Anti odc1

Total proteins were isolated using RIPA lysis buffer (FD009, Fdbio science) and heated at 100 °C for 10 min. The protein from each group was separated by 12% SDS–PAGE and then transferred to polyvinylidene fluoride membranes (Roche, USA) for immunoblotting with the following primary antibodies: anti-HIGD1A antibody (Proteintech, 21749-1-AP), anti-HIF-1α antibody (BD Pharmingen, 610958), anti-DNMT1 antibody (Zen BioScience, R381634), anti-N-cadherin antibody (Affinity, AF4039-50), anti-E-cadherin antibody (Cell Signaling Technology, 3195T), anti-c-Myc antibody (Zen BioScience, 43250), anti-ODC1 (Proteintech, 28728-1-AP), anti-flag (Cell Signaling Technology, 2368S), anti-caspase3 (Cell Signaling Technology, 9662), anti-cleaved-caspase3 (Cell Signaling Technology, 9664), and anti-β-actin antibody (Cell Signaling Technology, 4970) at 1:1000 dilution. After blocking, the membrane was incubated with specific primary antibodies at 4 °C overnight. Subsequently, the membranes were probed with horseradish peroxidase-conjugated appropriate secondary antibody for 1 h at room temperature. Finally, the target bands were visualized using the ECL prime Western blotting detection reagent (GE Healthcare, USA) and detected with ImageQuant LAS 4000mini (GE Healthcare).