A blood sample was collected in the morning after overnight fasting to measure the levels of fasting blood glucose (FBG), glycated haemoglobin (HbA1c), total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and triglycerides, using standard automated enzymatic colorimetric methods (AutoMate 2550, Beckmann Coulter, Brea, CA, USA) under strict quality control. LDL cholesterol was calculated using the Friedewald equation. Plasma glucose was determined using the glucose oxidase method (Sclavus, Siena, Italy). Blood cell count was determined by a Coulter haematology analyser (Beckman–Coulter, Brea, CA, USA). Serum FT3, FT4, and TSH were measured using a competitive photometric method based on the solid-phase antigen-linked technique (LIASON FT3, LIASON FT4, LIASON TSH, Dia-Sorin, Saluggia, Italy). Serum high-sensitivity C-reactive protein (CRP) was assayed using a latex particle-enhanced immunoturbidimetric assay (Kamiya Biomedical Company, Seattle, WA, USA) (reference range: 0–5.5 mg/L; inter-assay coefficient of variation: 4.5%). Serum 25(OH)D was quantified by a chemiluminescence method (Diasorin Inc., Stillwater, MN, USA), and all samples were analysed in duplicate.
Liason ft4
Manufactured by DiaSorin
Sourced in Italy
The LIASON FT4 is a diagnostic lab equipment product offered by DiaSorin. It is designed to measure free thyroxine (FT4) levels in patient samples. The LIASON FT4 uses a chemiluminescent immunoassay technology to perform this analysis.
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2 protocols using liason ft4
1
Standardized Cardiometabolic Biomarker Panel
2
Comprehensive Metabolic Panel for Clinical Research
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Blood samples were taken between 8 and 9 am after fasting overnight. Serum insulin concentrations were determined by radioimmunoassay (Behring, Scoppitto, Italy). Serum FT3, FT4, and TSH were measured using a competitive photometric method based on the solid phase antigen-linked technique (LIASON FT3, LIASON FT4, LIASON TSH, Dia-Sorin, Saluggia, Italy). Intact PTH determination was done by immune-chemiluminescence assay (Test LIAISON 1-84 PTH Dia-Sorin, Saluggia, Italy). Plasma glucose levels were determined by the glucose-oxidase method (Sclavo, Siena, Italy). Insulin resistance was calculated by the homeostasis model assessment (HOMAIR) [31 (link)]. Serum 25(OH)D was quantified by chemiluminescence method (Diasorin Inc, Stillwater, USA). The total (uncarboxylated and carboxylated) serum osteocalcin (OC) levels were determined by electrochemiluminescence using the Elecsys N-MID Osteocalcin kit (Roche Diagnostics, Mannheim, Germany), Modular Analytics E170 (Roche Diagnostics). Plasma lipids (triglycerides, total cholesterol and HDL-cholesterol) were determined by an automatic colorimetric method (Hitachi; Boehringer Mannheim, Mannheim, Germany). LDL cholesterol was calculated using the Friedewald equation. Prealbumin was measured by common laboratory methods. All samples were analyzed in duplicate.
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