Anti mouse cd45 alexa fluor 647

Manufactured by BioLegend

Anti-mouse CD45-Alexa Fluor 647 is a monoclonal antibody conjugated to the fluorescent dye Alexa Fluor 647. It is designed for the detection and analysis of mouse CD45, a protein tyrosine phosphatase expressed on the surface of most hematopoietic cells.

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Lab products found in correlation

Cd326 ep cam alexafluor 488, by BioLegend (2 mentions) Cd31 alexa fluor 647, by BioLegend (2 mentions) Pdpn apc cy7, by BioLegend (2 mentions) Facsaria cell sorter, by BD (1 mentions) Ly6c apc, by BioLegend (1 mentions) Anti mouse cd31 pe cy7, by BioLegend (1 mentions) Streptavidin apc, by BioLegend (1 mentions) Click it plus edu alexa fluor 647 flow cytometry assay kit, by Thermo Fisher Scientific (1 mentions) Cd140a pdgfrα pe, by BioLegend (1 mentions) 5 ethynyl 2 deoxyuridine edu, by Santa Cruz Biotechnology (1 mentions) Cd45 percp cy5, by BioLegend (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Bromodeoxyuridine (brdu), by BD (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Beyotime (1 mentions) Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions) Beyoclick edu cell proliferation kit with alexa fluor 488, by Beyotime (1 mentions) β actin, by Beyotime (1 mentions) Gapdh, by Proteintech (1 mentions) Pe cy7 rat anti mouse cd45r b220, by BD (1 mentions) Northern lights 3000, by Cytek (1 mentions)

Spelling variants of this product

Anti-CD45 (228 citations) Alexa Fluor 647 (65 citations) Anti-mouse CD45 (39 citations) Alexa 647 (11 citations) Anti-CD45 (Alexa Fluor 647) (2 citations) CD45 (Alexa Fluor 647) (2 citations)

4 protocols using anti mouse cd45 alexa fluor 647

Sorting Cancer Cells with Flow Cytometry

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For sorting of cancer cells, T8 OGO tumors were processed as previously described (Biffi et al., 2019 (link)). Cells were stained for 30 min with anti-mouse CD45-Alexa Fluor 647 (103124; BioLegend), CD326 (EpCAM)-Alexa Fluor 488 (118212; BioLegend), CD31-Alexa Fluor 647 (102416; BioLegend), and PDPN-APC/Cy7 (127418; BioLegend) and for 15 min with DAPI. DAPI/CD45/CD31/PDPN⁻ EpCAM⁺ cells were sorted on the FACSAria cell sorter (BD) and processed for PCR-based genotyping of Trp53 1loxP.

Oni T.E., Biffi G., Baker L.A., Hao Y., Tonelli C., Somerville T.D., Deschênes A., Belleau P., Hwang C.I., Sánchez-Rivera F.J., Cox H., Brosnan E., Doshi A., Lumia R.P., Khaledi K., Park Y., Trotman L.C., Lowe S.W., Krasnitz A., Vakoc C.R, & Tuveson D.A. (2020). SOAT1 promotes mevalonate pathway dependency in pancreatic cancer. The Journal of Experimental Medicine, 217(9), e20192389.

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Sorting and Analyzing Cancer Cells and CAFs

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For sorting of cancer cells and CAFs, KPC tumors were processed as previously described (19 (link)). Cells were stained with anti-mouse CD45-AlexaFluor 647 (103124, BioLegend), CD326 (Ep-CAM)-AlexaFluor 488 (118212, BioLegend), CD31-AlexaFluor 647 (102416, Biolegend), CD140a (PDGFRα)-PE (135905, BioLegend), PDPN-APC/Cy7 (127418, Biolegend) and DAPI for 15 min. Cells were sorted on the FACSAria cell sorter (BD) for DAPI/CD45/CD31- EpCAM+ and DAPI/CD45/CD31/EpCAM- PDPN+ cell populations. For flow cytometry analysis of EdU-treated KPC tumors, KPC mice were administered 300 μg 5-Ethynyl-2-deoxyuridine (EdU) (61135-33-9, Santa Cruz) formulated in sterile saline twice a day for 3 days via intraperitoneal injection. EdU was detected using Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (C10634, Thermo Fisher Scientific). For flow cytometric analysis of IL-1R1 and myCAF/iCAF populations, antibodies employed were: anti-mouse CD31-PE/Cy7 (102418, Biolegend), CD45-PerCP/Cy5.5 (103132, Biolegend), CD326 (Ep-CAM)-AlexaFluor 488, PDPN-APC/Cy7, CD140a (PDGFRα)-PE, Ly6C-APC (128015, Biolegend), biotinylated CD121a (IL-1R, Type I/p80) (113503, Biolegend) and APC Streptavidin (405207, Biolegend).

Biffi G., Oni T.E., Spielman B., Hao Y., Elyada E., Park Y., Preall J, & Tuveson D.A. (2018). IL-1-induced JAK/STAT signaling is antagonized by TGF-β to shape CAF heterogeneity in pancreatic ductal adenocarcinoma.. Cancer discovery, 9(2), 282-301.

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Multiparameter Flow Cytometry Analysis of Cell Populations

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The antibodies used in this study include PE anti-mouse CD24 (BioLegend; 101 808; 1 : 200), PE/Cy7 anti-mouse/human CD44 (BioLegend; 103 030; 1 : 100), FVD (Invitrogen Firable Viability Dye660; 65–0864-14; 1 : 500), Alexa Fluor 647 anti-mouse CD45 (BioLegend; 103 124; 1 : 1000), DAPI (Beyotime; C1006), β-actin (Beyotime; AA128–1; 1 : 5000), goat anti-mouse IgG (H + L) (MULTISCIENCES (LIANKE) BIOTECH, CO., LTD; GAM007; 1:300), anti-rabbit IgG (Cell Signaling Technology; 7074S; 1 : 300), GAPDH (Proteintech; HRP-60004; 1 : 10000), PCNA (Cell Signaling Technology; 2586 T; 1 : 2000), MTT (Beyotime; ST1537-1 g), BeyoClick EdU Cell Proliferation kit with Alexa Fluor 488 (Beyotime; C0071S), bromo-deoxyuridine (BrdU) (BD Pharmingen; 555 627; 1 : 300), Yes1-associated transcriptional regulator (YAP1) (Thermo; RF2213666; 1 : 300), Phalloidin (Beyotime; C2201S 1:300)

Zhang R., Chen S., Yang Z., Zhang N., Guo K., Lv K., Zhou Z., Gao M., Hu X., Su Y., He J, & Wang F. (2023). Actin polymerization inhibition by targeting ARPC2 affects intestinal stem cell homeostasis. Burns & Trauma, 11, tkad038.

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Splenocyte Immunophenotyping by Flow Cytometry

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Freshly prepared splenocytes were incubated in mouse Fc block (Rat anti-mouse CD16/32, BD Biosciences, catalog # 553142, 1:100) for 5 mins followed by incubation in antibodies for pan-leukocytes (Alexa Fluor 647 anti-mouse CD45, BioLegend, catalog # 103124, 1:100), B cells (PE-Cy7 Rat anti-mouse CD45R/B220, BD Biosciences, catalog # 552772, 1:100), and T cells (BV605 anti-mouse CD3, BioLegend, catalog # 100237, 1:100) in dark at 4°C for 30–45 mins, washed with PBS containing 2% FBS. Cells were stored on ice until analyzed using cytometer (CYTEK Northern Lights 3000, Fremont, CA).

Gaire J., Supper V., Montgomery D, & Simmons C.S. (2023). Spiny mice (Acomys) cells fail to engraft in NOD scid gamma. PLOS ONE, 18(5), e0286000.

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