 µg/mL. PBS and DMSO (25%) served as negative and positive controls, respectively. After 48 h incubation with IONPs, cells were washed with PBS, and 20 μL of MTS reagent (CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay, Promega, USA) was added to each well with 100 μL of culture medium. After 4 h incubation at 37°C in darkness, 100 μL of culture medium with MTS from each well was carefully replaced in new plates to avoid the presence of nanoparticles in the analyzed solution. The absorbance of the obtained solution was measured at 490 nm using Thermo Scientific Multiskan GO spectrometer. Experiments were performed in triplicates.
5
Sulf-1 Regulates Hca-F Cell Proliferation
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The effects of Sulf-1 up-regulation on Hca-F cell proliferation were measured using Dojindo's CCK-8 cell proliferation kit (Dojindo Molecular Technologies, Japan). Briefly, triplicates of 3× 103 cells/well of Hca-F, Sulf-1-Hca-F, and Nc-Hca-F cells were plated in 96 well plates and Cell proliferation was measured at 24hrs, 48hrs, 72hrs and 96hrs later. The Cell proliferation test was done by adding 10μl of CCK-8/well and the absorbance was measured 30min after adding the reagent at 450nm using Multiskan Go spectrometer (Thermofisher Scientific, USA).
6
Cyanobacterial Cell Cultivation and Analysis
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A 200 μL aliquot of the cell culture was collected and placed in a well of a 96-well microtiter plate. The absorption spectrum was measured from 250 to 830 nm using a Multiskan Go spectrometer (Thermo Fisher Co. Ltd., Tokyo, Japan). The cyanobacterial cells cultured in the 6 L tank were harvested via centrifugation and freeze-dried using a vacuum freeze dryer (FZ-Compact; Labconco Co. Ltd., Kansas City, USA). The dry cell weight was measured by using electric balance (AY303; Sartorius Co.Ltd., Göttingen, Germany).
7
Measuring HeLa Cell Proliferation by CCK-8
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The effects of TAZ on HeLa cell proliferation were measured by Cell Counting Kit-8 (CCK-8) (Sigma–Aldrich, USA). HeLa cells were plated at a density of 5 × 103 cells per well in 96-well plates 24 h after transfection. Thereafter, 10 µl CCK solution and 100 µl fresh medium were added to each well, and the plates were incubated in the dark at 37 °C for 2 h. After incubation, the absorbance at 452 nm was measured using a Multiskan Go spectrometer (Thermo Fisher, USA).
8
Evaluating AuFe NPs Cytotoxicity
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PC-3 and LNCaP cells were seeded in a 96-well plate (10 × 10 3 cells per well) in a culture medium and cultivated at 37 °C under a humidified atmosphere containing 5% CO 2 . The cells were counted using an automatic cell counter EVE. After 24 h, the cells were washed with 100 μL of serum-free medium and incubated with 100 μL of AuFe NP serial dilutions. To evaluate the cytotoxicity of NPs, the standard MTS test 39 was used. The solution of AuFe NPs in the concentration range 7.5-150.0 µg Au mL -1 was added to the cell culture medium. 48 h later, the cells were washed with 100 μL of serum-free medium and new 100 μL of culture medium with 20 μL of MTS reagent (CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay, Promega, USA) were added per well. After 4 h of incubation at 37 °C in darkness, the absorbance of the solution was measured at 490 nm using a Thermo Scientific Multiskan GO spectrometer. 1× PBS, 10% well volume, was used as a negative control, and 30% DMSO diluted in cell medium was used as a positive control.
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