Osr6116

Manufactured by Beckman Coulter

Sourced in United States

The OSR6116 is a chemistry analyzer designed for automated in-vitro diagnostic testing of a variety of analytes in clinical laboratory settings. The device performs quantitative analysis of samples using spectrophotometric detection methods. Core functions include sample aspiration, reagent addition, incubation, and analyte measurement.

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Lab products found in correlation

Osr6133, by Beckman Coulter (3 mentions) Osr60118, by Beckman Coulter (3 mentions) One touch ultra blue, by LifeScan (2 mentions) Au400, by Beckman Coulter (2 mentions) Ultra sensitive mouse insulin, by Crystal Chem (2 mentions) Osr6187, by Beckman Coulter (2 mentions) Beckman au480 clinical, by Beckman Coulter (1 mentions) Au680 analyzer, by Beckman Coulter (1 mentions) Nefa hr 2 kit, by Fujifilm (1 mentions) Bicinchoninic acid assay, by Thermo Fisher Scientific (1 mentions) Osr6195, by Beckman Coulter (1 mentions) Au480, by Beckman Coulter (1 mentions) Osr6234, by Beckman Coulter (1 mentions) Nefa hr 2, by Fujifilm (1 mentions) Ultra sensitive mouse insulin elisa, by Crystal Chem (1 mentions)

8 protocols using osr6116

Fasting Blood Metabolite Analysis

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At the time of sacrifice after a 6-hour morning fast, whole blood glucose measurements were made by glucometer (One Touch Ultra Blue, LifeScan, Inc). Serum from blood obtained at necropsy was removed after of low speed centrifugation. Insulin was determined by ELISA (Ultra-sensitive mouse insulin, Crystal Chem, Inc), and FFA was determined using a colormetric kit (Wako Diagnostics). Total TAG (glycerol phosphate oxidase, Beckman Coulter OSR6133) and total cholesterol (Aminoantipyrine/Phenol/Peroxidase, Beckman Coulter OSR6116) by enzymatic colorimetric endpoint assay (Beckman Coulter AU400).

Killion E.A., Reeves A.R., El Azzouny M.A., Yan Q.W., Surujon D., Griffin J.D., Bowman T.A., Wang C., Matthan N.R., Klett E.L., Kong D., Newman J.W., Han X., Lee M.J., Coleman R.A, & Greenberg A.S. (2018). A role for long-chain acyl-CoA synthetase-4 (ACSL4) in diet-induced phospholipid remodeling and obesity-associated adipocyte dysfunction. Molecular Metabolism, 9, 43-56.

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Lipid Profile Measurement Protocol

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Plasma total triglycerides, total cholesterol, and high-density lipoprotein (HDL)-cholesterol concentrations were measured using commercial enzymatic kits (OSR60118, OSR6116, and OSR6187, respectively (Beckman Coulter Inc., Georgia, USA)) on a Beckman AU480 clinical analyser (Beckman Coulter Inc., Atlanta, GA, USA). Plasma low-density lipoprotein (LDL)-cholesterol concentrations were estimated using the Friedewald equation [24 (link)]:

O’Rielly R., Li H., Lim S.M., Yazbeck R., Kritas S., Ullrich S.S., Feinle-Bisset C., Heilbronn L, & Page A.J. (2020). The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice. Nutrients, 12(8), 2446.

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Plasma Biomarkers Measurement Protocol

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Aspartate aminotransferase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) were measured in plasma using diagnostic kits (OSR6009, 6007, and 6004, respectively; Beckman Coulter) adapted for the Olympus AT200 auto analyser. Plasma cholesterol and triacylglycerols were determined using diagnostic kits OSR6116, 61118, and OE66300 (Beckman Coulter).

Hongsibsong S., Stuetz W., Sus N., Prapamontol T., Grune T, & Frank J. (2014). Dietary exposure to continuous small doses of α-cypermethrin in the presence or absence of dietary curcumin does not induce oxidative stress in male Wistar rats. Toxicology Reports, 1, 1106-1114.

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Serum Total Cholesterol Determination

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Total cholesterol serum level was determined with an enzymatic method. A reagent set from Beckman Coulter (Cat. No. OSR 6116) was used for the determination, which was conducted according to the manufacturer’s instruction for Beckman Coulter AU 680 analyzer. Total cholesterol level was given in mg/dL.

Rzepecka-Stojko A., Stojko J., Jasik K, & Buszman E. (2017). Anti-Atherogenic Activity of Polyphenol-Rich Extract from Bee Pollen. Nutrients, 9(12), 1369.

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Quantification of Metabolic Biomarkers

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Whole blood glucose measurements were made by glucometer (One Touch Ultra Blue, LifeScan, Inc). Blood plasma was obtained by cardiac puncture. The plasma supernatant of low speed centrifugation was analyzed by ELISA for mouse insulin (Ultra-sensitive mouse insulin, Crystal Chem, Inc), and by enzymatic endpoint for β-hydroxybutyrate (β-hydroxybutyrate dehydrogenase, Stanbio), and enzymatic colorimetric endpoint assay (Beckman Coulter AU400) for triglyceride (glycerol phosphate oxidase, Beckman Coulter OSR6133) and cholesterol (Aminoantipyrine/Phenol/Peroxidase, Beckman Coulter OSR6116) content. Serum and liver free fatty acids were determined using the Wako NEFA HR(2) kit from serum and liver lipid from Folch 2:1 chloroform:methanol organic layer extract dried and resuspended in 1% Triton. Liver free fatty acids were normalized to protein content of aqueous layer by bicinchoninic acid assay (Thermo Scientific Pierce).

Bowman T.A., O'Keeffe K.R., D'Aquila T., Yan Q.W., Griffin J.D., Killion E.A., Salter D.M., Mashek D.G., Buhman K.K, & Greenberg A.S. (2016). Acyl CoA synthetase 5 (ACSL5) ablation in mice increases energy expenditure and insulin sensitivity and delays fat absorption. Molecular Metabolism, 5(3), 210-220.

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Serum Lipid Profiling in Mice

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Body weight was measured at the end of the special diet, before the mice were euthanized at the beginning of an experiment. Serum was obtained through a cardiac puncture after 24 h of starvation, centrifuged at 12,500 rpm at 4 °C, and frozen at −80 °C until measured. Total cholesterol (Beckman Coulter, OSR6116, Brea, CA, USA), triglycerides (Beckman Coulter, OSR60118), HDL (Beckman Coulter, OSR6195), and LDL (Beckman Coulter, OSR6196) were measured with an AU chemistry analyzer (Beckman Coulter, AU480) according to the protocol of the manufacturer.

Christ C., Ocskay Z., Kovács G, & Jakus Z. (2024). Characterization of Atherosclerotic Mice Reveals a Sex-Dependent Susceptibility to Plaque Calcification but No Major Changes in the Lymphatics in the Arterial Wall. International Journal of Molecular Sciences, 25(7), 4046.

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Comprehensive Serum Biomarker Analysis

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Serum CHOL, TG, HDL, LDL, urea (OSR60118, OSR6116, OSR6187, OSR6183, OSR6234, Beckman Coulter Inc., Georgia, USA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) (C010-2–1, C009-2–1, Nanjing Jiancheng Bioengineering Institute, China) were measured using commercial enzymatic kits on a Beckman AU480 chemistry analyzer (Beckman Coulter Inc., Atlanta, GA, USA).

Zhang Y., Wang Y., Liao X., Liu T., Yang F., Yang K., Zhou Z., Fu Y., Fu T., Sysa A., Chen X., Shen Y., Lyu J, & Zhao Q. (2024). Glutamine prevents high-fat diet-induced hepatic lipid accumulation in mice by modulating lipolysis and oxidative stress. Nutrition & Metabolism, 21, 12.

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Plasma Metabolic Profiling in Mice

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All plasma measures were obtained from samples collected at time of sacrifice as described above. Plasma insulin was determined via ELISA (Ultra-Sensitive Mouse Insulin ELISA, Crystal Chem, Zaandam, Netherlands). Triglyceride (OSR6133, Beckman Coulter, Galway, Ireland), cholesterol (OSR6116, Beckman Coulter), HDL (OSR6195, Beckman Coulter), β-hydroxybutyrate (LiquiColor, StanBio, Boerne, TX, USA), and non-esterified fatty acids (NEFA-HR (2), Wako Diagnostics) were determined calorimetrically.

Griffin J.D., Bejarano E., Wang X.D, & Greenberg A.S. (2021). Integrated Action of Autophagy and Adipose Tissue Triglyceride Lipase Ameliorates Diet-Induced Hepatic Steatosis in Liver-Specific PLIN2 Knockout Mice. Cells, 10(5), 1016.

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