Acetonitrile hipersolv chromanorm lc ms grade

Ultrapure water used for extraction of samples, preparation of reagents and as HPLC mobile phase was Milli-Q grade produced by a Millipore system (Merck Millipore, Darmstadt, Germany). Acetonitrile HiPerSolv Chromanorm LC-MS grade was from VWR International (Leuven, Belgium). Reagents for the post-column derivatisation system were NaOH from Merck KGaA (Darmstadt, Germany), ammonium acetate ACS reag Ph Eur, EMSURE, from Merck and methyl acetoacetate > 99% from Acros Organics (Geel, Belgium).
Analytical standards of DON, DON-3-G, 3-AcDON and 15-AcDON were all Biopure solutions (in acetonitrile) supplied by Romer Labs Diagnostic GmbH (Tulln, Austria), except 15-AcDON which was purchased as the solid (98.8% purity).
Four brands of IACs were compared regarding their cross-reactivity and extraction efficiency: DONTest WB from Vicam, lot nr. 238 (Milford, MS, USA); Neocolumn for DON from Neogen Europe Ltd, batch nr. D1010813 (Auchincruive, UK); DONPrep from R-Biopharm Rhone Ltd, batch nr. Al922/50 (Glasgow, UK) and Immunoclean CF DON from Aokin (Berlin, Germany). Sample extracts were purified by IAC clean-up on a 12-port vacuum manifold from Alltech (Thermo Fisher Scientific Inc., Waltham, MA, USA). IAC columns were kept in the fridge at 4°C and allowed to reach room temperature before use.