Log In Sign up
The largest database of trusted experimental protocols

Cmv renilla luciferase plasmid

Manufactured by Promega
Visit Supplier
Sourced in United States

The CMV-Renilla luciferase plasmid is a laboratory tool used to express the Renilla luciferase enzyme under the control of the cytomegalovirus (CMV) promoter. The Renilla luciferase enzyme can be used as a reporter to monitor gene expression or cellular processes in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Dual luciferase kit, by Promega (1 mentions) Ecl system, by PerkinElmer (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Gotaq dna polymerase, by Promega (1 mentions) Np 40, by Merck Group (1 mentions) Retroscript kit, by Thermo Fisher Scientific (1 mentions) 100 bp dna ladder, by Thermo Fisher Scientific (1 mentions) T4 polynucleotide kinase, by Promega (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Igf 1, by Merck Group (1 mentions) Dnase 1, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Proteinase k, by Merck Group (1 mentions) Formaldehyde, by Merck Group (1 mentions) Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions)

Close spelling variants of this product

PRL-CMV Renilla luciferase plasmid PRL-CMV plasmid-expressing renilla luciferase PRL-CMV renilla luciferase reporter plasmid Renilla luciferase plasmid Renilla luciferase Renilla plasmid

3 protocols using cmv renilla luciferase plasmid

1

Cell Culture and Molecular Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DMEM, 100 bp DNA ladder, l-glutamine, penicillin, streptomycin, bovine serum albumin and phosphate-buffered saline were purchased from Invitrogen (Carlsbad, CA, USA), and Sephadex G50 spin columns and poly (dI-dC) from Roche (Indianapolis, IN, USA). GoTaq DNA polymerase, T4 polynucleotide kinase, dual luciferase kit, FuGENE 6 and CMV renilla luciferase plasmid were provided by Promega (Madison, WI, USA). The RETROscript kit and DNase I were purchased from Ambion (Austin, TX, USA). Aprotinin, leupeptin, phenylmethylsulfonyl fluoride, sodium orthovanadate, formaldehyde, NP-40, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), dimethyl sulfoxide, proteinase K, tRNA, IGF-1, DHT, bicatulamide(Cx) were from Sigma (Milan, Italy). Antibodies against cyclin D1, p21, GAPDH, and polymerase II (N20) were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody anti-ER beta (clone 64–4, rabbit monoclonal immunoglobulin G (IgG), 1:1000 dilution) was purchased from Millipore. The ECL System, 3H thymidine and [γ32P]ATP were purchased from PerkinElmer (Wellesley, MA, USA), salmon sperm DNA/protein A agarose was from UBI (Chicago, IL, USA), and triazol, SYBR Green Universal PCR Master Mix was from Biosystems (Forster City, CA, USA).
Rizza P., Barone I., Zito D., Giordano F., Lanzino M., De Amicis F., Mauro L., Sisci D., Catalano S., Wright K.D., Gustafsson J.A, & Andò S. (2014). Estrogen receptor beta as a novel target of androgen receptor action in breast cancer cell lines. Breast Cancer Research : BCR, 16(1), R21.
+ Open protocol
+ Expand
2

Transfecting Cells via Electroporation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Invitrogen Neon Transfection System (Life Technologies; Carlsbad, CA) was used according to manufacturer’s instructions to transfect cells via electroporation. Each transfection contained 106 cells and 120ng of plasmid DNA comprised of 10ng CMV-GFP plasmid (Promega; Madison, WI), 10ng CMV-Renilla luciferase plasmid (Promega; Madison, WI), 100ng ERE-firefly luciferase plasmid. The ERE-Firefly luciferase (3× ERE TATA luc) plasmid was a gift from Donald McDonnell (Addgene plasmid # 11354) (Hall & McDonnell 1999 (link)). Two pulses, each 1400V and 20 milliseconds, were applied to the transfection mixture and cells were plated in a 96-well plate at a seeding density of 1.05×105 cells per cm2 in antibiotic-free growth media. Cells were allowed to recover in growth media for 40 hours after electroporation and prior to steroid hormone starvation. Cultures were then hormone starved for 24 hours prior to treatment as described in Section 2.1.2.
Pollard K.J, & Daniel J.M. (2019). Nuclear estrogen receptor activation by insulin-like growth factor-1 in Neuro-2A neuroblastoma cells requires endogenous estrogen synthesis and is mediated by mutually repressive MAPK and PI3K cascades. Molecular and cellular endocrinology, 490, 68-79.
+ Open protocol
+ Expand
3

WNT Signaling Pathway Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were co-transfected with SUPER8XTOPFlash TCF/LEF-firefly luciferase reporter plasmid (Kind gift from Dr Muy-Teck Teh), CMV-Renilla luciferase plasmid (Promega) and any other plasmids indicated in figures. 24 hours later, cells were stimulated with 100 ng/mL recombinant WNT3A (R&D), 100 ng/mL recombinant WNT5A (R&D), 5 μM BIO (Sigma) or their respective vehicles for 24 hours before luciferase activity was measured using the Dual Luciferase Reporter assay system (Promega).
Thorup A.S., Strachan D., Caxaria S., Poulet B., Thomas B.L., Eldridge S.E., Nalesso G., Whiteford J.R., Pitzalis C., Aigner T., Corder R., Bertrand J, & Dell'Accio F. (2020). ROR2 blockade as a therapy for osteoarthritis. Science translational medicine, 12(561).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!