Human vcam 1 picokine elisa kit

Venous blood samples were collected from patients and control subjects after 10-12 hours of overnight fasting under complete aseptic conditions. Complete blood count, random blood glucose, and ESR were determined. The serum was separated within one hour of collection. Lipid profile and rheumatoid factor (RF), CRP, anti-cyclic citrullinated peptide (anti-CCP) antibodies, urea, and creatinine were measured for patients and control subjects. The rest of the serum was stored at -80˚C until further analysis. The level of serum VCAM-1 was measured by Human VCAM-1 PicoKine™ ELISA Kit (Boster Biological Technology, USA, Catalog number EK0537) according to the manufacturer’s instructions. Total antioxidant capacity and MDA were measured in the serum using colorimetric kits (Biodiagnostic Company, Dokki, Giza, Egypt). The atherogenic index was calculated according to the following formula: Log [triglycrides (TG)/high-density lipoprotein (HDL)].^{17 (link)}