Troleandomycin

Manufactured by Merck Group

Sourced in United States

Troleandomycin is a lab equipment product manufactured by Merck Group. It is an antibiotic agent used in research and laboratory settings.

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Lab products found in correlation

4 protocols using troleandomycin

Cytotoxicity and CYP3A4 Enzymatic Assays

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Human hepatoma HuH-7 cells were purchased from Health Science Research Resources Bank, Japan Health Sciences Foundation (Osaka, Japan). HepG2/C3A cells were obtained from ATCC (Manassas, VA). HepaRG cells were purchased from Life Technologies (Grand Island, NY). Cell culture medium and medium supplements were obtained from Life Technologies. Fetal bovine serum was purchased from Atlanta Biologicals (Lawrenceville, GA). The following compounds (all ≥ 97% purity) were obtained from Sigma-Aldrich (St. Louis, MO): dimethyl sulfoxide (DMSO), ketoconazole, nifedipine, troleandomycin, rifampicin, dexathemasone, nordihydroguaiaretic acid (NDGA), nitrofurantoin, desipramine hydrochloride, troglitazone, nefazodone hydrochloride, bicinchoninic acid, and copper sulfate. P450-Glo CYP3A4 assay (luciferin-IPA substrate), CellTiter-Fluor, and CellTiter-Glo cell viability assays were purchased from Promega (Madison, WI). Supersomes human CYP3A4 plus reductase and b5, NADPH regenerating system, and rat tail collagen type I were obtained from Corning (Corning, NY). RNeasy mini Kit and QIA shredder were purchased from QIAGEN (Valencia, CA). High-capacity cDNA reverse transcription kits, TaqMan gene expression assays (beta-actin, 18S and human CYP3A4), and NucRed Live 647 fluorescent dye were purchased from Life Technologies.

Liu Y., Flynn T.J., Xia M., Wiesenfeld P.L, & Ferguson M.S. (2015). Evaluation of CYP3A4 inhibition and hepatotoxicity using DMSO-treated human hepatoma HuH-7 cells. Cell biology and toxicology, 31(4-5), 221-230.

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Synthesis and Characterization of NCMN and NCHN

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N-(3-carboxy propyl)-4-methoxy-1,8-naphthalimide (NCMN) and N-(3-carboxy propyl)-4-hydroxy-1,8-naphthalimide (NCHN) were synthesized by our laboratory with purity above 98% identified by HPLC-UV. 1-Aminobenzotriazole (ABT), sulfaphenazole, troleandomycin, furafylline, glucose-6-phosphate dehydrogenase, NADP+ and d-glucose-6-phosphate were purchased from Sigma (St. Louis, MO, USA). All other reagents were reagent grade or better. Pooled human liver microsomes (HLMs, from 50 donors, lot no. X008067) were obtained from Bioreclamation IVT (Baltimore, MD, USA). Liver microsomes form male ICR/CD-1 mouse (Lot. STOM, n = 500), male Sprague-Dawley rat (Lot.JPXY, n = 100), male beagle dog (Lot.DMXD, n = 3), male Yucatan minipig (Lot.GGSK, n = 2), male cynomolgus monkey (Lot.CYJC, n = 3) as well as cDNA-expressed rat CYP1A2 (Lot.int066e1c) were purchased from Research Institute for Liver Diseases (RILD, Shanghai, China).

Dai Z., Sun G., Yang J., Hou J., Zhou P., Xie W., Ge G., Sun X, & Yang L. (2019). Interspecies Variation in NCMN-O-Demethylation in Liver Microsomes from Various Species. Molecules, 24(15), 2765.

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Cytochrome P450 Inhibition Assay Protocol

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Flucloxacillin (CAS: 1847‐24‐1) was a kind gift of Dr Bayliss from the University of Liverpool. β‐Nicotinamide adenine dinucleotide phosphate disodium salt (NADP; CAS: 24292‐60‐2), glucose‐6‐phosphate (G‐6‐P; CAS: 54010‐71‐8), G‐6‐P dehydrogenase from baker's yeast (

Saccharomyces cerevisiae

; CAS: 9001‐40‐5), (+)‐N‐3‐benzyl‐nirvanol (CAS: 790676‐40‐3), bufuralol (CAS: 60398‐91‐6), chlorzoxazone (CAS: 95‐25‐0), diethyldithiocarbamate (CAS: 20624‐25‐3), fluvoxamine maleate (CAS: 22916‐47‐8), ketoconazole (CAS: 65277‐42‐1), (S)‐mephenytoin (CAS: 70989‐04‐7), miconazole (CAS: 22916‐47‐8), α‐naphtoflavone (CAS: 604‐59‐1), quercetin (CAS: 6151‐25‐3), quinidine (CAS: 56‐54‐2), sulfaphenazole (CAS: 526‐08‐9), testosterone (CAS: 58‐22‐0), ticlopidine (CAS: 53885‐35‐1) and troleandomycin (CAS: 2751‐09‐9) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Midazolam (CAS: 59467‐96‐8) was from Duchefa Farma (Haarlem, the Netherlands). All other chemicals were of analytical grade purity and obtained from standard suppliers.

Dekker S.J., Dohmen F., Vermeulen N.P, & Commandeur J.N. (2018). Characterization of kinetics of human cytochrome P450s involved in bioactivation of flucloxacillin: inhibition of CYP3A‐catalysed hydroxylation by sulfaphenazole. British Journal of Pharmacology, 176(3), 466-477.

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Recombinant Human P450 Enzyme Assay

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Bactosomes prepared from Escherichia coli cells coexpressing recombinant human NADPH-P450 reductase and individual human P450s (CYP1A2, 2C9, 2C19, 2D6 and 3A4) were purchased from CYPEX (Dundee, UK). 3-Cyano-7-ethoxycoumarin, sulfaphenazole, ketoconazole, quinidine, troleandomycin, caffeine and NADPH were obtained from Sigma-Aldrich (St. Louis, MO). 7-Benzyloxy-4-trifluoromethylcoumarin, 7-methoxy-4-trifluoromethylcoumarin, and 7-methoxy-4aminomethylcoumarin and PAMPA were obtained from BD Gentest (Woburn, MA). Cimetidine was obtained from Research Institute of Smithkline and French Laboratories Ltd.

Awortwe C., Bouic P.J., Masimirembwa C.M, & Rosenkranz B. (2014). Inhibition of Major Drug Metabolizing CYPs by Common Herbal Medicines used by HIV/AIDS Patients in Africa– Implications for Herb-Drug Interactions. Drug metabolism letters, 7(2), 83-95.

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