HFLS-RA (Cell Applications, USA) was used for the in vitro experimental validation in the current study. The cells were cultured in sterile synoviocyte growth medium (Cell Applications, USA) supplemented with 100 U/mL 1 penicillin, 80 U/mL 1 streptomycin, 2 mM Gln-glutamine, and were maintained at 37°C in a humidified 5% CO2 incubator. HFLS–RA were used at passage numbers 4 to 8 in this study.
Sterile synoviocyte growth medium
Manufactured by Cell Applications
Sourced in United States
Sterile synoviocyte growth medium is a specialized cell culture media formulated to support the growth and maintenance of synoviocytes, which are cells found in the synovial membrane of joints. This medium provides the necessary nutrients and growth factors required for the in vitro cultivation of these cells.
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Lab products found in correlation
4 protocols using sterile synoviocyte growth medium
1
Synoviocyte Culture for RA Research
2
Validation of Macrophage and Synovial Fibroblast Cell Lines
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Mouse macrophage cell lines (Raw264.7) and immortalized cell lines of synovial fibroblasts from the articular cavity in RA patients (MH7A cells) were used for experiment validation in vitro. Raw264.7 macrophage cells were maintained in DMEM (Hyclone, Logan, UT, United States), supplemented with 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, United States), 100 U/mL penicillin, 100 μg/ml streptomycin, and 2 mML glutamine (TBD, Tianjin, China) in a humidified 5% CO2 incubator at the temperature of 37°C. The Raw264.7 macrophage cells of low passage number were used in the current experiment validations.
MH7A cells were cultured in a sterile synoviocyte growth medium (Cell Applications, Inc., San Diego, CA) added with 10% synoviocyte growth supplement (Cell Applications, Inc., San Diego, CA), 100 U/mL penicillin, 100 μg/ml streptomycin, and 2 mML glutamine (TBD, Tianjin, China) in a humidified 5% CO2 incubator at the temperature of 37°C. MH7A cells of passage numbers four to eight were used in the current experiment validations.
MH7A cells were cultured in a sterile synoviocyte growth medium (Cell Applications, Inc., San Diego, CA) added with 10% synoviocyte growth supplement (Cell Applications, Inc., San Diego, CA), 100 U/mL penicillin, 100 μg/ml streptomycin, and 2 mML glutamine (TBD, Tianjin, China) in a humidified 5% CO2 incubator at the temperature of 37°C. MH7A cells of passage numbers four to eight were used in the current experiment validations.
3
In Vitro Synoviocyte Culture
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In the current study, HFLS-RA (Cell Applications, San Diego, CA 92121, USA) were used for in vitro experimental validation. The cells were cultured in sterile synoviocyte growth medium (Cell Applications, San Diego, CA 92121, USA) containing 100 U/mL 1 penicillin, 80 U/mL 1 streptomycin, and 2 mM Gln-glutamine in a humidified atmosphere at 37°C in the presence of 5% CO2.
4
HFLS-RA Cells Incubated with IL-1β and GSZD
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HFLS-RA (Cell Applications, USA) cells were used for in vitro experimental validation. The cells were cultured in sterile synoviocyte growth medium (Cell Applications, USA) supplemented with 100 U/mL 1 penicillin, 80 U/mL 1 streptomycin, and 2 mM glutamine and were maintained at 37 °C in a humidified atmosphere of 5 % CO2/95 % air. The cells were used between passage 4 and 8 and were incubated with 10 ng/mL IL-1β and different concentrations of GSZD (5.12 × 10−5, 2.56 × 10−4 and 1.28 × 10−3 μg/mL) for 24 h.
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