Tecnai 12 transmission electron microscope

Manufactured by Olympus

The Tecnai 12 is a transmission electron microscope (TEM) designed and manufactured by Olympus. It is a scientific instrument used for high-resolution imaging and analysis of small-scale structures and materials. The Tecnai 12 provides users with the capability to study the morphology, composition, and atomic structure of specimens at the nanometer scale.

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Lab products found in correlation

Veleta 4 megapixel side mount ccd camera, by Olympus (2 mentions) Ultra 45, by Electron Microscopy Sciences (2 mentions) Megaview 3 digital camera, by Olympus (1 mentions) Uct ultramicrotome, by Leica (1 mentions)

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Tecnai transmission electron microscope Transmission electron microscope

3 protocols using tecnai 12 transmission electron microscope

Transmission Electron Microscopy Protocol

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For ultrastructural examination, representative or interesting areas were selected based on light-microscopical assessment. Ultrathin (60–90 nm) sections were cut with a diamond knife (Ultra 45°, DiATOME) on an Ultrotome III (LKB) and collected upon Pioloform-coated, single-slot copper grids. The sections were contrast-stained with 7% uranyl acetate in 70% methanol and Reynolds’ lead citrate solution and examined in a FEI Tecnai 12 transmission electron microscope (operating at 80 kV) equipped with a Veleta 4-megapixel side-mount CCD camera (Olympus Soft Imaging Solutions GmbH).
Digital images were acquired with TIA software (TEM Imaging & Analysis, version 4.7 SP3; FEI). Alternatively, sets of serial digital images with 10% overlap (at an original magnification of ×30,000) were acquired with the automated EM data acquisition software package SerialEM^{133 (link)} (https://bio3d.colorado.edu/SerialEM). Final alignment (stitching) of the sets of overlapping images and montage blending were done using the reconstruction and modeling software package IMOD^{123 (link)} (https://bio3d.colorado.edu/imod) operating under the Unix toolkit Cygwin. Montages or single images were finally saved as TIFF and imported into Fiji ImageJ for further image processing, examination and selection of interesting areas. Images were assembled into figures using Adobe Photoshop.

Valentin H.E., Mitsky T.A., Mahadeo D.A., Tran M, & Gruys K.J. (2000). Application of a Propionyl Coenzyme A Synthetase for Poly(3-Hydroxypropionate-co-3-Hydroxybutyrate) Accumulation in Recombinant Escherichia coli. Applied and Environmental Microbiology, 66(12), 5253-5258.

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Transmission Electron Microscopy Protocol

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van der Valk W.H., van Beelen E.S., Steinhart M.R., Nist-Lund C., Osorio D., de Groot J.C., Sun L., van Benthem P.P., Koehler K.R, & Locher H. (2023). A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs. Cell reports, 42(6), 112623.

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Indirect Immunolabeling of Ultrathin Cryosections

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HeLa cells were processed for indirect immunolabeling of ultrathin cryosections essentially as previously described (McCaffery and Farquhar, 1995 ). Briefly, cells were fixed in a monolayer at 4°C in 100 mM PO₄ (pH 7.4) and 2.5% sucrose and containing 4% formaldehyde. The cells were harvested, pelleted, and cryo-protected in 2.3 M sucrose containing 30% polyvinyl pyrollidone. Cell pellets were mounted onto aluminum cryopins and frozen in liquid nitrogen. Ultrathin cryosections were then cut on a Leica UCT ultramicrotome equipped with a fetal calf serum (FCS) cryostage, and sections were collected onto 300 mesh, formvar/carbon-coated nickel grids. Grids were washed, blocked in 10% FCS, and incubated overnight with primary chicken anti-GFP antibody (10 μg/ml). After being washed, grids were incubated with 6- or 12-nm Au-conjugated donkey anti-chicken antibody (Jackson ImmunoResearch Labs, Ft. Washington, PA) for 2 h, washed, and subsequently embedded in a mixture containing 3.2% polyvinyl alcohol (10,000 MW), 0.2% methyl cellulose (400 centipoises), and 0.2% uranyl acetate. Sections were analyzed on a Tecnai 12 transmission electron microscope and images collected with a Soft Imaging System Megaview III digital camera.

Odeh H.M., Coyaud E., Raught B, & Matunis M.J. (2018). The SUMO-specific isopeptidase SENP2 is targeted to intracellular membranes via a predicted N-terminal amphipathic α-helix. Molecular Biology of the Cell, 29(15), 1878-1890.

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