Depletion was performed by two i.p. injection at day −2 and −1 of 200 μg of purified monoclonal rat anti-mouse CD4 Ab (Clone GK1.5, Bioxcell). Control (nondepleted) mice were treated similarly with rat IgG2b isotype (Clone LTF-2, Bioxcell). CD4+ T cell depletion in the spleen was assessed by flow cytometry.
Rat igg2b isotype
Manufactured by BioXCell
Rat IgG2b isotype is an immunoglobulin class and subclass found in rats. It is a commonly used isotype control for flow cytometry and immunoassays.
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Lab products found in correlation
2 protocols using rat igg2b isotype
1
CD4+ T Cell Depletion Protocol
2
Immune Cell Depletion and Neutralization
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Mice were injected i.p. with 250 µg CD4 (clone: GK1.5; Bio X Cell) and Gr-1 (clone: RB6-8C5; Bio X Cell). Injections were performed once a week for 4 wk starting 1 wk before the beginning of TPA treatment. For neutralization of CCL4 and IL17A, 100 µg CCL4 (R&D Systems)- or 200 µg IL-17A (clone: 17F3; Bio X Cell)–specific monoclonal antibodies were used. Control groups in CD4 and Gr-1 depletion and neutralization experiments received rat IgG2b isotype (clone: LTF2; Bio X Cell); controls for IL17A neutralization experiments received mouse IgG (Sigma-Aldrich), whereas controls in CCL4 neutralization experiments received goat IgG (R&D Systems).
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