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2 protocols using mouse anti plcβ

1

Contractile Signaling Pathway Modulators

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Carbachol, acetylcholine, bradykinin, histamine, ionomycin, thapsigargin, bovine serum albumin (BSA), E-64, PDGF-BB, PMSF, collagen IV, fibronectin, staurosporine and Triton X-100 were purchased from Sigma. Collagen I, and III were purchased from BD Biosciences. ROCK inhibitor Y27632 was from EMD Millipore, and Collagenase D was from Roche. Methanolic Texas Red-conjugated phalloidin was obtained from Molecular Probes. Antibodies used in this study were purchased from the following sources: mouse anti-β−actin and mouse anti-vinculin (Sigma), mouse anti-GAPDH, rabbit anti-calponin, rabbit anti-SM22 and rabbit anti-B2R (Abcam), mouse anti-PLCβ (BD Biosciences), rabbit anti-ROCK1, rabbit anti-FAK, rabbit anti-pFAKY397 (Cell Signaling), mouse anti-Rac1 and mouse anti-RhoA (Millipore).
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2

Contractile Signaling Pathway Modulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Carbachol, acetylcholine, bradykinin, histamine, ionomycin, thapsigargin, bovine serum albumin (BSA), E-64, PDGF-BB, PMSF, collagen IV, fibronectin, staurosporine and Triton X-100 were purchased from Sigma. Collagen I, and III were purchased from BD Biosciences. ROCK inhibitor Y27632 was from EMD Millipore, and Collagenase D was from Roche. Methanolic Texas Red-conjugated phalloidin was obtained from Molecular Probes. Antibodies used in this study were purchased from the following sources: mouse anti-β−actin and mouse anti-vinculin (Sigma), mouse anti-GAPDH, rabbit anti-calponin, rabbit anti-SM22 and rabbit anti-B2R (Abcam), mouse anti-PLCβ (BD Biosciences), rabbit anti-ROCK1, rabbit anti-FAK, rabbit anti-pFAKY397 (Cell Signaling), mouse anti-Rac1 and mouse anti-RhoA (Millipore).
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