Dissolved iron concentration was determined using ICP-MS using a Perkin Elmer Optima 4300 (Perkin Elmer, Madrid, Spain) DV (Dual Vision) after acidulating the samples with 2% of nitric acid.
Optima 4300
Manufactured by PerkinElmer
Sourced in United States
The Optima 4300 is a versatile inductively coupled plasma optical emission spectrometer (ICP-OES) designed for elemental analysis. It provides high-performance multi-element analysis capabilities for a wide range of sample types and applications.
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10 protocols using optima 4300
1
Dissolved Iron Concentration by ICP-MS
2
Synthesis of Bi4C@US-Tubes for In Vitro Studies
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Bi4C@US-tubes were prepared as previously reported [56 (link)]. Briefly, the US-tubes and the Bi(III) oxo-salicylate cluster were prepared separately and then combined by simply sonicating both together for 1 h in dried tetrahydrofuran (THF). The US-tubes were produced by cutting full-length single-walled carbon nanotubes (SWCNTs) by the fluorination method described elsewhere [57 (link)], and the synthesis of the Bi(III) oxo-salicylate cluster ([Bi4(μ3-O)2(HO-2-C6H4CO2)8]·2MeCN) was performed as previously reported [58 (link)] and placed under vacuum for 48 h for solvent removal. For in vitro studies, a labeling solution was prepared by suspending Bi4C@US-tubes in a 0.17% (w/v) solution of Pluronic® F-108, a nonionic surfactant, via probe sonication for 5 min. The samples were centrifuged at 3200 rpm for 10 min, and the supernatant was used for the in vitro experiments. The bismuth concentration was determined using inductively coupled plasma optical emission spectrometry (ICP-OES, Optima 4300 from PerkinElmer, Inc.) and adjusted to 1 μM.
3
Calcium Content Determination Protocol
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The calcium content were determined by mixing an aliquot (500 μl) of the sample with concentrated hydrochloric acid (500 μl) in a glass vial container and incubated at room temperature overnight. The sample was subsequently diluted 50-fold and the calcium content was determined employing a inductively-coupled plasma emission spectrophotometer (Optima 4300, Perkin Elmer, Waltham, MA). Calibration standard solution of calcium (37509–04 Nacalai Tesque) was used.
4
Characterizing AuNPs-modified Carbon Materials
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Transmission electron microscopic measurements (TEM) were carried out using JEOL TEM, JEM-2010 model, which is equipped with and Oxford X-ray detector (EDS), INCA Energy TEM 100 model, and GATAN acquisition camera.
X-Ray photoelectron spectroscopy (XPS) was performed in a VG-Microtech Mutilab 3,000 spectrometer and Al Kα radiation (1253.6 eV). The deconvolution of the XPS Au4f, C1s, S2p, and N1s was done by least squares fitting using Gaussian-Lorentzian curves, while a Shirley line was used for the background determination. The S2p spectra have been analyzed considering the spin-orbit splitting into S2p3/2 and S2p1/2 with a 2:1 peak area ratio and 1.2 eV splitting (Castner et al., 1996 (link)). The XPS measurements were done in different parts of a given sample and repeated in two different samples, being the results similar.
To determine metal content, 10 mg of the carbon material modified with AuNPs were digested in an acid solution [1 HNO3 (65%):3 HCl (37%)]. The suspension was sonicated for 20 min and heated at 80°C for 6 h until evaporation. Afterwards, 2 mL of HNO3 were added and diluted with ultrapure water. Solutions were then analyzed using inductively coupled plasma optical emission spectroscopy (ICP-OES), Perkin-Elmer Optima 4,300.
X-Ray photoelectron spectroscopy (XPS) was performed in a VG-Microtech Mutilab 3,000 spectrometer and Al Kα radiation (1253.6 eV). The deconvolution of the XPS Au4f, C1s, S2p, and N1s was done by least squares fitting using Gaussian-Lorentzian curves, while a Shirley line was used for the background determination. The S2p spectra have been analyzed considering the spin-orbit splitting into S2p3/2 and S2p1/2 with a 2:1 peak area ratio and 1.2 eV splitting (Castner et al., 1996 (link)). The XPS measurements were done in different parts of a given sample and repeated in two different samples, being the results similar.
To determine metal content, 10 mg of the carbon material modified with AuNPs were digested in an acid solution [1 HNO3 (65%):3 HCl (37%)]. The suspension was sonicated for 20 min and heated at 80°C for 6 h until evaporation. Afterwards, 2 mL of HNO3 were added and diluted with ultrapure water. Solutions were then analyzed using inductively coupled plasma optical emission spectroscopy (ICP-OES), Perkin-Elmer Optima 4,300.
5
Dietary Mineral Analysis and Intake
6
Elemental Analysis of Leaf Samples
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Leaf samples (0.5 g DW) were dry-ashed and dissolved in HCl according to Duque (1971) . Potassium, magnesium, calcium, phosphorus, manganese, iron, zinc and sodium concentrations were determined using a Perkin Elmer Optima 4300 inductively coupled plasma optical emission spectroscopy (ICP-OES) (Perkin Elmer, USA). The operating parameters of the ICP-OES were the following: radio frequency power 1,300 W, nebulizer flow 0.85 L • min -1 , nebulizer pressure 30 psi, auxiliary gas flow 0.2 L • min -1 , sample introduction 1 mL • min -1 .
7
Milk Composition Analysis Methods
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Total solids of milk samples were measured using AOAC International (2000) method 925.23. The total N and NPN were determined using the Kjeldahl method according to ISO 8968-1 (ISO-IDF, 2001a) and ISO 8968-4 (ISO-IDF, 2001b), respectively. The calcium and phosphorus content was measured by inductively coupled plasma-optical emission photometer (Optima 4300, dual view, Perkin-Elmer, Norwalk, CT) with wavelengths of 327.396 and 178.287 nm, respectively (Bazinet et al., 2000) .
8
Elemental Analysis of Leaf Samples
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Leaf samples (0.5 g DW) were dry-ashed and dissolved in HCl in accordance with Duque (1971) . Phosphorus, potassium, calcium, magnesium, manganese, zinc, iron and sodium concentrations were determined using a Perkin Elmer Optima 4300 inductively coupled plasma optical emission spectroscopy (ICP-OES) (Perkin Elmer, USA). The ICP-OES operating parameters were: radio frequency power 1300 W, nebulizer flow 0.85 L min -1 , nebulizer pressure 206.84 kPa, auxiliary gas flow 0.2 L min -1 , sample introduction 1 mL min -1 and three replicates per sample. Total nitrogen and carbon were quantified after leaf DW combustion (950°C) with pure oxygen using an elemental analyser with a thermal conductivity detector (TruSpec CN, Leco, USA).
9
Relaxation Properties of Gd-Loaded US-Tube CAs
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Relaxation properties of the Gd 3+ -ion-loaded US-tube CAs were measured using a Bruker Minispec (mq 60) benchtop relaxometer operating at 1.41 T and 37 °C. The longitudinal (T 1 ) relaxation times were obtained using an inversion recovery pulse sequence. Gd 3+ -ion concentration was determined by inductively-coupled plasma optical emission spectroscopy (ICP-OES, using a Perkin-Elmer Inc. Optima 4300 instrumentation). Samples were digested in 26% HClO 3 and reconstituted in 5 mL of trace metal-grade 2% HNO 3(aq) prior to data collection.
10
Multi-Elemental Analysis of Plant Bulbs
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For phosphorus, potassium, magnesium, calcium, manganese, iron, zinc and copper analyses, samples (0.5 g DM) of three bulbs per treatment were dry-ashed and dissolved in HCl according to Duque (1971) . Mineral concentrations were determined using a Perkin Elmer Optima 4300 inductively coupled plasma optical emission spectroscopy (ICP-OES) (Perkin Elmer, Massachusetts, USA) and standards were supplied by Merck KGaA (Darmstadt, Germany). The operating parameters of the ICP-OES were: radio frequency power, 1300 W; nebulizer flow, 0.85 L min -1 ; nebulizer pressure, 30 psi; auxiliary gas flow, 0.2 L min -1 ; sample introduction, 1 mL min -1 and three replicates per sample.
Carbon and nitrogen content was determined in bulb samples (n=5) previously dried at 60 :C over 48 h and weighed. One mg aliquots were weighed in small tin capsules and, C and N determinations were carried out with an Elemental Analyser (EA) (CarboErba, Milan, Italy).
Carbon and nitrogen content was determined in bulb samples (n=5) previously dried at 60 :C over 48 h and weighed. One mg aliquots were weighed in small tin capsules and, C and N determinations were carried out with an Elemental Analyser (EA) (CarboErba, Milan, Italy).
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