Single cell suspension passed through 40 um (FisherBrand) and dead cell removal MS column (Miltenyi Biotec) were stained with anti-CD45 BV650 antibody (1:100, Biolegend), anti-CD34 PE antibody (1:100, Biolegend), anti-CD140 PE-Cy7 (1; 100, Biolegend), anti-SCA1 BV421 (1:100, Biolegend) in PBS containing 0.1% BSA at 4°C for 30 min. After 3 times of washing, Cytox green (1:100, Invitrogen) was added as a dead cell marker. Live CD140+ SCA1+ and CD140+SCA− cells from CD45−CD34+ populations were sorted on Aria II (BD biosciences), and the RNA were isolated using Trizol in combination with miRNeasy kit (Qiagen). 15 ul of elution buffer was used to elute RNA, and we performed qRT-PCR using RNA to Ct kit on Quant Studio 7 (Thermo Scientific) to detect gene differentially expressed in CD140+SCA1+ and CD140+SCA1−.
Anti cd34 pe antibody
Manufactured by BioLegend
The Anti-CD34 PE antibody is a fluorescently labeled monoclonal antibody that binds to the CD34 antigen, a transmembrane glycoprotein expressed on the surface of hematopoietic stem and progenitor cells. This antibody can be used for the identification and enumeration of CD34+ cells in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Mirneasy kit, by Qiagen (2 mentions)
Quantstudio 7, by Thermo Fisher Scientific (2 mentions)
Aria 2, by BD (2 mentions)
Cytox green, by Thermo Fisher Scientific (2 mentions)
Dead cell removal ms column, by Miltenyi Biotec (2 mentions)
Anti cd45 bv650 antibody, by BioLegend (2 mentions)
Anti cd140 pe cy7, by BioLegend (2 mentions)
Anti sca1 bv421, by BioLegend (2 mentions)
2 protocols using anti cd34 pe antibody
1
Sorting and Analyzing Mesenchymal Progenitors
2
Sorting and Analyzing Mesenchymal Progenitors
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Single cell suspension passed through 40 um (FisherBrand) and dead cell removal MS column (Miltenyi Biotec) were stained with anti-CD45 BV650 antibody (1:100, Biolegend), anti-CD34 PE antibody (1:100, Biolegend), anti-CD140 PE-Cy7 (1; 100, Biolegend), anti-SCA1 BV421 (1:100, Biolegend) in PBS containing 0.1% BSA at 4°C for 30 min. After 3 times of washing, Cytox green (1:100, Invitrogen) was added as a dead cell marker. Live CD140+ SCA1+ and CD140+SCA− cells from CD45−CD34+ populations were sorted on Aria II (BD biosciences), and the RNA were isolated using Trizol in combination with miRNeasy kit (Qiagen). 15 ul of elution buffer was used to elute RNA, and we performed qRT-PCR using RNA to Ct kit on Quant Studio 7 (Thermo Scientific) to detect gene differentially expressed in CD140+SCA1+ and CD140+SCA1−.
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