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9 protocols using carbopol 934

1

Aceclofenac Formulation Development

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Aceclofenac (99.97% pure with loss on drying 0.34%) was obtained as a gift from Time Pharmaceuticals Pvt. Ltd, Nepal. Carbopol 934 (CP) was purchased from Himedia Laboratories India. Hydroxypropyl methylcellulose (HPMC) and sodium carboxymethylcellulose (SCMC) were purchased from Loba Chemie Pvt. Ltd, Mumbai. Magnesium stearate, micro crystalline cellulose powder 200 (MCCP 200), and talc were purchased from Sigma-Aldrich, Inc. (St Louis, MO, USA). All the chemicals and reagents used were of analytical grade.
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2

Extracellular Matrix Protein Analysis

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Glycine, phenazine methosulfate fluoride (PMSF), Bradford reagent, and Tris-HCl were purchased from Sigma (Sigma Chemical Co., USA). Carbopol 934, propylene glycol, gentamicin sulfate, l-hydroxyproline, and p-dimethyl-amino-benzaldehyde were obtained from Himedia (Himedia Pvt. Labs., India). bFGF, COL3A1, Smad-2, Smad-3, Smad-4, and Smad-7, and β-actin primary and secondary antibodies and BCIP- (5-bromo-4-chloro-3′-indolyphosphate p-toluidine salt-) NBT reagents were procured from Santa Cruz (Santa Cruz, USA). PVDF membrane was from Millipore (Millipore Corp., USA) and Western Max-HRP-Chromogenic detection kit was purchased from Amresco (Amresco, USA). All other chemicals and reagents not mentioned were of the analytical grade.
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3

Levofloxacin-Based Eye Drop Formulation

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Levofloxacin, BSP, poloxamer 188 (Sigma Chemical Co. [Aldrich], USA). Xanthan gum, Carbopol 934, Methyl cellulose (HIMEDIA, India). Levofloxacin eye drop (purchased from local pharmacy trade name QUIXIN, company Vistakon). Betamethasone eye drops (purchased from local pharmacy trade name Betnesol™, Company Gsk). All other solutions, chemicals and reagents were of analytical grade.
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4

Formulation and Evaluation of Frovatriptan Succinate Topical Gel

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Frovatriptan succinate (CAS Number: 158930-17-7) was purchased from Pharmaffiliates (Catalogue number: PA 06 83000, India), Poloxamer 407 was purchased from Sigma-Aldrich® Solutions, Germany (CAS Number: 9003-11-6, ID: 16758), Carbopol 934 was purchased from HiMedia Laboratories LLC, USA (stock keeping unit: GRM6761), (Hydroxypropyl)methyl cellulose K100 was purchased from Otto Chemie Pvt. Ltd., India (CAS: 9004-65-3, HSN Code: 39123919, and Code: H 1815), and Dialysis Membrane-135 was purchased from HiMedia Laboratories LLC, USA (stock keeping unit: LA398), phospholipon ® 90H [Phospholipids, hydrogenated, with 70% phosphatidylcholine] was purchased from Pharma Excipients ™ (CAS-No. 97281-48-6), the remaining chemicals and reagents were of analytical grade and procured from local vendors.
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5

Transdermal Finasteride and Testosterone Formulation

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Chitosan (low molecular weight), sodium alginate, calcium chloride, acetic acid, sodium hydroxide, carbopol 934, methyl paraben, propyl paraben, propylene glycol, triethanolamine, finasteride (all were procured from Hi-Media laboratories, Mumbai, India), testosterone (Loba Chemie Pvt Ltd., Mumbai, India), and carboxymethylcellulose (Sigma-Aldrich) were used. All chemicals and solvents used were of analytical reagent grade.
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6

Emulgel Formulation and Characterization

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Gel base was prepared by dispersing carbopol 934 (Hi-media, India) in distilled water with constant stirring at moderate speed for overnight. Oil phase of the emulsion was made with cetomacrogol 1000, cetostearyl alcohol, white soft paraffin, propylene glycol in light liquid paraffin (Hi-media, India). Aqueous phase was prepared by dissolving peptides (0.5% w/v) in distilled water. The oily and aqueous phases were separately incubated in a water bath at 60°C. Upon obtaining uniformity, oily phase was added to the aqueous phase with continuous stirring. Pre-heated gel base dispersion of carbopol 934 and vitamin ETPGS (Sigma, United States) were added to the emulsion steadily with continuous stirring to obtain homogenized gel. The temperature of the formulation was lowered to 40°C and isopropyl alcohol was added with continuous stirring. The pH was maintained at 6.8 ± 0.2 using saturated solution of citric acid. In vitro emulgel was characterized for its visual appearance under the microscope for the presence of any particle or grittiness. The pH of emulgel was determined by digital pH meter (Contech, India) by dissolving gel in distilled water. Gel extrudability or oozing out from the collapsible tube was determined by physical squeezing from a tube. Gel viscosity was measured using viscometer (Brookfield DV-l Prime, United States).
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7

Transdermal Oleic Acid-Curcumin Formulation

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Oleic acid (cis-9-octadecenoic acid), curcumin, and silica gel 60 were purchased from Sigma-Aldrich (Steinheim, Germany). Carbopol-934 was purchased from HiMedia Laboratories (Mumbai, India). Sulfadiazine (Flamazine) was obtained from Riyadh Pharmaceutics, Riyadh, Kingdom of Saudi Arabia.
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8

Formulation and Evaluation of Topical Tretinoin-BPO Gel

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Tretinoin was procured ex gratis from Shalaks Pharmaceutical, New Delhi, India, while BPO was gifted by the H.K. Group, Mumbai, India. Span 60 and oleic acid were obtained from SD Fine Chemicals Limited, Mumbai, India, and cholesterol was obtained from Central Drug House (P) Ltd, New Delhi, India. Isopropanol was obtained from the Central Drug House (P) Ltd, carbopol 934 from Hi-media Laboratories PVT Ltd, Mumbai, India, and phosphotungstic acid from the Central Drug House (P) Ltd. All other materials and chemicals were of analytical grade.
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9

Formulation and Characterization of RP

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RP was a generous gift from Wockhardt Pharmaceuticals Pvt. Ltd., Aurangabad, India. Propylene glycol monocaprylate (Capryol ® 90), tripalmitin (Dyanasn™ 114), Poloxamer 188, polyoxyethylene sorbitan monolaurate (Tween ® 20), and Centrisart filters (10 kDa molecular weight) were purchased from Sigma Aldrich Chemicals Pvt Ltd., Bangalore, India. Carbopol ® 934 was procured from Hi-Media, Mumbai, India. Other glassware and chemicals required for this project like High-Performance Liquid Chromatography (HPLC) grade solvents were obtained from Merck Hyderabad, India.
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