Anti fscn1

Manufactured by Santa Cruz Biotechnology

Anti-FSCN1 is a laboratory reagent that targets the FSCN1 protein. FSCN1 is an actin-bundling protein involved in the organization of the cytoskeleton. Anti-FSCN1 can be used for the detection and study of FSCN1 in various experimental applications.

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Lab products found in correlation

Anti hmb 45, by Santa Cruz Biotechnology (2 mentions) Anti ps6 clone 91b2, by Cell Signaling Technology (2 mentions) Anti sma, by Merck Group (1 mentions) Anti erα, by Agilent Technologies (1 mentions) Anti id 1, by Santa Cruz Biotechnology (1 mentions) Anti pr, by Agilent Technologies (1 mentions) Anti sox9, by Merck Group (1 mentions) Mek162, by Selleck Chemicals (1 mentions) Rictor, by Santa Cruz Biotechnology (1 mentions) Sin 1, by Santa Cruz Biotechnology (1 mentions) Ink128, by Selleck Chemicals (1 mentions) Raptor, by Santa Cruz Biotechnology (1 mentions) E cadherin, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-FSCN1 mouse monoclonal antibody (clone 55 k-2; FSCN1

3 protocols using anti fscn1

Comprehensive Immunohistochemical Profiling

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The antibodies used in this study were anti-ALDH1 (catalog #611194, BD Biosciences), anti-CD61 (#EP2417Y, Novus), anti-ERa (#IR151, Dako), anti-FSCN1 (#SC-56531, Santa Cruz Biotechnology), anti-premelanosome protein (anti-HMB-45; #SC-59305, Santa Cruz Biotechnology), anti-ID1 (#SC-488, Santa Cruz Biotechnology), anti-PR (#IR168, Dako), anti-phospho-Ser235-236 S6 ribosomal protein (anti-pS6; clone 91B2, Cell Signaling Technology), anti-actin alpha-smooth muscle (anti-SMA; #A2547, Sigma-Aldrich), anti-SOX9 (#AB5535, Millipore), and anti-tubulin alpha (TUBA; clone DM1A+DM1B, Abcam). Additional proteins/antibodies were evaluated, but the corresponding immunohistochemistry results were not conclusive of specific signals; they corresponded to anti-epiregulin (anti-EREG; #AF1195, RD Systems), anti-keratin 81 (anti-KRT81; #NBP1-69809, Novus Biologicals), anti-retinoic acid receptor responder 3 (anti-RARRES3; #HPA011219, Sigma-Aldrich), and anti-vascular cell adhesion molecule 1 (anti-VCAM1; #551147, BD Biosciences).

Ruiz de Garibay G., Herranz C., Llorente A., Boni J., Serra-Musach J., Mateo F., Aguilar H., Gómez-Baldó L., Petit A., Vidal A., Climent F., Hernández-Losa J., Cordero Á., González-Suárez E., Sánchez-Mut J.V., Esteller M., Llatjós R., Varela M., López J.I., García N., Extremera A.I., Gumà A., Ortega R., Plà M.J., Fernández A., Pernas S., Falo C., Morilla I., Campos M., Gil M., Román A., Molina-Molina M., Ussetti P., Laporta R., Valenzuela C., Ancochea J., Xaubet A., Casanova Á, & Pujana M.A. (2015). Lymphangioleiomyomatosis Biomarkers Linked to Lung Metastatic Potential and Cell Stemness. PLoS ONE, 10(7), e0132546.

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Immunohistochemical Analysis of Molecular Markers

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The antibodies used in this study were anti-ERα (#IR151, Dako), anti-FSCN1 (#SC-56531, Santa Cruz Biotechnology), anti-HMB-45 (#SC-59305, Santa Cruz Biotechnology), anti ID1 (#SC-488, Santa Cruz Biotechnology), anti-PR (#IR168, Dako), anti-phospho-Ser235-236 S6 ribosomal protein (anti-pS6; clone 91B2, Cell Signaling Technology), anti-SMA (#A2547, Sigma-Aldrich), and anti-SOX9 (#AB5535, Millipore).

Nuñez O., Román A., Johnson S.R., Inoue Y., Hirose M., Casanova Á., de Garibay G.R., Herranz C., Bueno-Moreno G., Boni J., Mateo F., Petit A., Climent F., Soler T., Vidal A., Sánchez-Mut J.V., Esteller M., López J.I., García N., Gumà A., Ortega R., Plà M.J., Campos M., Ansótegui E., Molina-Molina M., Valenzuela C., Ussetti P., Laporta R., Ancochea J., Xaubet A., Pollán M, & Pujana M.A. (2016). Study of breast cancer incidence in patients of lymphangioleiomyomatosis. Breast Cancer Research and Treatment, 156, 195-201.

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Molecular Pathway Protein Analysis

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INK-128 and MEK-162 were obtained from Selleck (Shanghai, China). Five-fluorouracil (5-FU) was purchased from Sigma (St Louis, MO).Anti-FSCN1, Erk1, Akt1, mTOR, Raptor, Sin1, E-Cadherin, β-actin, Rictor and tubulin antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). All other kinase antibodies used in this study were obtained from Cell Signaling Technology (Shanghai, China).

Li C., Cui J.F., Chen M.B., Liu C.Y., Liu F., Zhang Q.D., Zou J, & Lu P.H. (2015). The preclinical evaluation of the dual mTORC1/2 inhibitor INK-128 as a potential anti-colorectal cancer agent. Cancer Biology & Therapy, 16(1), 34-42.

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