Smmc 7721

Human hepatoma cell lines Huh7 and SK-Hep1 were obtained directly from Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China)³⁴ (link), and SMMC-7721 and BEL-7404 were purchased from Procell Life Science&Technology Co., Ltd (Wuhan, China). Cells were maintained in Dulbecco’s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. All cultures were maintained in a humidified incubator at 37°C and 5% CO₂. The cell lines have been characterized at the cell bank by DNA fingerprinting analysis using short tandem repeat markers. All cell lines were placed under cryostage after they were obtained from the cell bank and used within 6 months of thawing fresh vials, as described previously.²¹ (link)^,34 (link)

The human normal liver cell line (L-02) and liver cancer cell lines (Huh7, HepG2, Hep3B, SMMC-7721, and SK-Hep-1) were purchased from Procell (Wuhan, China). L-02 and SMMC-7721 cells were maintained in RPMI 1640 (#11875119, Thermo Fisher, USA) supplemented with 5% FBS (#12303C, Sigma-Aldrich, USA) and 1% penicillin/streptomycin (#SV30010, GE Healthcare, USA). The remaining cells were cultured in DMEM (#10313021, Thermo Fisher, USA) containing 10% FBS and 1% penicillin/streptomycin. Cells were all incubated in a humidified 5% CO₂ incubator at 37°C.
HepG2 cells were transfected with miR-10b-5p mimics, mimics NC (GenePharma, China), oe-NC, or oe-SLC38A2 vector (HonorGene, China). SK-Hep-1 cells were transfected with miR-10b-5p inhibitors and inhibitor NC (GenePharma, China) using Lipofectamine 2000 (#11668019, Thermo Fisher, USA) according to the manufacturer’s guidelines. To induce apoptosis, SK-Hep-1 and HepG2 cells were treated with the apoptosis inducer staurosporine (STS, 5 μM, #S1421, Selleck, USA) for 12 h.