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Uchl1

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UCHL1 is a recombinant protein that belongs to the family of deubiquitinating enzymes. It is involved in the regulation of the ubiquitin-proteasome pathway, which plays a crucial role in cellular protein homeostasis.

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Lab products found in correlation

Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions) Ebio64dec17, by Thermo Fisher Scientific (2 mentions) Sfci12t4d11, by Beckman Coulter (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Golgiplug, by BD (2 mentions) Rpa t8, by BD (1 mentions) Rpa t4, by BD (1 mentions) Rpmi 1640, by Merck Group (1 mentions)

Close spelling variants of this product

CD45RO (clone UCHL1), Clone UCHL1 Anti-CD45RO (clone UCHL1, CD45RO (UCHL1, FITC-conjugated anti-CD45RO (UCHL1; CD45RO-PE-Cy7 (clone UCHL1), CD45RO-PE (UCHL1, Anti CD45RO BV421 (clone UCHL1 CD45RO-BV421 Clone UCHL1 BV650 anti-CD45RO (clone UCHL1,

3 protocols using uchl1

1

Comprehensive Analysis of Cytokine Expression

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One million freshly isolated PBMCs were incubated overnight at 37°C, 5% CO2 in Iscove's Modified Dulbecco's Medium (GIBCO) supplemented with 10 % fetal calf serum (GIBCO) and 1% Penicillin/Streptomycin (GIBCO) in a 96-well plate. Cell were then treated with GolgiPlug (BD) to inhibit intracellular protein transport and stimulated with IL-2, PMA (0.05μg/ml) and Ionomycin (1μg/ml) for 4 hours at 37°C, 5% CO2. Subsequently, cells were stained for surface markers CD3 (SK7, BD), CD4 (SFCI12T4D11, Beckman Coulter) and CD45RO (UCHL1, BD) and intracellular markers IL17 (eBio64DEC17, eBioscience), IL-4 (8D4-8, eBioscience) and IFN-γ (B27, BD) and measured by flow cytometry. CD3+CD4+CD45RO+ cells were analyzed for cytokine expression levels.
Schubert D., Bode C., Kenefeck R., Hou T.Z., Wing J.B., Kennedy A., Bulashevska A., Petersen B.S., Schäffer A.A., Grüning B.A., Unger S., Frede N., Baumann U., Witte T., Schmidt R.E., Dueckers G., Niehues T., Seneviratne S., Kanariou M., Speckmann C., Ehl S., Rensing-Ehl A., Warnatz K., Rakhmanov M., Thimme R., Hasselblatt P., Emmerich F., Cathomen T., Backofen R., Fisch P., Seidl M., May A., Schmitt-Graeff A., Ikemizu S., Salzer U., Franke A., Sakaguchi S., Walker L.S., Sansom D.M, & Grimbacher B. (2014). Autosomal-dominant immune dysregulation syndrome in humans with CTLA4 mutations. Nature medicine, 20(12), 1410-1416.
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2

Comprehensive Analysis of Cytokine Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
One million freshly isolated PBMCs were incubated overnight at 37°C, 5% CO2 in Iscove's Modified Dulbecco's Medium (GIBCO) supplemented with 10 % fetal calf serum (GIBCO) and 1% Penicillin/Streptomycin (GIBCO) in a 96-well plate. Cell were then treated with GolgiPlug (BD) to inhibit intracellular protein transport and stimulated with IL-2, PMA (0.05μg/ml) and Ionomycin (1μg/ml) for 4 hours at 37°C, 5% CO2. Subsequently, cells were stained for surface markers CD3 (SK7, BD), CD4 (SFCI12T4D11, Beckman Coulter) and CD45RO (UCHL1, BD) and intracellular markers IL17 (eBio64DEC17, eBioscience), IL-4 (8D4-8, eBioscience) and IFN-γ (B27, BD) and measured by flow cytometry. CD3+CD4+CD45RO+ cells were analyzed for cytokine expression levels.
Schubert D., Bode C., Kenefeck R., Hou T.Z., Wing J.B., Kennedy A., Bulashevska A., Petersen B.S., Schäffer A.A., Grüning B.A., Unger S., Frede N., Baumann U., Witte T., Schmidt R.E., Dueckers G., Niehues T., Seneviratne S., Kanariou M., Speckmann C., Ehl S., Rensing-Ehl A., Warnatz K., Rakhmanov M., Thimme R., Hasselblatt P., Emmerich F., Cathomen T., Backofen R., Fisch P., Seidl M., May A., Schmitt-Graeff A., Ikemizu S., Salzer U., Franke A., Sakaguchi S., Walker L.S., Sansom D.M, & Grimbacher B. (2014). Autosomal-dominant immune dysregulation syndrome in humans with CTLA4 mutations. Nature medicine, 20(12), 1410-1416.
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3

Murine Erythroleukemia Cell Line FBL as a Model for Cancer Immunotherapy

Cited 1 time
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FBL is a murine erythroleukemia cell line of B6 origin that expresses the H-2b-restricted Friend murine leukemia virus-derived Gag glycoprotein as a tumor associated antigen [9] (link), [28] . Gag peptide (CCLCLTVFL) and ovalbumin (SIINFEKL) control peptides were purchased from Pi Proteomics. Cell culture was conducted in complete RPMI-1640 with 10% FBS (Sigma). Fluorochrome-conjugated antibody to mouse CD8 (53–6.7) was purchased from eBiosciences. Fluorochrome-conjugated mouse antibodies to CD90.1 (OX-7), IFNγ (XMG1.2), TNFα (MP6-XT22), and human antibodies to CD4 (RPA-T4), CD8 (RPA-T8), and CD45RO (UCHL1) were purchased from BD Biosciences. Fluorochrome conjugated antibodies to mouse neuropilin-1 (761705) and human neuropilin-1 (446921) were purchased from R&D Systems. efluor-670 cell dye was purchased from Invitrogen. Anti-mouse Nrp-1A blocking antibody was provided by Genentech and administered intraperitoneally at 10 mg/kg every other day, similar to studies previously described [45] (link).
Jackson S.R., Berrien-Elliott M., Yuan J., Hsueh E.C, & Teague R.M. (2014). Neuropilin-1 Expression Is Induced on Tolerant Self-Reactive CD8+ T Cells but Is Dispensable for the Tolerant Phenotype. PLoS ONE, 9(10), e110707.
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