Live death vivid detection kit
The Live/Death Vivid detection kit is a laboratory tool used to distinguish between live and dead cells. It provides a straightforward method for quickly assessing cell viability through fluorescent staining. The kit includes fluorescent dyes that selectively stain live and dead cells, enabling the user to visualize and quantify the population of each cell type.
Lab products found in correlation
3 protocols using live death vivid detection kit
Multiparametric Flow Cytometry Analysis of PBMCs
Comprehensive B-cell Immunophenotyping by Flow Cytometry
Multicolor Flow Cytometry of T-cell Subsets
Intracellular staining was carried out using the FoxP3 permeabilization solution kit according to the manufacturer's instructions (eBioscience, Paris, France) together with PE Ki67, PE anti-Bcl-6 (BD Pharmingen) or PE anti-FoxP3 (Biolegend), and Alexa-Fluor 488 anti-SAMHD1 (a gift from O. Schwartz) or rabbit anti-SAMHD1 (Euromedex, Souffelweyersheim, France) followed by Alexa-Fluor 647 antirabbit antibody (Invitrogen, Life Technologies, Saint Auben, France).
Dead cells were excluded using the Live/Death Vivid detection kit labeled with an aqua dye (Invitrogen). Fluorescence intensities were measured with an LSR II flow cytometer (Becton Dickinson) and analyzed using FlowJo version 7.6.5 (Tree Star Inc., Ashland, Oregon, USA).
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