We used collagen type I rat tail (3.52 mg/mL solution, BD Biosciences), chitosan (low molecular weight, Sigma-Aldrich), genipin (Challenge Bioproducts Co., 98%), acetic acid (Chempur), ethanol 96% (POOH), iron(III) chloride hexahydrate and iron(II) chloride tetrahydrate (Sigma-Aldrich), and ammonia (25% solution, puriss. p.a) (Sigma)
Iron 3 chloride hexahydrate and iron 2 chloride tetrahydrate
Manufactured by Merck Group
Iron(III) chloride hexahydrate and iron(II) chloride tetrahydrate are inorganic chemical compounds commonly used in various laboratory applications. Iron(III) chloride hexahydrate has the chemical formula FeCl3·6H2O, while iron(II) chloride tetrahydrate has the formula FeCl2·4H2O. These compounds are typically used as reagents, catalysts, and precursors in a range of scientific and industrial processes.
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2 protocols using iron 3 chloride hexahydrate and iron 2 chloride tetrahydrate
1
Collagen-Chitosan Composite Hydrogel
2
Gadolinium-Labeled Iron Oxide Nanoparticles
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Iron(III) chloride hexahydrate and iron(II) chloride tetrahydrate (Sigma), ammonia (25 % solution, puriss. p.a) (Sigma), Diethylenetriaminepentaacetic acid (DTPA, for complexometry >99.0 %, Fluka), N-Hydroxysuccinimide (NHS, 98 %, Aldrich), Gadolinium(III) chloride hexahydrate 99.999 % (Aldrich), N,N,N,N-Tetramethylethylenediamine >99 % (TEMED, BioReagent/SIGMA), N-(3-Dimethylaminopropylethylcarbodiimide hydrochloride crystalline (EDC, Sigma-Aldrich), Diethylenetriaminepentaacetic acid gadolinium(III) dihydrogen salt hydrate 97 % (GdDTPA, Aldrich), FeREXTM 10 mg Fe/mL, Ocean NanoTech LCC iron oxide nanocrystals (Ocean) 5 mg/mL, chitosan (low molecular weight, Aldrich). Deionized water was used for preparation of all the solutions.
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