Lsm 880 2 airyscan fast confocal microscope

Manufactured by Zeiss

The LSM 880 II Airyscan FAST confocal microscope is a high-performance imaging system designed for advanced 3D and 4D microscopy. It features the Airyscan detector technology, which provides enhanced resolution and sensitivity compared to traditional confocal microscopes. The LSM 880 II Airyscan FAST is capable of fast data acquisition and offers a wide range of imaging modalities to support a variety of scientific applications.

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Lab products found in correlation

Ix83 microscope, by Olympus (1 mentions) Zen microscope software, by Zeiss (1 mentions) Metamorph software, by Molecular Devices (1 mentions) Axiocam 506 mono, by Zeiss (1 mentions) Axio observer z1 microscope, by Zeiss (1 mentions) Anti rabbit and anti mouse secondary antibodies, by Jackson ImmunoResearch (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Anti cd68, by Bio-Rad (1 mentions) Anti mouse and anti rabbit igg, by Novus Biologicals (1 mentions) Anti ccr2, by Novus Biologicals (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Eclipse 80i fluorescent microscope, by Nikon (1 mentions) Lsm 880 confocal microscope, by Zeiss (1 mentions) Smz18 fluorescent dissecting microscope, by Nikon (1 mentions)

Close spelling variants of this product

LSM 880 (3798 citations) LSM 880 confocal microscope (1702 citations) LSM 880 microscope (447 citations) LSM 880 Airyscan (196 citations) 880 confocal microscope (162 citations) LSM 880 Airyscan confocal microscope (154 citations) LSM 880 Airyscan microscope (76 citations) Airyscan confocal microscope (64 citations) LSM 880 confocal (43 citations) 880 Airyscan (36 citations)

5 protocols using lsm 880 2 airyscan fast confocal microscope

Multimodal Imaging of Cellular Structures

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Device imaging was performed using inverted fluorescent microscopy (Olympus IX83 microscope, Olympus; Tokyo, Japan) with MetaMorph software (Molecular Devices; San Jose, CA), with the following exceptions: Growth factor titration, KI-67, CC3, and UEA-1 experiments were imaged using a Zeiss Axio Observer.Z1 microscope with a Zeiss Axiocam 506 mono camera (Carl Zeiss AG; Oberkochen, Germany). The integrated GOC model was imaged using a Zeiss LSM 880 II Airyscan FAST confocal microscope with the accompanying Zeiss Zen Microscope software, as was the EC only bead perfusion study. DAPI staining of HIEC monolayers on Transwells was imaged using a Leica SP8X tandem scanning confocal microscope with a white light laser 40x oil objective, with LASX by Leica Microsystems software (Leica Microsystems; Wetzlar, Germany).

Seiler K.M., Bajinting A., Alvarado D.M., Traore M.A., Binkley M.M., Goo W.H., Lanik W.E., Ou J., Ismail U., Iticovici M., King C.R., VanDussen K.L., Swietlicki E.A., Gazit V., Guo J., Luke C.J., Stappenbeck T., Ciorba M.A., George S.C., Meacham J.M., Rubin D.C., Good M, & Warner B.W. (2020). Patient-derived small intestinal myofibroblasts direct perfused, physiologically responsive capillary development in a microfluidic Gut-on-a-Chip Model. Scientific Reports, 10, 3842.

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Immunofluorescent Imaging of Rat Aortic Cells

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After harvest, whole rat aortas were washed in PBS and then fixed in 30% sucrose mixed with 4% PFA for 2–3 h. Tissues were then blocked in 1% BSA with 1% Triton X-100 for 4 h to prevent nonspecific binding, and whole tissues were then incubated for 48 h at 4°C in primary antibody (anti-CCR2, Novus Bio, 1:50 and anti-CD68, Bio-Rad, 1:100) or control IgG (anti-mouse and anti-rabbit IgG, Novus Biologicals, 1:400). Anti-rabbit and anti-mouse secondary antibodies were applied (Jackson Laboratories) for 24 h at 4°C, and tissues were washed in PBS and incubated with DAPI (1:700 in PBS from Sigma). Tissues were then cleared in 70% ethanol, followed by 100% ethanol overnight. Whole tissues were then mounted in aqueous mounting media and imaged using a Zeiss LSM 880 II Airyscan FAST confocal microscope.

English S.J., Sastriques S.E., Detering L., Sultan D., Luehmann H., Arif B., Heo G.S., Zhang X., Laforest R., Zheng J., Lin C.Y., Gropler R.J, & Liu Y. (2020). CCR2 positron emission tomography for the assessment of abdominal aortic aneurysm inflammation and rupture prediction. Circulation. Cardiovascular imaging, 13(3), e009889.

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Assessing MAPT p.R406W Impacts on Endolysosomal Pathway

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To evaluate the impact of MAPT p.R406W on the endolysosomal pathway, culture media was aspirated, and cells were washed and fixed with 4% paraformaldehyde (Sigma, St Louis, MO, USA). Cells were washed and permeabilized with permeabilization buffer (0.1% Triton X-100 in PBS). Cells were then blocked in 3% bovine serum albumin (BSA; Sigma, St Louis, MO, USA) and incubated with primary and secondary antibodies diluted in 1% BSA. Immunostained cells were then imaged (Nikon Eclipse 80i fluorescent microscope) and acquired by using Metamorph Molecular Devices software. Confocal images were acquired by using Zeiss LSM 880 II Airyscan FAST Confocal Microscope.

Mahali S., Martinez R., King M., Verbeck A., Harari O., Benitez B.A., Horie K., Sato C., Temple S, & Karch C.M. (2022). Defective proteostasis in induced pluripotent stem cell models of frontotemporal lobar degeneration. Translational Psychiatry, 12, 508.

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Microglia Morphology Analysis in Dentate Gyrus

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Z-stacks (20 μm) of Iba1- labeled immunofluorescence images were acquired on an LSM 880 II Airyscan FAST confocal microscope (Zeiss) with a 60X objective, 1.8X zoom and 1,024 X 1,024 pixel resolution, For each mouse, a total of four z-stacks of the dentate gyrus region were taken from two separate brain sections. Microglia morphology analysis was performed on 3D images using Imaris 9.5 software (Bitplane). Morphology was analyzed using the Filament Tracer, with no loops allowed and spot detection mode to determine process start and end points per cell. Process reconstruction was made using the following custom settings: ‘detect new starting points’; ‘largest diameter 9.00 μm’, ‘seed points 2.00 μm’; ‘remove seed points around starting points’; ‘diameter of sphere regions: 15 μm’. All filament parameters were exported into separate Excel files and used for analyzing the number of process branches, process length, and process volume per cell. Image processing, three-dimensional reconstruction, and data analysis were performed in a blinded manner with regards to the experimental conditions.

Seo D.O., O’Donnell D., Jain N., Ulrich J.D., Herz J., Li Y., Lemieux M., Cheng J., Hu H., Serrano J.R., Bao X., Franke E., Karlsson M., Meier M., Deng S., Desai C., Dodiya H., Lelwala-Guruge J., Handley S.A., Kipnis J., Sisodia S.S., Gordon J.I, & Holtzman D.M. (2023). ApoE isoform and microbiota-dependent progression of neurodegeneration in a mouse model of tauopathy. Science (New York, N.Y.), 379(6628), eadd1236.

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Visualizing Schwann Cells in Zebrafish

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One-cell stage zebrafish embryos were injected with ~ 15–20 ng of sox10:Lifeact-RFP (a gift from the Lyons lab, University of Edinburgh) and 25 ng of transposase mRNA. 1 dpf larvae were then screened for expression of sox10:Lifeact-RFP in Schwann cells at 24 hpf. For live-imaging, larvae were anesthetized in Tricaine and embedded in 0.8% agarose on a 35 mm glass bottom dish filled with 0.2% Tricaine and covered with a 22 × 22 mm² coverslip on top of vacuum grease [40 (link)]. The larvae were then imaged with a Zeiss LSM 880 confocal microscope at 20× for 3 h at 3 min intervals. Still images were captured with a Zeiss LSM 880 II Airyscan FAST confocal microscope at 40xW with a 1.8 zoom. To examine blood vesssls, 4 dpf larvae with Tg(kdlr:mcherry) were imaged at 13.5× with a Nikon SMZ18 fluorescent dissecting microscope.

Cunningham R.L., Herbert A.L., Harty B.L., Ackerman S.D, & Monk K.R. (2018). Mutations in dock1 disrupt early Schwann cell development. Neural Development, 13, 17.

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